1.STUDY ON SCAVENGING FREE RADICALS BY ACTIVE COMPOUNDS FROM BARLEY LEAVES
Acta Nutrimenta Sinica 1956;0(04):-
Objective: To study the scavenging capacity of extract from four kinds of barley leaves on superoxide free radical and hydroxyl free radical. Methods: Measure the content of superoxide free radical and hydroxyl free radical by nitro blue tetrazolium(NBT) photo-reduction. Results: Along with the flavonoid in the reaction liquid, the scavenging rate shows ascending trend . When the concentration of flavonoid is 12?g/ml, its scavenging rate is 95.56% and 94.12% on superoxide free radical and hydroxyl free radical respectively. Conclusion: The flavonoids of barley leave s have stronger anti-oxidative action.
4.Midline carcinoma with rearrangement of nuclear protein in testis gene.
Chinese Journal of Pathology 2011;40(3):209-212
Carcinoma
;
drug therapy
;
genetics
;
metabolism
;
pathology
;
radiotherapy
;
Desmoplastic Small Round Cell Tumor
;
metabolism
;
pathology
;
Diagnosis, Differential
;
Gene Rearrangement
;
Head and Neck Neoplasms
;
drug therapy
;
genetics
;
metabolism
;
radiotherapy
;
Humans
;
Keratin-20
;
metabolism
;
Keratin-7
;
metabolism
;
Lymphatic Metastasis
;
Male
;
Mediastinal Neoplasms
;
drug therapy
;
genetics
;
metabolism
;
radiotherapy
;
Melanoma
;
metabolism
;
pathology
;
Neuroectodermal Tumors, Primitive
;
metabolism
;
pathology
;
Nuclear Proteins
;
genetics
;
metabolism
;
Oncogene Proteins
;
genetics
;
metabolism
;
Oncogene Proteins, Fusion
;
genetics
;
metabolism
;
Rhabdomyosarcoma
;
metabolism
;
pathology
;
Thymus Neoplasms
;
drug therapy
;
genetics
;
metabolism
;
radiotherapy
5.Extranodal IgG4-positive nasal/paranasal sinus node Rosai-Dorfman disease : report of a case.
Chinese Journal of Pathology 2013;42(2):126-127
Aged
;
Antigens, CD
;
metabolism
;
Antigens, Differentiation, Myelomonocytic
;
metabolism
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Histiocytosis, Sinus
;
pathology
;
surgery
;
Humans
;
Immunoglobulin G
;
blood
;
Paranasal Sinus Diseases
;
pathology
;
surgery
;
Paranasal Sinuses
;
pathology
;
S100 Proteins
;
metabolism
;
Sclerosis
;
pathology
7.Progress in research on DNA methylation and laryngeal carcinoma.
Chinese Journal of Pathology 2011;40(1):67-70
Acid Anhydride Hydrolases
;
genetics
;
metabolism
;
Animals
;
Antimetabolites, Antineoplastic
;
pharmacology
;
Azacitidine
;
analogs & derivatives
;
pharmacology
;
Biomarkers, Tumor
;
analysis
;
Carcinoma, Squamous Cell
;
diagnosis
;
genetics
;
metabolism
;
pathology
;
Cyclin-Dependent Kinase Inhibitor p16
;
genetics
;
metabolism
;
DNA Methylation
;
drug effects
;
Genes, p16
;
Humans
;
Laryngeal Neoplasms
;
diagnosis
;
genetics
;
metabolism
;
pathology
;
Neoplasm Proteins
;
genetics
;
metabolism
;
Tumor Suppressor Proteins
;
genetics
;
metabolism
8.The role of receptor protein tyrosine kinase MERTK and intracellular Ca2+ playing in the phagocytosis of human retinal pigment epithelial cells
Yuzhao SUN ; Jing HONG ; Gang AN
Chinese Journal of Ocular Fundus Diseases 2009;25(3):193-197
Objective To investigate the role of intraeellular Ca2+ and MERTK in the phagocytosis of human retinal pigment epithelial (RPE) cells, and reveal the relationship between MERTK and intraeellular Ca2+. Methods The euhured RPE cells were incubated with rod outer segments (ROS) at 37℃, the phagoeytosis was terminated at different incubation time points. The concentration of intraeellular Ca2+ was assayed by Fluro-3/AM loading methods combined with fluorescence microscope and CCD system, and the mRNA level of MERTK gene was measured by reverse transcription polymerase chain reaction (RT-PCR). Treating the RPE cells with stimulator (A23187)or inhibitor(verapamile)of intraeellular Ca2+ to observe the changes of MERTK gene expression. Results ROS adhered to hRPE cells at the 15th minute, and the ingestion saturated at the 24th hour. The concentration of intracellular Ca2+ increased at the 15th minute, and kept the high level in 24 hours. The level of MERTK mRNA increased at the 5th minute, and kept the high level duration the whole incubation. When RPE cells were treated by A23187, the expression of MERTK increased in a dose-dependent manner. After RPE cells was pretreated by A23187, the expression level of MERTK was higher in the proceeding incubation groups than which in the control group except at the 3rd hour. When RPE cells were treated by verapamil, the expression level of MERTK decreased in a dose-dependent manner. After RPE cells were pretreated by verapamil , the expression level of MERTK was lower in all the proceeding incubation groups than which in the control group (P<0.05). Conclusion MERTK gene and Ca2+ play an important role in sustaining RPE cells phagoeytizing ROS. As an up-stream regulator, the receptor tyrosine kinase MERTK keeps RPE cells phagocytizing ROS by starting the intracellular Ca2+.
9.Recent advances in adenoid cystic carcinoma of minor salivary gland.
Chinese Journal of Pathology 2011;40(11):783-787
Adenocarcinoma
;
pathology
;
Adenoma
;
pathology
;
Carcinoma, Adenoid Cystic
;
epidemiology
;
genetics
;
metabolism
;
pathology
;
Carcinoma, Basosquamous
;
pathology
;
DNA, Mitochondrial
;
genetics
;
Diagnosis, Differential
;
Humans
;
Immunohistochemistry
;
Incidence
;
Proto-Oncogene Proteins c-kit
;
metabolism
;
Salivary Gland Neoplasms
;
epidemiology
;
genetics
;
metabolism
;
pathology
;
Salivary Glands, Minor
;
pathology
;
beta-Defensins
;
genetics
10.A Review for Progress of SELDI Protein Chip Technique Applications
Ke MA ; Hong-Gang ZHANG ;
China Biotechnology 2006;0(08):-
From genomics to proteomics, and then to miRNA, all without exception showed that proteins are the substance which directly regulate life. Comparing with genomics, the study of proteins is more difficult for its great variety, complicated modification and complicated construction. Recently, some new techniques for protein assay and research have provided us, for example, Fluorescence labeling, SELDI, SPR and optical protein chip, of which, SELDI SPR and optical protein chip are label-free. SELDI protein chip technique and its newly developed applications are briefly reviewed here.