Objective: To study the effect of siRNA on multidrug resistance1(MDR1) gene expression in KBv200 cells. Methods: MDR1-siRNA was synthesized and transfected into KBv200 cells.The MDR1 mRNA and P-gp were assayed by RT-PCR and flow cytometry.Cytotoxicity was determined by a tetrazolium-based chemosensitivity assay(MTT).Cellular accumulation of Dox was measured by fluorescence spectrophotometry. Results: MDR1-siRNA decreased MDR1 mRNA and P-gp expression(P

According to the base complementation, the principle antisense technology is the sequence specific binding of complementary antisense nucleic acids or their chemical modification to the nucleic acids in cells, resulting in the suppression or prevention of gene translation. For the treatment of cancer, a lot of antisense drugs have been designed, which target the genes involved in the pathogenesis of cancer. And some of them have been in clinical trials. The author reviewed the progress of the clinical trials of these antisense drugs.

Carcinoma ; drug therapy ; genetics ; metabolism ; pathology ; radiotherapy ; Desmoplastic Small Round Cell Tumor ; metabolism ; pathology ; Diagnosis, Differential ; Gene Rearrangement ; Head and Neck Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy ; Humans ; Keratin-20 ; metabolism ; Keratin-7 ; metabolism ; Lymphatic Metastasis ; Male ; Mediastinal Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy ; Melanoma ; metabolism ; pathology ; Neuroectodermal Tumors, Primitive ; metabolism ; pathology ; Nuclear Proteins ; genetics ; metabolism ; Oncogene Proteins ; genetics ; metabolism ; Oncogene Proteins, Fusion ; genetics ; metabolism ; Rhabdomyosarcoma ; metabolism ; pathology ; Thymus Neoplasms ; drug therapy ; genetics ; metabolism ; radiotherapy

Objective To discuss the impact of zedoary turmerie oil(ZTO) injection over oncosis index and Bcl-2 expression of human ovarian cancer SKOV3 cell.Methods After ZTO injection acted on human ovarian cancer SKOV3 cells,the changes in the nucleus were observed by fluorescence microscope,oncosis index was counted by projection electron microscope,the situation of cell DNA breakage was observed by agarose gel electrophoresis,and cell Bcl-2 gene expression was observed by immunohistochemical method.Results After treated by ZTO injection for 48 hours,human ovarian cancer SKOV3 cells shows that oncosis cell was swelling in fluorescence microscope,the volume increased,the membrane area narrowed,the nuclear chromatin expansed.The oncosis index increased with dose-effect relationship,DNA electrophoresis showed diffuse type and Bcl-2 gene expression was down-regulated.Conclusion ZTO injection can increase oncosis index of human ovarian cancer SKOV3 cell with the concentration,and lower Bcl-2 gene expression,which may be one of the mechanisms of ZTO injection caused oncosis of SKOV3 cell.

Objective: To determine the component of naphtha from Acorus tatarinowii Schott.which can pass through the blood-brain barrier. Methods: Naphtha in rat brain was analyzed by GC-MS after gastric infusion of naphtha. Results: The methylisoeugenol,elemicin, ?-asarone and ?-asarone were detected in rat brain. Conclusion: The resuscitative effect of naphtha is resulted from the comprehensive action of multiple components.

[Objective] To analyze the main chemical constituents of decoction and concentrated decoction of Rhizoma Acori Tatarinowii (RAT) by gas chromatography-mass spectrometry (GC-MS). [Methods] RAT was decocted and concentrated in the pottery for two times and then 6 batches of the decoction and its concentrated decoction were analyzed by GC-MS. [Results] Five kinds of components in a higher amount were found in the first and second decoction of RAT, including: ?-asarone, ?-asarone, 2, 3-dihydro-3, 5-dihydroxy-6-methyl-4H-pyran-4-one, 5-hydroxymethylfurfural and acoramone. The contents of volatile components of ?-asarone and ?-asarone were lower while those of water-soluble components higher in the concentrated decoction of RAT. [Conclusion] The therapeutic effect of RAT is the co-action of the multiple components of RAT; the effect is related not only with the volatile components but also with the water-soluble components. Therefore, more attention should be paid to the difference of components in the concentrated decoction, which is generally used in the research of new Chinese herbal medicine, and in the clinically used decoction.

Objective: To screen the extraction process of volatile oil in Supplemented Xanthium Powder.Methods: The main compositions of volatile oil contained by every herb in prescription were followed up by GC MS. Results: The extraction process of volatile oil in Supplemented Xanthium Powder was established, that is, Flos Magnoliae and Cortex Moutan Radicis were powdered, their volatile oils were extracted with Herba Menthae together by the steam distillation; then 13 fold first fraction of distillata and 3 fold redistillation liquid were collected respectively; the volatile oil of Rhizoma Liqustici Chuanxiong was extracted with alcohol. Inasmuch as Fructus Xanthii and Radix Angelicae Dahuricae contained less volatile oil, at the same time, to avoide that Fructus Xanthii produced toxicity by water extraction and coumarinic lactones of Radix Angelicae Dahuricae was destroyed their oils were considered unfit for extraction. Conclusion: GC MS is a useful way for follow up optimization of the extraction process of volatile oil in compound prescription of Chinese medicinal and worthy of popularization.

Objective To explore the effect of Zn 2+ on human leukemia promyelocyte (HL 60)cycle changes induced by multiglucoside tripterygii (MT).Methods ① HL 60 phenotype and membrane protein Fas expression was detected.② HL 60 was cultured with MT observing the cell forms and cell cycle changes,DNA electrophoresis results and the effect of Zn 2+ on the cell cycle change.Results ① The HL 60 were CD4 +CD8 -CD19 -IFN ? +IL 8 -,and the Fas protein expression was poorer.② Following culture of HL 60 with MT,the apoptosis relative cell cycle change appeared in HL 60.This change mainly affected G 2/M and S stage cells,and presented dose time correlation.③ Zn + nearly all blooked the effect of MT induced HL 60 cell cycle change.Conclusion ① HL 60 has Th1 lymphocyte like characteristics,and the Fas protein expression is poorer.② Zn + affects the MT induced HL 60 cell cycle change.It suggests that Zn + might affect the therapeutic and side effects during clinical treatment of many autoimmune diseases with MT.

Objective: To determine ? asarone and ? asarone in Rhizoma acori tatarinowii(RAT) . Methods: HPLC condition consists of ODS C 18 column(150mm?4.6mm, 5?m), methanol: water(6∶4) as mobile phase containing potassium dihydrogen phosphate 1.4g and sodium lauryl sulfate 1.2g per 1000mL, detective wavelength at 257nm, flow rate at 1.0mL?min -1 . Results: For RAT the mean recovery of 99.02%( RSD =1.03%) for ? asarone, 101.26%( RSD =3.57%) for ? asarone are obtained, respectively. Conclusion: The method is sensitive, rapid and accurate.

Objective To study the influence of Mutouhui extracts on hemorheology of U14 cervical cancer mice.Methods The U14 cervical cancer mice were divided into Mutouhui extracts high,middle and low dose group,Cisplatin Injection group,model group and blank comparing group.The influence on hemorheology change was observed after being given the medicine.Results The high,middle dose group of Mutouhui extracts and Cisplatin injection group could reduce the blood viscosity of U14 cervical cancer mouse in different shear,and hemotcrit of the low did not obviously change,groups of Mutouhui extracts plasma viscosity changed slightly.Cisplatin Injection group could reduce the blood viscosity,but did not obviously affect to the plasma viscosity.Conclusion Mutouhui extracts can reduce the U14 cervical cancer mouse blood viscosity,and improve the indexes of hemorheology.