Objective To investigate the clinical efficacy and safety of Ganluxiaodu micropills in treatment of helicobacter pylori(Hp)infected chronic atrophic gastritis.Methods 120 patients with chronic atrophic gastritis (pixu shire syndrome)from April 2013 to April 2014 were selected and divided into observation group and the control group (n =60)according to random number table method.The control group was given standard triple therapy,the observation group was given Ganluxiaodu micropills on the basis of the control group,7 days for a period of treatment. The symptoms changes of the two groups before and after treatment were observed and combined results of the gastro-scope and breath test,determined the clinical curative effect and eradication rate of Hp.The SF -36 scale score was used to evaluate the quality of life of the patients.Results The clinical symptom scores[(15.92 ±2.25)points vs (16.12 ±2.14)points ],SF -36 scale [(68.05 ±12.98)points vs (65.77 ±11.34)points]of the two groups before treatment had no statistically significant differences (t =-1.033,-1.239,all P >0.05).The clinical symp-tom scores and SF -36 score of the observation group and the control group after treatment [(8.24 ±1.23)points, (89.44 ±12.93)points;(12.65 ±1.75)points,(77.43 ±11.59)points ]were obviously improved than before treat-ment,the differences were statistically significant (t =2.574,2.765,2.133,2.047,all P <0.05);the observation group improved significantly better than the control group,the difference was statistically significant (t =2.394, 2.432,all P <0.05 ).The total effective rates of observation group and control group were 96.7% and 83.3%respectively,Hp eradicate rates were 93.3%,70.0% respectively,which in the observation group were significantly higher than the control group,the differences were statistically significant(χ2 =4.282,4.098,all P <0.05).There were no serious adverse reaction happened in the two groups,the incidence rate of adverse reactions of the two groups (11.67% vs 8.33%)had no statistically significant difference (χ2 =1.032,P >0.05).Conclusion Ganluxiaodu micropills can improve the Hp eradicate rate of standard triple therapy,the clinical efficacy of Ganluxiaodu micropills in treatment of Hp infected chronic atrophic gastritis (pixu shire syndrome)is significant,which is worthy of clinical popularization and application.

Objective To evaluate the prognosis of tongue carcinoma surgery with preservation of the integrity of mandible and dentition .Methods Sixty four cases of tongue carcinoma surgery from January 2009 to January 2011 were randomly collected (sev‐en missed ,57 enrolled) .Through retrospective case‐control study on retention of mandibular dentition and mandible rectangle radi‐cal operation of tongue carcinoma radical operation of tongue cancer resection were analyzed the clinical data including the survival rate ,reoccurrence rate and post‐operation life quality .The questionnaire was conducted by fourth edition of UW‐QOL .Results There were no significant difference in survival rates of the two groups of patients between retaining the mandible and dentition and resection of mandible rectangle (P>0 .05) .Two different types of surgical procedures for treatment of jaw had no correlation with the reoccurrence rate (P>0 .05) .The quality of life in retaining the mandible and dentition was significantly higher than the rectan‐gular groups (P<0 .05) .Conclusion Under a strict choice of surgical indications ,preserving radical operation of tongue carcinoma of the mandible and dentition could achieve good results of surgical treatment ,and can significantly improve patients′quality of life after operation .

Irreversible electroporation, known as nano-knife, is a newly-developed technology, which can effectively ablate tumor tissues. This technology uses electric pulse to permanently destroy the double phospholipid layer of the cell membrane in the target area, resulting in tumor cell death. Its tumor ablation effect has already been confirmed in animal experiment as well as in clinical study. As this technology has no thermal effect, the adjacent connective tissue matrix such as blood vessels, nerves and biliary ducts will not be damaged. This article aims to make a brief review about its technical principle and apparatus, and its advantages, disadvantages as well as the recent progress in clinical research will also be discussed.

In the initial experiment for the evaluation of the correlativity between McAb-and PcAb-ELISA systemes, we found that the serum IgE levels detected by the McAb-ELISA were about 10 times lower than those detected by the PcAb-ELISA system. Subsequent works demonstrated that this is due to the cross-reaction of PcAb with some non-IgE component(s) in human serum whereas the the possibility that the epitopes directed by our McAbs are not universally present on all IgE molecules, although ean not be definitely excluded at the present time, may not be responsible to the differences.

The effects of methionine-enkephalin (M-EnK) on mitogenic and mixed lymphocyte culture (MLC) proliferation of splenocytes from Zn-deficient, restricted and control mice were evaluated. The data from this experiment showed that M-Enk could suppress the responses of splenocyte from th 3 groups to eoncanavalin A(Con A), but less inhibition was observed in the Zn-deficient group. M-Enk could also enhance the responses to Pokeweed Mitogen (PWM) and decrease the response to Lipopolysaceharide (LPS) in all groups. Alteration of proliferative responses to LPS,Con A and FWM was reversible in the presence of 10 ?mol/L naloxone, indicating that the effect of M-Enk on cellular proliferation was mediated by the opioid receptor. In the proliferation of MLC, the response of lymphocytes from Zndeficient mice was increased in the absence of M-Enk and M-Enk could suppress this increased response. It is therefore concluded that Met-Enk can modify the pattern of mitogenic responses and the alteration in Con A and MLC responses can be influenced by zinc deficiency.

The dynamic changes of immunoreactivr argininc vasoprrssin (Jr-AVP) contents in the plasma and some tissues in burned rats were observed in this study.Radioimmuno-assay showed that the ir-AVP contents in tho plasma were significantly increased immediately,, 60, 120, 240 min after burn while in the posterior pituitary the ir-AVP content was significantly decreased, suggesting the release of AVP from the posterior pituitary into the plasma in burn shock.The ir-AVP contents in.the hypothalamus, anterior pituitary, pons-medulla oblongata, spinal cord and adrenal gland also displayed changes to different extent under burn shock condition, the significance of which needs further study.

The effects of ginsenosides(including the total ginsenoside extracted from stems and leaves-GNS, ginsenoside extracted from rots-GRS, panaxadiol saPonin-PDS and panaxatriol saponin-PTS) on the activities of Na+, K+-ATPase of dog heart sarcolemma were studied in vivo and in vitro.Both GNS and GRS showed dramatic inhibitory effects on the activities of Na+, K+-ATPase at 10, 20 and 40 mg/kg .The activities of Na+, K+-ATPase were also significantly inhibited by PDS and PTS at 5, 10 and 20 mg/kg. In vitro, all the ginsenosides used in this experiment, at the concentrations of 0.01, 0.10 and 1.00 g/L, Possessed significant inhibitory effects on the activities of dog heart sarcolemmal Na+, K+-ATPase .These results indicated that the enhancement of the contractility of cardiac muscle by ginsenosides is associated with its inhibition on the activities of Na+, K+-ATPase .

OBJECTIVE:To study the protective effect of Mogrosides on experimental liver injury in mice. METHODS: Acute hepatic injury model was induced by CCl4 in mice, and the immunological hepatic injury model was induced in mice by bacillus calmette-guerin (BCG) and lipopolysaccharide (LPS). The activities of ALT and AST in serum, SOD activity in liver tissue and content of MDA in the liver tissue were investigated; and the histological changes of liver were observed. RESULTS: Mogroside could decrease the serum activities of ALT and AST in mice with acute hepatic injury or immunological hepatic injury, increase the activity of SOD but decrease MDA content in liver tissue of mice with immunological hepatic injury, and it could lessen the histological changes of liver. CONCLUSION: Mogroside showed protective effect on mice with acute hepatic injury or immunological hepatic injury. The mechanism might be attributed to its action against lipid peroxidation.

OBJECTIVE:To study the pharmacokinetic characteristics of esomeprazole capsules versus esomeprazole tablets in healthy volunteers and to evaluate their bioequivalence.METHODS:A single oral dose of 40 mg esomeprazole capsules or esomeprazole tablets was given to 24 healthy male volunteers who were randomly assigned to two groups in an open randomized crossover test.The plasma concentration was determined by HPLC.The pharmacokinetic parameters of esomeprazole were analyzed by DAS 2.0 program.The bioequivalence of the two preparations was evaluated.RESULTS:The pharmacokinetic parameters of esomeprazole capsules and esomeprazole tablets were as follows:Cmax were(884.71?265.97)ng?mL-1 and(919.53?218.44)ng?mL-1;tmax were(1.45?0.39)h and(1.65?0.45)h;t1/2 were(2.30?0.51)h and(2.23?0.47)h;AUC0～12 were(3 897.50?1 033.58)ng?h?mL-1 and(4 059.57?1 715.07)ng?h?mL-1;AUC0～∞ were(4 222.70?1 066.73)ng?h?mL-1 and(4 320.22?1 426.73)ng?h?mL-1.The relative bioavailability of esomeprazole capsules was(96.01 ?10.5)%.CONCLUSION:Esomeprazole capsules and esomeprazole tablets are bioequivalent.

OBJECTIVE:To observe the effects of Lingqi capsule(LQC) on the proliferation of human pulmonary adenocarcinoma A549 cells in vitro and the expression of apoptosis related genes.METHODS: The inhibition rates of A549 cells treated with LQC-contained serum were detected with MTT assay.And RT-PCR was used to measure the expression on mRNA of apoptosis related genes-bcl-2 and bax of A549 cells.The morphological change of A549 cells in vitro were observed with Wright-Giemsa staining.RESULTS: The LQC-contained serum in various concentrations could inhibit the proliferation of A549 cells in a dose-dependent manner.The results of RT-PCR showed that LQC-contained serum could down-regulate the expression of bcl-2 gene but up-regulate the expression of bax gene.LQC-contained serum also could change the morphological structure of A549 cells.CONCLUSION: LQC-contained serum has a regulatory effect on apoptosis related genes,which might be one of the main mechanisms of its anti-tumor biological effects.