Objective: To investigate the cytotoxicity and antiviral activity of methanolic extract of S.grandiflora flowers using different cell lines and viruses. Methods: The methanolic flower extracts were prepared and evaluated for their antiviral and cytotoxic activities using viruses like herpes simplex-1 and 2, vaccinia, vesicular stomatitis, cox sackie, respiratory syncytical, feline corona, feline herpes, para influenza, reo-1, sindbis and punta toro viruses in different cell lines, like Hel, HeLa, Crandell Reus feline kidney and Vero cell cultures. Results: Among the viruses used the extract possessed strongest antiviral activity against herpes simplex 1 and 2, repiratory syncytical, para influenza, reo, sindbis, cox sackie and punta toro viruses that was (EC50=20 μg/mL and 45 μg/mL) and moderate activity for remaining viruses (EC50= 100 μg/mL). The antiviral activities assessed by calculating the selectivity index may be due to the presence of flavonoids in the extracts there by inhibit the virus cell fusion in the early and replication stages. The cytotoxicity effect was evaluated using MTT assay and the results revealed that the extracts exhibited cytotoxicity from the range of 20 to 100 μg/mL. Conclusions: Present results confirmed that the S. grandiflora used as a good antimicrobial agent in future.

Introduction: Sleep deprivation is a concern in young adults and the use of mobile phone is very common in this population. This study aimed to measure and correlate the sleep quality, pattern of sleep and problematic mobile phone use in young medical students. Methods: A cross-sectional study was conducted using the Pittsburgh Sleep Quality Index (PSQI), Problematic Mobile Phone Use Scale (PMPUS) and Morningness-Eveningness Questionnaire (MEQ) among medical students in a Malaysian medical college. Correlation among the variables was done using bi-variate analysis followed by Pearson correlation coefficient and 2-tailed significance. A level for p ≤ 0.05 was considered as statistically significant. Results: We found significant positive correlation between poor sleep quality and total problematic mobile phone use as well as its sub dimensions, namely deprivation, adverse outcomes and control disorder. PSQI score showed significant negative correlation with different patterns of MEQ like moderate evening, late sleepers, moderate morning and early risers. Positive correlation of the PSQI with sub dimension of PMPUS reflects unhealthy dependency on mobile phone in this population. Conclusion: Our study revealed that the unhealthy use of mobile phone adversely affected sleep quality in the cohort of young medical students. It is thus important for medical schools to formulate policies and create educational programs vis-a-vis sleep health and to increase awareness regarding controlled mobile phone usage.