Objective To evaluate the curative effect in treatment of hepatic metastases tumor with poorly blood supply through superselective transcatheter nutrient arterial full chemoembolization.Methods 75 cases of hepatic metastases tumors with poorly blood supply were treated by full chemoembolization through superselective transcatheter nutrient arterial infusion under DSA guided.Results After treatment 2～3 months,CT scans showed that totally 202 tumors in 75 cases,the tumors were fully filled with lipiodol in 189,large part in 11 and not well in 2.159 tumors were obviously shrunk,41 tumors were shrunk part,2 tumors were not obviously shrunk.The survival rate in 6 months,12 months,24 months,36 months and 3～5 years was 100%(75/75),85.3%(64/75),55%(41/75),42.7%(32/75)and 20%(15/75)respectively.Conclusion The transcatheter superselective nutrient arterial full filling chemoembolization for hepatic metastases tumor with poorly blood supply is a effective therapy.

Objective To investigate the expression of epidermal growth factor receptor (EGFR) and multidrug resistance protein, and it's relationship with efficacy of chemotherapy in NSCLC.MethodsThe immunohistochemical (EnVision method) was used to detect the expression of EGFR and LRP, P-gp, MRP, GST-π in 95 cases of NSCLC tissues and 15 cases of normal lung samples.ResultsEGFR in NSCLC tissues was significantly correlated with the history of smoking, pathological types of cancer(squamous cell carcinoma vs adenocarcinoma), presence of lymph node metastasis, and TNM stages (P< 0.05).The positive expression rate of LRP and MRP in adenocarcinoma were higher than squamous cell Carcinoma (P< 0.05), and MRP in high differentiate cancer was higher than low differentiate cancer (P< 0.05).The expression of MRP and LRP, PGP and GST-π in NSCLC were positive correlation(r=0.341, P=0.001;r=0.213, P=0.038), there is no correlation between EGFR with LRP, P-gp, MRP, GST-π.EGFR-negative chemotherapy efficiency (62.5%) was significantly higher than EGFR-positive(37.5%, P< 0.05).ConclusionEGFR and multi drug resistance proteins are overexressed in NSCLC tissues.multi drug resistance proteins have a synergistic to drug resistance.EGFR could be used to objectively predict chemotherapy effect and prognosis of NSCLC.

Infantile spasms constitutes an unique,age-specific epilepsy syndrome of early infancy characterized by epileptic spasms often accompanied by neurodevelopmental regression and an electroencephalograph finding of hypsarrhythmia.For the complex and various etiologies,the pathogenesis remains unclear.Although the relevant guidelines and consensuses are being updated all the time,but proven therapies are still limited because of efficacy,tolerability,at times even availability.In this paper,the current findings regarding up-to-date advances in the treatment of infant spasms were reviewed,especially in use of the corticosteroid and vigabatrin as well as the quality-of-care indicators for infantile spasms.

Objective To study the expression of HOXB9 gene in Hela cells,Mocoy cells, SP2/0 cells and U251 cells. Methods The expression of HOXB9 gene was detected by semi-quantitative RT-PCR method. Results Hela cell and U251 cell expressed HOXB9 gene, which SP2/0 cell and Mocoy cell didn't express it. Conclusion The expression of HOXB9 gene was different in different cells.

Objective To investigate the multi-phase dynamic spiral CT findings of small hepatic hemangioma with arterioportal shunts,and to discuss its mechanism and influencing factors.Methods Multi-phase dynamic contrast-enhancement CT findings of 52 small hepatic hemangiomas in 33 cases were analyzed retrospectively.The criteria for diagnosing arterioportal shunts were as follow:①Transient wedge-shape or irregularly shape homogenous enhancement in the liver parenchyma adjacent to the tumor on hepatic arterial phase and slight hyper-or iso-attenuation in the area on portal venous phase and delayed phase;②Some branches of portal vein showed early appearance within the wedge-shaped homogenously hyper-attenuating area on the arterial phase.The correlation between the enhancement patterns of homangiomas and the prevalence of arterioportal shunts in small hepatic homangiomas were analyzed.Results Arterioportal shunts were found in 13(25%)of 52 small hepatic hemangiomas,all of them showed transient wedge-shaped enhancement peripheral to the tumor and in 7 of 13 hemangiomas showed early enhancement of branches of the portal veins on the arterial phase.Eleven(52.4%)of the 21 small hepatic hemangiomas with rapid enhancement had arterioportal shunt shunts,whereas only 2(6.5%)of the 31 hemangiomas with slow enhancement had arterioportal shunt shunts(P

Objective To observe the effect of cinobufotalin freeze-dry powder on heart rate ( HR ) and electrocardiogram ( ECG) of SD rats and to provide experimental basis for monitoring its adverse effect on heart in clinical application. Methods The drug was administered into external jugular vein at constant speed throughout the whole experiment;standard-Ⅱ limb lead monitored the HR and ECG, and then the changes in HR and ECG before and after administration of cinobufotalin were compared. Results Thirty minutes after administration of cinobufotalin injection and cinobufotalin freeze-dry powder at middle dose and high dose, HR of the rats was significantly increased as compared with blank control group[(469±40) bpm, (466±29) bpm and (484±40) bpm vs. (411±17) bpm] (P<0. 05), but soon afterwards, it returned to normal. A small number of rats also developed arrhythmia. In addition, administration of cinobufotalin significantly shortened the P-R interval[blank control group: (46. 90±3. 90) ms, cinobufotalin injection administration group: (39. 70± 2. 54) ms;middle dose of cnobufotalin freeze-dry powder administration group: (37. 70±3. 77) ms; high dose of cinobufotalin freeze-dry powder administration group:(39. 30±7. 12) ms] and Q-T interval[blank control group:(61. 29±9. 46) ms;middle dose of cinobufotalin freeze-dry powder administration group: (55. 13±4. 67) ms; high dose of cinobufotalin freeze-dry powder administration group:(51.75±11. 53) ms] (P<0. 05), but QRS and S-T were unchanged (P>0. 05). Conclusion Cinobufotalin freeze-dry powder has some side effects on rat heart and can increase HR, even lead to arrhythmia.

Objective: To explore the effect of LBP X on apoptosis of human leukemia HL 60 cells. Methods: The inhibitory effect of LBP X on growth of HL 60 cells was assayed by MTT method. The membrance fludity was determined with DPH fluorigenic labeling technique. HL 60 cells were stained with Hoechst 33 258 for nuclear morphology analysis. Agarose gel electrophoresis and flow cytometry were used to analyze apoptosis qualitatively and quantitatively. Results: 20-1 000mg/L LBP X inhibited the growth of HL 60 cells in dose dependent manner and the membrance fludity decreased. The nuclei of HL 60 cells treated with LBP X for 48 h shrinked, condensed and cleaved. Agarose gel electrophoresis of DNA from cells treated with LBP X revealed "DNA ladder". HL 60 cells exposed to LBP X showed apoptotic peaks. Conclusion: LBP X can induce apoptosis in human leukemia HL 60 cells.

Electroencephalography (EEG) is an important instrument for the evaluation of brain function, and an irreplaceable diagnostic technique for nervous system diseases. At present, China still lacks professional child-EEG talents. Therefore, it is a task of great priority to establish an effective and practical training method and foster more child-EEG physicians. As most trainees have not learned EEG before and only have limited time for learning, we divide the child-EEG training into three phases, includ-ing theory learning, practice training, and EEG reading and interpretation on the basis of the general rules in learning EEG. In the theory learning phase, basic EEG knowledge is taught comprehensively to form a solid foundation for future study. In practice training phase, the trainees acquire important skills of EEG by carrying out complete EEG monitoring, eliminating EEG artifacts, observing seizures, and read real-time EEG. In the phase of EEG reading and interpretation, the trainees learn to analyze EEG gradually by read-ing and report EEG under the guidance of the senior physician. Strict examination is arranged for each phase to evaluate study results objectively. The phased model is designed to implement a step-by-step training of child-EEG and foster the trainee's independent ability to carry out EEG inspection.

Objective To investigate the value of MRI in the diagnosis and differential diagnosis of bladder leiomyoma.Methods Clinical data,pathological files and MRI findings of 8 patients with bladder leiomyoma that verified by pathologic were analyzed retrospectively.Results 4 cases of bladder leiomyoma were located in front bladder wall, 3 cases were in post bladder wall and 1 case in right bladder wall;meanwhile, 6 cases were endovesical(75.0%),1 case was intramural(12.5%) and another 1 case was extravesical(12.5%).All lesions displayed equal signals on T1WI, equal or slightly high signals on T2WI with mild and moderate enhancements;bladder wall adjacent to tumors and structures around were not invaded by tumors.6 cases of all displayed inhomogeneous signals on T2WI and with some patchy or stripe high signals in it, the signals turned to equal on a fat-saturated T1WI which was similar with muscle of pelvic wall.Conclusion MRI features of bladder leiomyoma showed certain characteristics.

[Objective] To compare the disinfection effect of different disinfectants on disinfection effect of laryngeal endoscope,and seek for a more safe and effective disinfectant for laryngeal endoscope.[Methods] 180 laryngeal endoscope were divided into three groups with 60 of them in each group.Acidic electrolyzed oxidizing water(AEOW),2％ glutaraldehyde and orthophthalaldehyde were used for disinfection.Three minutes and five minutes after disinfection,the samples were got and underwent bacterial detection.The disinfection effect after three minutes and five minutes was compared.[Results] The qualified rate of disinfection and cost between AEOW,2％ glutaraldehyde and orthophthalaldehyde showed distinct difference.[Conclusions] Using acidic electrolyzed oxidizing water in the disinfection of laryngeal endoscope has advantages of fast,effective,low-cost,which can be applied in the clinical work.