1.Effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-?
Chinese Traditional and Herbal Drugs 1994;0(09):-
Objective To investigate the effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-?. Methods Flow cytometry was used to analyze the apoptosis. The caspase-8 fluorescent kits were used to detect the activity of caspase-8. And the expression of caspase-8 enzymogen was examined by Western blot. Results Procyanidin (5, 10, and 20 mg/L) significantly inhibited the apoptosis of HeLa cells induced by rhTNF-? (100 ?g/L) (P
2.Effects of polyporus polysaccharide on activity and mRNA expression of inducible nitric oxide synthase in peritoneal macrophages of mice
Dinan HUANG ; Gan HOU ; Qifeng ZHU
Journal of Integrative Medicine 2004;2(5):350-2
OBJECTIVE: To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS). METHODS: The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively. RESULTS: PPS could elevate the iNOS activity with dose-dependence and stimulate the iNOS mRNA expression of peritoneal macrophages in mice. CONCLUSION: The regulation of PPS on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS. This indicates that the mechanism of PPS's antitumor and immunoregulation functions may be related to increasing NO output of macrophages through stimulating iNOS's denovo synthesis.
3.A Trial Practice of Blended Learning Course with MOOCs′Resources---Taking“Doctor-Patient Communication” as an Example
Shengtian HOU ; Yonghe GAN ; Haixing WANG
Chinese Medical Ethics 2015;(5):815-817
The emergence of Massive Open Online Courses ( MOOCs ) has provided enormous resources to blended learning and flipped classroom .By taking “Doctor-Patient Communication” as an example , this article discusses how to utilize MOOCs , online resources and popular technology platforms to construct a new -type teach-ing mode based on blended learning .The study found that MOOCs meet students′diverse learning needs , improve the students′ability to solve problems , improve the students′learning autonomy , changing the traditional way of ex-amination and evaluation , promoting the improvement of the teachers and students comprehensive quality , also im-prove the teaching effect , promote the teaching reform and practice .
4.Present situation of using health information technologies in residents of Beijing
Shengtian HOU ; Yonghe GAN ; Qiyao BAI
Chinese Journal of Medical Library and Information Science 2016;25(4):41-44
Objective To provide reference for promoting the development of health information technologies by sur-veying the present situation of using health information technologies in residents of Beijing. Methods The present situation of using health information technologies in residents of Beijing was investigated using self-designed ques-tionnaires. A total of 584 valid questionnaires were returned. Results Of the 584 visited residents of Beijing, 67. 3%searched the online health information, 47. 6% considered that the online health information was reliable, 49. 5%had their appointment with a doctor, 485% used APP to search health information, 67. 0% believed that such a software was useful in seeking for doctors and drugs, 71. 4% showed their wishes of online shopping prescription drugs, and 75. 7% were ready to visit online hospitals. Conclusion The rate of searching online health information is rather high with a low reliability in the residents. The trend to have an appointment with a doctor is good in the residents. The APP has a great potential in searching online health information. The acceptability of online shop-ping prescription drugs and visiting online hospitals is high.
5.The Promotion of Setting up Analysis Technique of Molecular Biology on the Experimental Skills in Laboratory Medicine
Ailing LIANG ; Yongjun LIU ; Gan HOU
Chinese Journal of Medical Education Research 2003;0(03):-
To adapt the developing requirement of clinical laboratory diagnostics,the Analysis Technique of Molecular Biology was set up for Grade 2003 in laboratory medicine in our college.The students showed great interests and enthusiasm in this subject,especially in laboratory course.The studentsof Grade 2003 made a better score than those of Grade 2002 in the final Test of Basic Experimental Skills,which included the scores of basic operation,experiment theory and technical skill.These results showed that setting up the Analysis Technique of Molecular Biology could improve student's ability in experimental skills.
6.Advantages and Disadvantages of Bilingual Teaching in Medical Biochemistry
Dinan HUANG ; Gan HOU ; Qifeng ZHU
Chinese Journal of Medical Education Research 2002;0(01):-
Medical biochemistry is a frontal science, esp. molecular biology. The bilingual teaching in medical biochemistry has not only advantages but also disadvantages. By making use of its advantages and correcting its shortcomings, we should try our best to be close to international level in the teaching of medical biochemistry.
7.Effects of lentinan on nitric oxide production and intracellular glutathione in mouse peritoneal macrophages
Gan HOU ; Dinan HUANG ; Qifeng ZHU ;
Chinese Traditional and Herbal Drugs 1994;0(03):-
Object To investigate the mechanisms of action of lentinan (LTN) activated mouse peritoneal macrophages to produce nitric oxide (NO). Methods The effects of LTN on NO output and intracellular glutathione (GSH) in mouse peritoneal macrophages and the correlation of them were studied. Results (1) LTN can significantly increase production of NO in mouse peritoneal macrophages, decrease level of intracellular GSH followed increase of NO production. (2) The above effect can be blocked efficiently by the NO production inhibitors. (3) NO production can be inhibited by GSH lowering drugs. Conclusion LTN increase NO production with depletion of intracellular GSH in the activated mouse peritoneal macrophages. It suggested that intracellular GSH plays an important role in regulation of NO production and protection of host cells from cytotoxic attack induced by NO.
8.Effect of inhibition of hPOT1 expression mediated by siRNA on Caspase-3 in HeLa cells
Gan HOU ; Dinan HUANG ; Yinghua JIANG ; Ailing LIANG
Chinese Pharmacological Bulletin 1987;0(02):-
Aim To inhibit the expression of human protection of telomere gene hPOT1 in HeLa cells using vector based on RNA interference(RNAi) technique and to investigate its effects on the activation of Caspase-3 in HeLa cell.Methods Three recombinant plasmids(our group constructed before) containing different hPOT1 target sequences(pmU6-shRNA1,pmU6-shRNA2 and pmU6-shRNA3)were transfected into HeLa cells by liposomal.The expression inhibition of hPOT1 was detected by RT-PCR and EMSA.The expression and activation of Caspase-3 were inspected by RT-PCR,Western blot analysis and Caspase-3 kit.Results After 48 hour′s transfecting,hPOT1 mRNA and protein level in HeLa cells reduced and the expression of Caspase-3 mRNA had no change.Caspase-3 zymogen decreased but its activity increased.Conclusions hPOT1 expression in HeLa cells can be significantly inhibited by using siRNAvectored RNAi and the down-regulation of hPOT1 expression can activate Caspase-3.
9.Promotion of resveratrol on psoriasis like skin damage by down-regulating expression of Keratin 17
Ming WEI ; Lu GAN ; Jin HOU ; Lihong CHEN ; Yantong LIU
Drug Evaluation Research 2017;40(1):37-41
Objective To observe the therapeutic effect of resveratrol (Rsv) on psoriasis like skin damage induced by imiquimod (IMQ) and study its mechanism.Methods BALB/c mice were randomly divided into four groups:control group,Rsv group,model (IMQ) group and Rsv treatment (IMQ + Rsv) group with six mice in each group.Psoriasis like skin model induced by smearing IMQ in the mice's ear for 8 d,20 mg Rsv cream were gave at the same time for treatment.Observe erythema and psoriasis degree,and measure ear thickness with vemier caliper.HE staining was used to observe tissue change,real time quantitative PCR (qRT-PCR) was used to detect K17,IL-17A,IL-19 and IL-23 mRNA and Western blotting were used to detected K17 protein expression in tissues.Results Compared with the model group,mice erythema area and the thickness of epidermal scale decreased significantly in Rsv treatment group;HE staining results showed that,Rsv significantly reduced ear marginal tissue epidermal thickness,and diminished severity of the psoriasis-like skin inflammation.qRT-PCR and Western blotting confirmed a Rsv dependent decrease of in K17,IL-17A and IL-19 in mRNA level and protein levels of K17.Conclusion Rsv improves psoriasis like skin damage by down-regulating expression of Keratinl 7.
10.Spindle cell carcinoma of the breast:a clinicopathologic analysis of three cases
Zhongyan GUAN ; Yufang FENG ; Zhenkang GAN ; Liang SHI ; Yingyong HOU
Chinese Journal of Clinical and Experimental Pathology 2015;(9):996-1000
Purpose To analyze the clinical pathological characteristics and pathological diagnosis and prognosis of spindle cell carci-noma of the breast. Methods Three cases of spindle cell carcinoma of the breast were studied by morphological and immunohisto-chemical EnVision techniques. Results The females were 48, 63 and 71 years old. The tumors located in the right breast with 4. 0 cm × 3. 0 cm × 3. 0 cm, 3. 0 cm × 2. 0 cm × 2. 0 cm and 3. 5 cm × 2. 8 cm × 2. 3 cm in size and showed cystic lesion. The neoplasm was composed of bland spindle cells and mimicking fibromatosis. Immunohistochemical staining showed that spindle cells were positive for CK(AE1/AE3), CK(34βE12), CK14, CK5/6, p63 and vimentin, negative for ER, PR and c-erbB-2. Ki-67 was positive in 20%, 25% and 20% of the cells. Conclusion Spindle cell carcinoma of the breast is a rare subtype of the metaplastic carcinoma which tend to show cystic changes. It is important to make a definite diagnosis which combine histopathologic features and immunophe-notyping.