1.Yacon Extract Attenuated Kidney Fibrosis in 5/6-subtotal Nephrectomy Mouse Model by Upregulating HGF and BMP-7 mRNA Expression
Setyo Purwono ; Abdur Rahman Faqih Al Jundi ; I Made Kawiyasa ; Alfina Meidina Rizka Cahyatika ; Andika Putra Cipta ; Galih Asa Andrianto ; Rendy Candra ; Dwi Cahyani Ratna Sari ; Fajar Dwi Astarini ; Eryna Ayu ; Nugra Desita ; Nur Arfian
Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 3,June):94-100
Introduction: Chronic kidney disease (CKD) leads to tubular injury, kidney fibrosis and anemia. These conditions
are influenced by fibrotic and anti-fibrotic substances, such as Transforming Growth Factor beta-1 (TGF-β1), Hepatic
Growth Factor (HGF), and Bone Morphogenic Protein-7 (BMP-7). Yacon is an herbal medicine which has not been
elucidated in CKD. This study aimed to investigate the effect of ethanolic extract of Yacon leaves on attenuating
renal injury in CKD model in mice. Methods: We performed 5/6 subtotal nephrectomy (SN) in male Swiss-Webster
mice (3 months old, 30–40 grams) to induce chronic kidney disease, then the mice were sacrificed at day 14. The
mice (n=25) were divided into five groups: one SN group, three groups of SN with administration of Yacon extract,
and one group of sham operation (SO, with supplementation of 0.1% aquadest). There were three different doses of
ethanolic extract of Yacon leaves: 98 mg/kg BW (SN+YK1), 49 mg/kg BW (SN+YK2), and 24.5 BW mg/kg (SN+YK3).
Tubular injury, perivascular and interstitial fibrosis were quantified based on histopathological examination. Reverse-transcriptase PCR (RT-PCR) was performed to quantify HGF and BMP-7. Results: SN group demonstrated CKD
with elevation of creatinine level, anemia, tubular injury, glomerulosclerosis, and fibrosis. Yacon extract treatment
showed attenuation of injury with lower creatinine level, tubular injury, glomerulosclerosis and fibrosis compared
to the SN group. HGF and BMP-7 mRNA expressions were higher in Yacon-treated groups than the SN group. Conclusion: Yacon treatment might ameliorate CKD through reducing fibrosis and increasing expression of anti-fibrotic
genes.