0.05).Conclusion The SDF-1/CXCR4 protein may play a role in the malignant transformation of primary ovarian cancer,and it may participate in the initiation,progression and metastasis of ovarian cancer.

Objective: To study the anti-senile and anti-oxidative activities of Forsythia suspense leaves tea(FSLT) in mice. Methods: 36 ICR mice(7 mo age) were randomly divided into three groups: senior control group,ig distilled water ; FSLT1 group,ig FSLT 0.3 g ?kg-1?d-1 ; and FSLT2 group,ig FSLT 0.9 g?kg-1?d-1 .The other 12 ICR mice(young control group) were given ig distilled water. MDA and MAO-B in brain and mitochondria were measured. SOD,MDA and POD in heart, liver index, thymus index and spleen index were determined. Results: Forsythia suspense leaves tea may reduce the level of MDA and MAO-B, and increase the activities of SOD and POD. Conclusion: Forsythia suspense leaves tea may have anti-senile and anti-oxidative activities.

Objective:To explore the molecular mechanism of Guchang-Zhixie pill in the treatment of ulcerative colitis based on network pharmacology. Methods:Retrieve the TCMSP database to get the effective components and target genes of each drug in Guchang-Zhixie pill, and then retrieve the OMIM database Disgenet database to get disease genes, and then intersect the drug genes and disease genes to get the core genes. Used STRING database to build gene function association network, and used DAVID database to analize go enrichment and pathway enrichment of the core genes. Results:A total of 77 active ingredients and 211 targets of Guchang-Zhixie pill were obtained by TCMSP database, 914 genes of ulcerative colitis were obtained by retrieving disease gene database, 72 core genes were obtained by intersection of drug gene and disease gene. Topology analysis showed that the core targets were IL6, IL1B, MAPK1, VEGFA, MMP9, etc; Twelve enriched biological process clusters were obtained. The biological processes with more contact targets were positive regulation of RNA polymerase Ⅱ promoter transcription and positive regulation of DNA template transcription; A total of 14 enriched pathway clusters were obtained by pathway enrichment analysis, among which TNF signaling pathway and PI3K/Akt signaling pathway were closely related to inflammation and associated with more targets. Conclusion:The target and pathway of Guchang-Zhixie pill in the treatment of ulcerative colitis are preliminarily obtained through database analysis, which has provided the reference for clarifying its mechanism.

AIM: To investigate the role of post-hemorrhagic shock mesenteric lymph (PHSML) in the enhancementof vascular permeability .METHODS: Eighteen Wistar rats were randomized into sham group , shock group,and shock plus mesenteric lymph drainage (shock +drainage) group.The rats in shock group and shock +drainagegroup were routinely subjected to hemorrhagic shock and hypotension [(40 ±2) mmHg] was maintained for 90 min, andthen the fluid resuscitation was performed.Mesenteric lymph was drained in the rats in shock +drainage group from resuscitationfinished to 6 h, for the observation of PHSML drainage on the vascular permeability in multiple tissues of hemorrhagicshock rats.Afterwards, human umbilical vein endothelial cells (HUVECs) were incubated with the PHSML in vitro to observethe effects of PHSML on the morphology and permeability of HUVECs .RESULTS: The degree of blue color and concentrationsof Evens blue in the lung, myocardium, kidney, liver, spleen and small intestine were significantly increased inthe shocked rats than that in sham group, while the ratios of the dry weight to the wet weight were decreased .The mesentericlymph drainage reversed these changes .Meanwhile, 4% and 10% of PHSML at 0 ~3 h and 3 ~6 h after resuscitation,and lipopolysaccharide (10 mg/L) all caused the damage of HUVECs, decreased the viability and trans-endothelial electricalresistance of HUVECs, and increased the permeability of HUVECs to fluorescein isothiocyanate -labeled albumin. CONCLUSION: PHSML is a vital factor in the enhancement of vascular permeability .

Objective This study is aimed to investigate the prognostic significance of ring sideroblast ( RS) in MDS( Myelodysplastic Sydrome ) and evaluate the correlation of RS and other prognostic index.Methods A total of 198 patients with MDS between March 2009 and December 2015 in Chinese PLA′s Gerneral hospital were chosen for this study .Based on the ratio of RS in nucleated red blood cell , patients were first separated into myelodysplastic syndrome without ring sideroblast (MDS RS-) group, RS≥15%, and myelodysplastic syndrome with ring sideroblast ( MDS RS +) group, RS <15%. Then, according to the proportion of blasts in bone marrow nucleated cells above 5%or below, patients were further divided into myelodysplastic syndrome with low blasts without ring sideroblast ( MDS-LB RS-) group, myelodysplastic syndrome with low blasts and ring sideroblast ( MDS-LB RS+) group, refractory anemia with excess blast without ring sideroblast ( RAEB RS-) group and refractory anemia with excess blast and ring sideroblast ( RAEB RS+) groupe.All patients had completed the morphological , genetics , molecular biology examination at dignosis, and followed up by phone.The results of the overall survival (OS) analysis have been presented in a Kaplan-Meier curve and cox regression model .Last, according to the percentage of RS in nucleated red blood cell , patients were separated into RS <5%groupe, 5%-15%group, 15%-40%group, RS≥40%group, and analyse their survival prognosis by statistical methods .Results Comparing to MDS RS-group, the morbidity age, WBC and PLT count were significantly higher [61 ±1.91 vs 52 ±1.37, t=-3.555, P<0.01, 3.82(0.47-323)vs 2.6(0.6-59.7), z=-4.014, P<0.01;139.5(7-608) vs 60(3-724), z =-3.988, P<0.01], bone marrow eythroid hyperplasia and gigantocyte were more obvious in MDS RS+group[χ2 =11.032, P<0.01, χ2 =5.165, P<0.05]; the percentage of GATA1 gene and abnormal rate of poor prognosis gene ( MLL, NRAS, WT1 ) , either mutation or high gene expression , were higher in MDS-LB RS+group than that in MDS-LB RS-( P<0.05 ); Contrasting with RAEB RS-group, the karyotype is worse in RAEB RS +group[χ2 =4.966, P<0.05];Comparing to 15%-40%group, the OS were poorer in RS≥40%;MDS RS+patients were more prone to adverse prognosis than MDS RS-patients.Conclusion Compared to MDS RS-group, MDS RS +patients had worse prognosis;RS maybe correlate to morbidity age , eythroid dysplasia and gene abnormality in affecting the survival prognosis of MDS.

Objective:To explore the mechanism of Cordyceps sinensis on chronic obstructive pulmonary disease (COPD) by means of network pharmacology.Methods:By searching in TCMSP database, extracted the active ingredients and targets of Cordyceps sinensis; by searching in GeneCards and OMIM database, extracted the disease targets of chronic obstructive pulmonary disease (COPD) , then constructed the drug-disease and protein interaction network, and analyzed GO enrichment and KEGG on the targets.Results:Seven effective components and 84 effective targets of Cordyceps sinensis were obtained from TCMSP database, 5 142 COPD-related genes were obtained from GeneCards and OMIM database, 31 key targets for Prunella vulgaris to treat non-small cell lung cancer were obtained from drug-disease network, and 31 PPI networks were established, with 31 nodes, 77 edges and rich GO. GO enrichment found that the moderate targets include CASP3, NOS3, PPARG and RELA. KEGG enrichment yields 64 biological processes involving 17 signaling pathways.Conclusions:This study preliminarily validates the role and mechanism of Cordyceps sinensis in the treatment of COPD, and provides a reference for further research on the selection of target genes and signal pathways.

This study deeply analyzes the common problems of three military medical universities in the management of undergraduate extracurricular scientific research, such as lack of communication means, limited online resources, backward laboratory opening and low utilization rate of equipment. We have built a cloud platform management system for undergraduate extracurricular scientific research. This system firstly sets up a teaching resources storage module including videos, PPTs, documents, pictures, electronic materials, question bank, etc. Then four subsystems for different roles of students, mentors, experimental teaching staff and administrators are constructed. Finally, this system realizes independent experiments by students, real-time evaluation by mentors, instrument sharing and efficient management through the seamless connection with the user terminal equipment. And the study also makes evaluation on the present usage.

【Objective】 To explore the correlation of preoperative fibrinogen-to-albumin ratio （FAR） with the clinicopathological characteristics and prognosis of patients with rectal cancer so as to clarify the role of coagulation function and nutritional status in the occurrence and progression of tumors. 【Methods】 We retrospectively analyzed the clinicopathological data of 647 patients with rectal cancer who underwent radical resection in The First Affiliated Hospital of Xi’an Jiaotong University from January 1， 2013 to December 31， 2016. According to the optimal cut-off point value of FAR determined by receiver operating characteristic curve， 647 rectal cancer patients were divided into high FAR level group and low FAR level group. The correlation between different preoperative FAR levels and clinicopathological characteristics of rectal cancer patients was analyzed. Multivariate Cox regression analysis was used to analyze the independent risk factors for the prognosis of rectal cancer patients. R software was used to construct the nomogram， and C index and calibration chart were used to evaluate the prediction efficiency of the nomogram. 【Results】 Preoperative FAR levels had a good predictive value for the prognosis of rectal cancer patients. The area under ROC curve was 0.771， the optimal cut-off point was 0.092， and the Youden index was 0.446. There were statistically significant differences in age， T stage， N stage， TNM stage， preoperative CEA levels and preoperative CA19-9 levels between rectal cancer patients with different preoperative FAR levels （P<0.05）. The overrall survival and disease-free survival of rectal cancer patients with different preoperative FAR levels had statistically significant differences （P<0.05）. In the multivariate analysis， preoperative FAR levels （≥0.092， HR=3.298， 95% CI： 2.365―4.600， P<0.001）， age （≥60 years， HR=2.110， 95% CI： 1.479―3.012， P<0.001）， TNM stage （Ⅲ grade， HR=6.743， 95% CI： 2.771―16.771， P<0.001）， grade of differentiation （poor， HR=1.639， 95% CI： 1.104―2.432，P=0.014）， preoperative CA19-9 levels （≥37 U/mL， HR=2.205， 95% CI： 1.529―3.180， P<0.001） and not perform postoperative chemoradiotherapy（HR=1.792， 95% CI： 1.294―2.480，P<0.001） were independent risk factors of overall survival for patients with rectal cancer. OS and DFS nomograms of rectal cancer were established by the Rlanguage software， and the C-index was （0.781， 95% CI： 0.749―0.815； 0.754， 95% CI： 0.693―0.760）， respectively. The calibration curve of the nomogram showed high consistence between predictions and actual results for 1-year， 3-year， 5-year OS and DFS. 【Conclusion】 The preoperative high FAR level was an independent risk factor for the prognosis of patients with rectal cancer. It can be supplemented with pathological factors such as TNM stage as prognostic indicators for patients with rectal cancer， which may be helpful for clinicians to follow up or make beneficial treatment for rectal cancer patients.