Objective To explore the feasibility of two-port laparoscopic drainage in the treatment of liver abscess. Methods A total of 18 patients with bacterial hepatic abscess underwent twoport laparoscopic drainage.Two incisions were made at the superior border of the umbilicus and 4～5 cm below the right costal margin near the anterior axillary line,respectively.The abscess was cut through in "┼" or "╫" shape.The pus cavity was debrided bluntly,and a double-lumen drainage tube was placed.Results The laparoscopic drainage was performed successfully in all the 18 patients.The operative duration was 30～75 min(mean,45 min).A bile leakage for 80～100 ml per day occurred in 1 patient.The drainage tube was removed on the 12th postoperative day and the patient was discharged from hospital on the 14th postoperative day.Of the 18 patients,the hospital stay after operation was 7～14 d(mean,8.5 d).All the patients were followed for 5～12 months(mean,9 months) and no residual abscess or recurrence was found. Conclusions Two-port laparoscopic drainage for liver abscess is an ideal approach with advantages of simple manipulation,slight contamination inside abdominal cavity,little trauma,low complication rates,and rapid recovery.

AIM: To investigated the changes of hippocampus noradrenaline levels and its tyrosine hydroxyase (TH) , monoamine oxidase A (MAOA) expressions during chronic psycological stress procedure and physical exercise in rats. METHODS: Male Wistar rats (n =24) were randomly assigned to three conditions; (1) restraint stress (S) at 6 h/d for 3 weeks; (2) 24 h access to activity wheel running (E) for 4 weeks; (3) a sedentary control group (C). Levels of NE were assayed by high performance liquid chromatography with electrochemical detection. Protein expression of TH and MAOA were detected by Western blotting. RESULTS: Compared to C, weight loss and sucrose consumption rate decrease were induced in S, and NE was lower while TH and MAOA expressions increased significantly. In E group, NE was higher and TH, MAOA increased distinctly. CONCLUSION: It is suggested that hippocampus NE level may play an important role in the mechanism on the protection of physical exercise to chronic psycological stress, there is no close connection between NE change and TH, MAOA.Other mechanisms may exist.

AIM: This study is to determine changes of hippocampal norepinephrine (NE) and serotonin (5 -HT) in long term physical exercise and chronic psychological stress, and to study the roles of the two monoamine transmitters in the effect of exercise counteracting stress - induced hippocampal damages in brain. METHODS : Levels of hippocampal NE and 5 - HT in rats undergoing 4 - week voluntary wheel running exercise (exercise group) or 3 - week restraint stress (stress group) or 4 - week exercise and 3 - week stress (exercise - stress group) were detected by high - performance liquid chromatography using electrochemical detection. RESULTS: It is showed that levels of hippocampal NEand 5 - HT increased significantly (P < 0. 01) in the exercised rats, and in the stressed rats, hippocampal 5 - HT levelssignificantly decreased(P < 0. 05). Additionally, the NE levels maintained significant high (P < 0. 01) in exercise -stressed rats compared to the pure stressed ones. On the other hand, no obvious difference was observed in hippocampal5 - HT levels between stress group and exercise - stress group, which were all significant lower (P < 0. 05) than that in exercise group. CONCLUSION: It is suggested that both the NE and 5 - HT may play important roles in mediating the exercise-induced positive effects and the 5 - HT may play an important role in stress - induced negative effects on the hippocampus. Moreover, NE may take more action in the exercise attenuating stress - induced hippocampal damages. The hippocampal NE may be more susceptible to exercise, and the hippocampal 5 - HT may be more susceptible to stress.`

This study was aimed to explore the clinical mechanism of combined acupuncture and traditional Chinese medicine (TCM) in the treatment of polycystic ovarian syndrome (PCOS) and to evaluate its clinical curative effect.A total of 100 PCOS patients were randomly divided into three groups,which were group A,B and C,with 50 cases in each group.Group A was treated with ethinyl estradiol and clomiphene.Group B was treated with TCM.Group C was treated with acupuncture combined with TCM.One menstrual cycle was counted as one treatment course.There were a total of 6 treatment courses.Observation was given on menstrual condition of the patient,basal body temperature (BBT),level of sex hormones,ovulation and follicular before and after treatment.The results showed that the total effective rate of group C was 82％,which was significantly higher than that of the control group (P ＜ 0.05).The normal rate of menstruation recovery of group C was 100％,which was significantly higher than those of group A and B (P ＜ 0.05).After treatment,the biphasic rate of BBT in group C was 70％,which was significantly higher than that of the control group (P ＜ 0.05).The basal FSH (14.6±2.9) IU/L and basal E2 (53.2± 12.4) pg/ml of group C were significantly higher than those of group A and B.The basal LH (25.4±5.8) IU/L,T (1.0±0.2) μg/L of group C were significantly lower than those of group A and B (P ＜ 0.05),with statistical significance.It was concluded that acupuncture combined with TCM can be used in the treatment of PCOS by regulating menstruation,adjusting BBT,the level of sex hormone,and promoting ovulation.

This study was aimed to investigate the effect of acupuncture combined with traditional Chinese medicine (TCM) on the incidence of lipid metabolism and the incidence of hyperandrogenism in patients with polycystic ovary syndrome (PCOS).The clinical data of 85 PCOS cases in our hospital from March 2015 to August 2016 were retrospectively analyzed.There were TCM treatment group and acupuncture combined with TCM group according to the treatment method.The difference of blood lipid level,hormone level,incidence rate of hyperandrogenism and clinical symptom score before and after treatment in both groups were observed.The results showed that the incidence rate of hyperandrogenism was 2.22％ in the acupuncture combined with TCM group,which was significantly lower than TCM group (x2=7.070,P =0.008).There was no difference on blood lipid level before treatment in both groups.After treatment,levels of TC,LDL-C and TG of the acupuncture combined with TCM group were significantly lower than those of the TCM group;however,level of HDL-C was higher than that of the TCM group (t =1.817,-3.627,2.554,2.226,P ＜0.05).There was no difference on hormone levels before treatment.After treatment,hormone levels of the acupuncture combined with TCM group were significantly lower than the TCM group.Before treatment,there was no difference on TCM symptom score between both groups.After treatment,the TCM symptom score of the acupuncture combined with TCM group was significantly lower than that of the TCM group.It was concluded that acupuncture combined with TCM had a better therapeutic effect on PCOS.It can improve the blood lipid level of patients and reduce the incidence of hyperandrogenism.It had good clinical value.

The aims of this research were to isolate a Aspergillus niger strain with higher beta-glucosidase activity. We utilized the beta-glucosidase producing strain Aspergillus niger CGMCC 3.316 as the original strain to first obtain a mutant 3-3M through ultraviolet irradiation. Then we studied the conditions of protoplast release and regeneration for strain 3-3M. We treated the protoplasts of strain 3-3M via ultraviolet irradiation and obtained another isolated mutant 60B-3D. The strain 60B-3D showed much higher beta-glucosidase production than the original strain and 3-3M strain. The beta-glucosidase activity of strain 60B-3D was 23.4 IU/mL, with an improvement of 39% compared with the original strain, and 23% compared with strain 3-3M. We also studied the fermentation process of strain 60B-3D, and compared it with the original strain and strain 3-3M. We found the strain 60B-3D exhibited an improvement in xylanase production. The comparison results also showed that the strain 60B-3D secreted more protein. These results were beneficial for producing beta-glucosidase through this productive mutant.
Amino Acid Sequence ; Aspergillus niger ; enzymology ; genetics ; Endo-1,4-beta Xylanases ; biosynthesis ; Fermentation ; Molecular Sequence Data ; Mutagenesis ; Mutation ; Protoplasts ; enzymology ; beta-Glucosidase ; biosynthesis

Objective: To investigate the possibility of heart rate as an optimum index for evaluating physical labor intensity. Methods: From September to October, 2015, a total of 111 male young adults were selected as study subjects. A cycle ergometer was used for exercise at different intensities (20, 40, 60, 80, 100, 120, 140, 160, and 180 W) , and the exercise at each intensity lasted for 6 minutes. The subjects wore the K4b2 portable cardiopulmonary function testing instrument for the real-time monitoring of heart rate (HR) , pulmonary ventilation volume (Ve) , oxygen consumption (VO₂) , and energy metabolic rate (E) . The origin software was used for correlation and regression analyses of HR, Ve, VO₂, and E collected at different intensities to establish Ve-HR, VO₂-HR, and E-HR regression equations. Results: At different intensities, HR was positively correlated with Ve, VO₂, and E (r=0.8985, 0.8733, and 0.8973, all P<0.01) . There was a significant difference in the R² value of Ve-HR, VO₂-HR, and E-HR regression equations (R²=0.8070, 0.7625, and 0.8049, all P <0.01) . The validation results showed that there was no significant difference between the calculated values and the measured ones (P>0.05) . Conclusion HR can replace Ve, VO₂, and E and be used as an optimum index for evaluating physical labor intensity.

Objective:To explore the effect of family function combined with positive suggestion on self-assessment and quality of life of patients with ischemic stroke.Methods:Totally 120 patients with ischemic stroke admitted to hospital from May 2018 to May 2019 were selected by experimental research method, and they were divided into control group and observation group according to the random alphabet, with 60 cases in each, the control group was given positive suggestion, while the observation group was given family function combined positive suggestion, the Activities of Daily Life (ADL), General Self-Efficacy Scale (GSES), Self-rating Depression Scale (SDS), Self-rating Anxiety Scale (SAS), the MOS item short form Health Survey (SF-36) were compared before and after nursing and nursing satisfaction.Results:Before nursing, the ADL scores of the observation group and the control group were (34.61±4.36), (35.58±4.82) points, GSES scores were (17.94±2.48), (17.38±2.62) points, SAS scores were (61.82±6.57), (61.37±6.64) points, SDS scores were (63.71±6.65), (63.28±6.94) points respectively, and there was no significant difference between the two groups ( P> 0.05); after nursing, the ADL score of the observation group was (69.85±7.84) points, higher than (58.39±6.72) points of the control group, the GSES score was (24.25±2.69) points, higher than (20.48±2.31) points of the control group, the SAS score was (47.64±4.91) points, lower than (54.52±5.83) points of the control group, and the SDS score was (50.29±5.26) points,lower than (54.15±5.91) points of the control group ( t values were from 3.779 to 8.597, P< 0.05). There was no significant difference in SF-36 score between the two groups before nursing ( P> 0.05), and the improvement of the observation group was significantly better than that of the control group after nursing ( t values were 2.037-3.656, P< 0.05). The nursing satisfaction of the observation group was 90.00% (54/60), higher than 75.00% (45/60) of the control group ( χ2 value was 4.675, P< 0.05). Conclusion:Family function combined with positive suggestion can significantly improve the life ability and self-efficacy of patients with ischemic stroke, relieve anxiety and depression, significantly improve the quality of life, and obtain satisfactory nursing effect.

Objective To observe the effect of Shenfu injection on lung injury caused by hemor-rhagic shock(HS)in rats and explore the related potential mechanism.Methods Thirty-six SPF healthy male SD rats,aged 16-17 weeks,weighing 400-600 g,were randomly divided into three groups:sham op-eration group(group SH),HS group(group HS),and Shenfu injection group(group SF),12 rats in each group.In group SH,only the right femoral vein and femoral artery were separated after anesthesia,and ve-nous catheterization was not performed.HS model was established in groups SF and HS.In group HS,liquid resuscitation was performed through an intravenous catheter,and the resuscitation fluid consisted of the auto-blood lost and the compound sodium chloride injection equivalent to 1.5 times the blood loss and 10 ml/kg normal saline.In group SF,the resuscitation fluid consisted of the lost autoblood and the compound sodium chloride injection equivalent to 1.5 times the blood loss and Shenfu injection 10 ml/kg.The whole perfusion time was about 60 minutes.Six rats in the three groups were randomly anesthetized 24 and 48 hours after op-eration.The wet/dry weight ratio(W/D)of lung tissues was detected.The concentrations of interleukin-6(IL-6),IL-17,IL-10,and transforming growth factor-β(TGF-β)were detected by ELISA,the mRNA ex-pression of retinoic acid-related orphan nuclear receptor γt(RORγt),transcription factor forkhead box pro-tein 3(Foxp3),and hypoxia-inducible factor-1α(HIF-1α)in lung tissues were detected by PCR.The pro-tein contents of RORγt,Foxp3,HIF-1α,aquaporin 1(AQP1),and AQP5 in lung tissue were detected by Western blot.Pathological changesunder HE staining light microscope and lung injury scores were observed.Results Compared with 24 hours after operation,W/D,the concentrations of IL-6 and IL-17,mRNA ex-pression and protein content of RORγt and HIF-1α,and lung injury score were significantly decreased(P＜0.05),the concentrations of IL-10,and TGF-β,Foxp3 mRNA expression and protein content,and AQP1 protein content were significantly increased in group SF 48 hours after operation(P＜0.05).Compared with group SH,W/D,the concentrations of IL-6,IL-17,IL-10,and TGF-β,mRNA expression and protein content of RORγt,Foxp 3,and HIF-1α,and lung injury score were significantly increased(P＜0.05),AQP1 and AQP5 protein contents were significantly decreased in groups HS and SF 24 and 48 hours after operation(P＜0.05),and alveolar structure was damaged under light microscope and alveolar interstitium was filled with a large amount of edematous fluid,during which a large number of inflammatory cells infiltra-ted.Compared with group HS,W/D,the concentrations of IL-6 and IL-17,mRNA expression and protein content of RORγt and HIF-1α,and lung injury score were significantly decreased(P＜0.05),the concen-trations of IL-10 and TGF-β,Foxp3 mRNA expression and protein content,AQP1 and AQP5 protein con-tents were significantly increased in group SF 24 and 48 hours after surgery(P＜0.05),and the alveolar structure was improved under light microscope,and edema was reduced,and the number of inflammatory cells was reduced.Conclusion Shenfu injection can regulate the balance between pro-inflammatory factors IL-6 and IL-17,and anti-inflammatory factors IL-10 and TGF-β,increase the protein content of AQP1 and AQP5 in lung tissue,and decrease the W/D and injury score in lung tissue,thus alleviating lung injury in HS rats.The mechanism may be related to the regulation of HIF-1α-RORγt/Foxp3 balance.

Objective: To investigate the expectations and perceptions of nurses and patients in the intensive care unit (ICU) on the quality of nursing services, and to compare and analyze the differences and similarities between the two groups. Methods: 97 nurses and 184 patients from three ICU of three hospitals in our city were selected by convenient sampling, and their nursing service quality expectation and perception questionnaire were used to investigate. Results: The expectation scores of ICU nurses and patients on the quality of nursing service were (4.31 ± 0.63) and (4.22 ± 0.62) respectively. The scores of perception were (4.05 ± 0.58) and (3.96 ± 0.70) respectively. The difference of perception expectation score between nurses and patients is negative. The score difference between nurses in Item 3, 9~12 and responsiveness was significantly higher than that of the patients. The score difference between the entry 4~7 and the reliability dimension was significantly lower than that of the patients, and the difference was statistically significant (P<0.05). Conclusions The quality of nursing service perceived by ICU patients and nurses is relatively poor, and there are differences in reliability and responsiveness between the two patients.