Objective:Colorectal cancer is one of the common gastrointestinal tumors.Recent studies have shown that, the expression of PDEF can promote the differentiation of Secretory progenitor cells to goblet cells in the intestinal tissue.Therefore the oc-currence of colorectal cancer may be related to expression of PDEF.In this study,we tried to investigate the effects of proliferation and invasion after interference targeting prostate-derived ETS factor in colorectal cell lines HT29.Methods: HT29 cells were transiently transfected with PDEF shRNA plasmids and blank control plasmid via cathodolyte liposome transfection method.By fluorescence microscopy,RT-PCR,Western blot technique to detect the expression of PDEF mRNA and protein in normal control group,blank control group,shRNA group.The proliferation and invasion ability of HT29 cells after transfection were assessed by MTT assay and Transwell invasion assay respectively.Results: Green fluorescent protein was observed in blank control plasmid group and shRNA plasmid group.Western blot showed the reduced PDEF protein expression compared with normal control group and blank control group.Interference PDEF gene expression can significantly promote the proliferation of HT29 cells (P<0.05).The ability of cell invasion in interference group was significantly higher than the normal control group and blank control group after 48h ( P<0.05).Conclusion:Interference PDEF in HT29 cells can promote cell proliferation and invasion.

ObjectiveTo discuss application effects and student role of the self-service teaching model in biochemistry theory teaching.MethodsThe clinical students of Grade 2008 were divided into the experimental group ( 190 person ) and the control group ( 181 person ) at random.The traditional teaching model was used in the control group and self-service teaching model was adopted in the experimental group.The teaching effect was compared by the questionnaire and terminal examinations.Results The experimental group was significantly higher than the control group in average scores.Students were satisfied with the teaching effect.ConclusionThe self-service teaching method is superior compared with the traditional teaching,and the self-service teaching model can improve the teaching quality of biochemistry.