Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Adult ; Blood Cell Count ; Control Groups ; Female ; Humans ; Male ; Physicians ; Radiography, Interventional

Objective To investigate the degree of cognitive impairment and its clinical significance of Binswanger's disease(BD),leukoaraiosis(LA) and LA with cerebral infarction(LA+CI).Methods Mini-mental state examination(MMSE) and clinical memory scale (CMS) were used to evaluate the cognitive impairment in 33 patients with BD, 27 patients with LA, 31 patients with LA+CI and 30 healthy controls.Results (1)The scores of MMSE and CMS in BD group, LA group, LA+CI group were significantly lower than those in healthy control group( P

Objective To study the factors which affect the functional restoration of the elbow for adults with supracondylar or intercondylar fractures of the humerus treated with open reduction and internal fixation. Methods 22 patients with supracondylar or intercondylar fractures of the humerus were treated with open reduction and internal fixation from January, 1999 to December, 2004. They were followed up for an average of 30 months and complete clinical data were kept. The function of the elbow was evaluated with the Mayo elbow performance index. The relationship between the results and the factors —initial time of postoperative rehabilitation, fracture classification, operative approach and interval from injury to operation —were analyzed with cumulative logistic regression model of SAS software V8.2 (SAS institute, Cary, North Carolina). Results Of the 22 cases, 7 were excellent, 9 good, 5 fair and 1 poor. The excellent and good rates were 72.7%. And the odds ratio estimates of the 4 factors were 0.786, 0.299(B/A)0.221(C/A), 1.038, 0.197 respectively. Conclusions The initial time of postoperative rehabilitation, the fracture classification and the interval from injury to operation affect the functional restoration whereas the operative approach does not. Early operation, anatomical reduction, stable fixation and early rehabilitation will help improve the functional restoration.

Objective:To investigate the composition and clinical features of inpatients with congenital heart disease (CHD) complicated with pulmonary arterial hypertension (PAH) from a cardiovascular department.Methods: We retrospectively analyzed the clinical data of 238 PAH patients who were identified from 3 674 CHD inpatients in Changhai Hospital during Jan, 2001 to Jul, 2007.Results: Among the 3 674 CHD inpatients who were treated in our hospital in recent 6 years, 238 (6.48%) had PAH.PAH was found in 10.8% of patients with atrial septal defect (ASD), 2.81% of patients with ventricular septal defects (VSD), 5.57% of patients with patent ductus arteriosus (PDA), and 19.6% of patients with complex types.The mean age of patients was (45.6?17.7) years, with 49.6% of them being 40-60 years.Female patients accounted for 69.3% of the total.The composition of PAH patients was: ASD (62.6%), VSD(19.7%), and PDA(13.4%).The patients with VSD,PDA or other complex types were younger than those with ASD(P

Objective To discuss the importance of early di agnosis and treatment of primary hy-perparathyroidism in multiple bone resorptions.Methods From April 1997to April 2001,5patie nts were regarded as having multiple bone resorptions.There were 4females and 1male.The age ranged from 25to 46years(average 37years).The mean interval between symptom o nset and final diagnosis was 19.5mon ths(from8to 36months).All patients were suffering from se vere bone pain in need of strong antalgic,and the symptoms including fatigue,depression,loss of appetite and malaise,e tc.Abnormality on bone scan indicated multiple areas of skeletal involvem ent,bone radiographs showed multip le bone resorptions in skull,rib,pelvis and limbs.The range of serum calcium level was 2.27-3.22mmol /L and that o f PTH was 168-214ng /ml.Palpable neck mass was found i n three patients.Results All patients had undergone resectio n of the abnormal parathyroid.Three were parathyroid adenomas and two defined a s hyperplastic glands.All of them had pain relief after operation.The average follow-up was 15months(3-27months);the bone lesions have been repaired partially or totally s hown in X -ray examination during follow-up.Conclusion Clinically,in younger patients with multiple bone resorptions,hyperparathyroidism should be highly suspected,so that timely parathyroid surgery can be ca rried out for the treatment.[

Objective: To study the effect of different ratios of n-6 to n-3 polyunsaturated fatty acid on serum lipid in mice, on the premise that the ratio of SFA to MUFA to PUFA is 1∶1.7∶1.2 or 1∶1∶1. Methods: Ten experimental diets were made from six oils different in fatty acid composition to raise the mice for 10 w. Then the blood lipids were determined in mice fed with different experimental diets. Results and conclusion: Based on the ratio of S/M/P 1∶1.7∶1.2, which is the current dietary fatty acid intake, n-6/n-3 at 1~5∶1 will have lower serum lipid level. Based on the ratio of S/M/P 1∶1∶1 , n-6/n-3 at 1∶1 will have lower serum lipid level. Based on the ratio of n-6 /n-3 10∶1 which is the current dietary fatty acid composition abroad , S/M/P 1∶1.7∶1.2 will have lower serum lipid level.

OBJECTIVE:To detect and analyze the drug resistance of the AmpC and the extended-spectrum ?-lactamases(ESBLs)in Escherichia coli(E coli)from urinary infections.METHODS:ESBLs was detected by Phenotypic Confirmatory Test and AmpC was detected by improved three dimensional test.RESULTS:The isolating rates of ESBLs(45 strains),AmpC(8 strains)and AmpC+ESBLs in E.coli(5 strains)were 34.6%,6.2% and 3.8%,respectively.The drug resistance rates of ESBLs and AmpC-producing E.coli was higher than those of the strains without producing ESBLs or AmpC.CONCLUSION:Production of AmpC and ESBLs in ?-lactam antibiotics was the main resistance mechanism against E.coli.Carbopenems should be regarded as the first choice in clinical empirical medication for enzyme-producing strains.

Object To study the chemical constituents from leaves of Diplopanax stachyathus Hand Mazz aiming at searching for bioactive natural products Methods The compounds Ⅰ-Ⅹ were isolated by a combination of Sephedex LH 20 and silica gel column chromatographies, and their structures were identified by spectral methods Results Ten known compounds were isolated from leaves of D stachyathus Their structures were identified by means of spectroscopic analysis as: ? sitosterol (Ⅰ), betulinic acid (Ⅱ), oleanolic acid (Ⅲ), ursolic acid (Ⅳ), daucossterol (Ⅴ), quercetin 3 O ? D glucopyranoside (Ⅵ), 3,3′,4′ tri O methyellagic acid (Ⅶ), 3, 3′ di O methylellagic acid 4 O ? D xylopyranoside (Ⅷ), 3′ O methylellagic acid 4 O ? D xylopyranoside (Ⅸ), and 3, 3′,4′ tri O methylellagic acid O ? D glucopyranoside (Ⅹ) Conclusion Compounds Ⅰ-Ⅵ and Ⅸ are isolated from this plant for the first time