Objective To investigate the relationship between hOGG1 and XPD gene polymorphism and genetic susceptibility of gastric cancer.Methods This study was carried out in Dongying Shengli Petroleum Administration Bureau Hospital.Under the narrow band imaging(NBI) mode,the blood samples of a total of 98 gastric cancers and 80 controls without cancers were collected.Genetic polymorphisms of DNA repair genes were identified by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP),then its relationship with cancers was analyzed.Results Carrying 326Cys allele with the wine increased the risk of gastric cancer; Lys 751Gln genotype increased the susceptibility of the gastric cancers (OR =1.486,0.73 ～ 3.025).Conclusion Lys751 Gln genotype increases the risk of gastric cancer.

Objective To investigate the feasibility of application of plasma anticoagulant heparin and hepa-rin lithium instead of serum in clinical biochemistry.Methods The clinical data 50 healthy persons in our hospital were statistically analyzed.Results The Potassium (K),sodium (Na),calcium (Ca),low -density lipoprotein (LDL),lactate dehydrogenase(LDH),cytokines(CK),blood urea nitrogen(BUN),serum uric acid(UA),alkaline phosphatase(ALP),magnesium(Mg),the standard value of total cholesterol(CHO),serum total bilirubin(TBil), direct bilirubin(DBil),triglyceride(TG)and other items of anticoagulant heparin plasma were (4.42 ±0.27)mmol/L, (137.00 ±1.70)mmol/L,(2.56 ±0.41)mmol/L,(115.53 ±88.93)mmol/L,(5.06 ±1.18)mmol/L,(281.56 ± 67.36)mmol/L,(84.41 ±74.02)μmol/L,(0.99 ±0.18)U /L,(4.62 ±1.26)μg/L,(17.18 ±15.66)mmol/L, (2.22 ±2.66)μmol/L,(2.28 ±7.70)mmol/L,which were significantly lower than those in serum(t =-3.415,-2.017,-4.739,-3.571,-3.155,-2.778,-4.117,-7.65,-10.799,-5.677,-3.192,-8.625,-5.401,-3.483,all P <0.05.);The K,Na,TCa,BUN,UA,ALP,DBil,TP,Mg,CK,HDL -C,LDL,LDH and other items of lithium heparin anticoagulant plasma were (4.51 ±0.30)mmol/L,(137.06 ±1.56)mmol/L,(2.60 ±0.08)mmol/L, (5.08 ±1.22)mmol/L,(281.86 ±67.78)μmol/L,(84.83 ±74.82)U /L,(2.22 ±2.56)μmol/L,(74.15 ± 10.94)mmol/L,(0.99 ±0.17)μg/L,(118.47 ±92.77)U /L,(1.68 ±0.72)mmol/L,(2.55 ±0.66)mmol/L, (129.82 ±30.34)U /L,which were significantly lower than those in serum(t =-3.517,-3.273,-4.128,-3.101,4.749,-2.271,-4.586,-6.706,-10.095,-5.837,2.234,-7.309,-7.175,-14.875,all P <0.05.);The HDL -C,LDL,BUN,Cr,TBil,DBil,Na,Cl,UA,GGT,ALP,ALT,AST,TP,ALB,TBA,Mg,CHO,LDH, CK,CK -MB,AMS,TG,Glu 24 and other items of heparin lithium heparin anticoagulant plasma and serum had good correlation(r >0.800).Conclusion Plasma anticoagulant heparin and heparin lithium instead of serum is feasible in clinical biochemistry.

Adult ; Blood Cell Count ; Control Groups ; Female ; Humans ; Male ; Physicians ; Radiography, Interventional

Objective To study the nursing methods of gastrointestinal cancer patients undergoing continuous hyperthermic peritoneal peffusion chemotherapy (CHPPC) in the peri-operative period.Methods A total of 126 patients with gastrointestinal cancer underwent continuous hyperthermic peritoneal perfusion chemotherapy,and nursing care was implemented continuously,comprehensively and individually.Results All of the patients underwent continuous hyperthermic peritoneal perfusion chemotherapy successfully,without any complication occurred.Conclusions Intensive and effective nursing care is helpful to carry out continuous hyperthermic peritoneal perfusion chemotherapy safely,and avoid the complications.

Aggressive T cell non-Hodgkin lymphomas are a group of highly malignant tumors characterized by rapid progress and short survival.This is especially true for patients who have a poor response to treatment because of their drug resistance.Hence,to clarify the mechanism of this resistance will have important guiding significance in reversing drug resistance and improving poor prognosis of aggressive T cell nonHodgkin's lymphomas.

Objective To probe into the methods of postoperative nursing care for the patient undergoing domino liver transplantation.Methods The key of postoperative care included:monitoring of vital signs,the condition of cruor and the state of drainage rigorously; keeping the balance of intake and output; taking the immunosuppressive drugs correctly; proper diet; prevention of complication;giving psychological nursing.Results The patients passed the perioperative period successfully and were all cured.Conclusions To aim at the characteristic of this operation,implement holistic nursing care,is an important link to assure the patients of domino liver transplantation to recover smoothly after the operation.

BACKGROUND:There are few reports about the effect of serum-free culture on the differentiation of rat adipose-derived stem cel s into vascular endothelial cel s.
OBJECTIVE:To investigate the isolation, serum-free culture of rat adipose-derived stem cel s differentiating into vascular endothelial cel s.
METHODS:The rat adipose-derived stem cel s were isolated from male Sprague-Dawley rats and expanded to the third passage by enzymatic digestion-adherent explants method. In the experimental group, rat adipose-derived stem cel s were cultured in serum-free medium for 24 hours. In the control group, rat adipose-derived stem cel s were cultured in low-glucose Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum. After that, the cel s were cultured in inducing medium for 3 weeks to differentiate into vascular endothelial cel s.
RESULTS AND CONCLUSION:The rat adipose-derived stem cel s grew as polygonal or fusiform-shaped adherent cel s when cultured in vitro, which could stably proliferate and passage. The rat adipose-derived stem cel s showed very low expression of CD31, a cel surface marker, after passages. After directional differentiations into vascular endothelial cel s, the cel s were pebble-shaped under the inverted microscope. Expression of CD31 was up-regulated, which was much higher in the experimental group than the control group. The induced cel s in the experimental group had stronger abilities than those in the control group to swal ow Dil-labeled acetylated low-density lipoprotein and form tube-like structures on the matrigel after differentiation into vascular endothelial cel s. So, rat adipose-derived stem cel s could be highly successful y induced to differentiate into vascular endothelial cel s in vitro after serum-free culture.

This paper reviews the development of physician professionalism and its research at home and abroad in recent decades.Content,evaluation,the problems and challenges,and the affecting factors of physician professionalism are analyzed.With regard to the status quo of physician professionalism in China,some ideas to improve and build physician professionalism in China are proposed.

Objective To assess the impact of parents' occupational class and intergenerational occupational mobility on children's mental health so as to provide evidence for improving health equity.Methods Chinese general social survey 2005 (CGSS2005) dataset was used for multivariate analysis of variance (MANOVA).Results The proportion of children whose father or mother was of lowerclass occupation was 59.0％ and 62.9％,respectively.When grew up,this figure dropped to 49.8％.Those whose father was in the higher-class occupational group reported better mental health status (F =1.449,P=0.015).Downward occupational class mobility experience also increased the risk of mental illness (F =3.050,P =0.048).Conclusion This study suggests an independent relationship between mental health and parents' occupational class and intergenerational occupational mobility.

This research applied the theory of customer satisfaction model in the evaluation of the clinical information system. By means of qualitative and quantitative methods, it made validity and reliability measurement on the measurement tools in use. In addition, it extracted the structure validity of the factor regulating scale via a factor analysis, and developed the scale for clinical information system measurement These efforts provided the hospitals with the useful measurement tool to improve their information system, promoting positive and practical hospital informationziation.