Objective To explore the effect of cyclindependent kinase(CDK)2 on the replication of herpes simplex virus(HSV).Methods Dominant-negative CDK2 expressing cell line HT29Tet-On dn-CDl(2 was used as host cells to determine the role of CDK2 in HSV rephcation.HT29Tet-0n dn-CDK2 cells were infected with HSV.The activity of CDK2 Was determined by the immunoprecipitate and autoradiograph before and after HSV infection.The growth curve of HSV Was conducted.Results The CDK2 activity was induced after infection for 6 h and reached the hishest level at 9 h.HSV replicated rapidly and productively after the CDK2 was induced.Coatiusion CDK2 is required for the replication of HSV,and HSV replicates in host cells by inducing the CDK2 activity.

Cyclin-dependent protein kinase (CDK) plays an important role in the replication of herpes simplex virus (HSV) and other important human disease viruses. But which kinds of CDK are required in the replication of HSV is still not clear. In this study, we infected dominant negative CDK2 cell line with different multiplicity of infection (MOI) of HSV-1-KOS strain (abbreviated as HSV below), and the results showed that the yield of HSV depended on the MOI; the replication of HSV delayed about 3 h as compared with that of the control in the one-step growth curve replication experiments; the CDK2 activity was induced 6 h post HSV infection and reached the highest 9 h post infection; the HSV went into rapid productive replication after the CDK2 was induced. We propose herein that the CDK2 is required in the initiation of replication of HSV.
Animals ; Cell Proliferation ; Cercopithecus aethiops ; Cyclin-Dependent Kinase 2 ; physiology ; HT29 Cells ; Humans ; Simplexvirus ; physiology ; Vero Cells ; Virus Replication