ObjectiveTo investigate the effects of recombinant human erythropoietin (rhEPO) on the apoptosis of human mesenchymal stem cells induced by H2O2 and related cell signaling pathways.Methods After being cultured in vitro, human MSCs were treated with rhEPO.Phosphorylation of ERK1/2,p38 MAPK and PI3K/Akt was detected by using Western blotting.One h after pretreatment with1U/ml rhEPO,cells were cultured in the presence of1mmol/L H2O2 for1h.Cell morphology was observed under the inverted microscopy.Cell apoptosis was determined by using flow cytometry,migration assay was performed in Transwell chambers,and adhesion assay was performed by plastic dishes.ResultsRhEPO could increase phosphorylation of PI3K/Akt pathway in human MSCs,but reduce phosphorylation of p38MAPK.RhEPO had no obvious effect on ERK1/2 pathway and total proteins of Akt and p38MAPK.RhEPO could decrease apoptosis of human MSCs induced by H2O2 (P＜0.01) and the inhibitory effect was abrogated by Ly294002 but not anisomysin.Conclusion RhEPO could protect MSCs from apoptosis. Activation of PI3K/Akt pathway was involved in the effect of rhEPO on apoptosis.

Objective To determine the genotypes of human papillomavirus (HPV) and to estimate their recurrence of condyloma acuminatum (CA) among female in Xi'an.Methods HPV genotype was detected with human papilloma virus genotyping kit in 63 female patients with CA.Follow-up was carried out to observe the recurrence of CA in these patients after CO2 laser treatment.Results Of the 63 specimens,61 (96.83％) were positive for HPV,and a total of 16 HPV subtypes were identified.The predominant genotypes were HPV6 (59.2％),11 (22.95％),42 (8.20％),43 (8.20％),52 (8.20％),16 (6.56％) and 18 (4.92％) respectively.43 cases of single HPV infection was accounted for 70.49％ (43/61),mixed infection in 14 cases was accounted 22.95 ％ (14/61).61 cases of patients with laser treatment,observing the recurrence of cuses after 3-month later,it was found that the recurrence rate in higher risk group and mixed infection group was higher than that in low-risk group.Conclusion HPV6,11 is main HPV infection type of the female patients in Xi'an,the recurrence of CA is higher in patients with high risk HPV than in those with low risk HPV and mixted infection.Mixted infection of HPV and high risk HPV is a risk factor for recurrence of female genital warts in Xi'an region.

OBJECTIVE To investigate the prevalence of metallo-?-lactamase in carbapenem-resistant clinical Pseudomonas aeruginosa isolates.METHODS Sixteen strains of P.aeruginosa isolated from Tianjin General Hospital during 2005-2006 which were resistant to all tested antibiotics besides carbapenem were studied.The general PCR was adopted to amplify the resistance genes of matallo-?-lactamae.Restriction endonucleases performed restriction analysis were used to certify the type of genes of matallo-?-lactamase by different endonuclease recognition site.RESULTS Two of the 16 clinical strains of P.aeruginosa(named P02 and P16) were confirmed bearing IMP-1 metallo-?-lactamase gene detected by PCR amplification and restriction analysis.CONCLUSIONS P.aeruginosa producing IMP-1 metallo-?-lactamase is the major type in our hospital.The hydrolyzing effect of the metallo-?-lactamase is not the main machanism in these carbapenem-resistant P.aeruginosa isolates.

Dysarthria ; etiology ; Encephalitis, Viral ; complications ; Facial Paralysis ; etiology ; Humans ; Syndrome

Objective To analyze clinical characteristics of patients with gastrointestinal bleeding (GIB)and the death-related risk factors.Methods A retrospective analysis Was conducted in 414 patients hospitalized for GIB during a 16-year period of 1994 to 2009.Logistic regression analysis identified predictors of mortality.Results The mean age of the 414 patients is 83.5 years old,ranging from 65 to 96years old.The main causes of GIB were peptic ulcer(33.1%,137/414),gastroduodenal mucosal erosion (28.5%,118/414)and tumor(21.0%,87/414).The main symptom was melena(71.0%,294/414).Drugs that induced GIB were mainly non-steroidal anti-inflammatory drugs,including aspirin(11.1%,46/414),acetaminophen(8.9%,37/414)and indomethacin(1.9%,8/414).14%of patients(58/414) died of GIB in 30 days.The proportion of drug-induced GIB and gastroduodenal mucosal erosion caused GIB had increased significantly during the period of 2004 to 2009(P<0.05).Analysis of 30-day mortality risk showed advanced age,low diastolic blood pressure,high heart rate,low hemoglobin levels at presentation and hemorrhage volume in dead GIB elderly patients were significantly different compared with GIB elderly patients alive.Presence of severe comorbidity(heart failure and renal failure)and caused by cirrhosis and portal hypertension in GIB elderly patients were the only independent predictors of 30-day mortality (P<0.001).Conclusion Death of GIB patients occurred predominantly in elderly patients with severe comorbidities and systemic conditions at presentation.

Objective To follow up long-term clinical events and compare the long-term prognosis between partial and complete revascularization in octogenarians with coronary artery disease.Methods A total of 108 patients aged 80-88 years who underwent elective percutaneous coronary intervention (PCI) in cardiology department of Huashan Hospital were divided into complete revascularization group (n=47) and partial revascularization group (n=61).They were followed up for long-term major adverse cardiovascular events from Jan.1 2005 to Dec.31 2008.Results There were no statistically significant differences in age,percent of female,prevalence of hypertension,diabetes,hyperlipidemia,unstable angina,smoking and renal dysfunction between the groups(all P＞0.05).And the angiography results showed no difference at the number and location of coronary artery lesions,including prevalence of single-vessel disease [15 cases(24.6％)vs.8 cases (17.0％)],dual-vessel disease [18 cases(29.5％) vs.15 cases(31.9％)] and that of tri-vessel disease [28 cases(45.9 ％) vs.24 cases (51.1 ％)] between partial and complete revascularization groups (all P＞0.05).The follow-up time in two groups were (1235±508)d and (1216±560)d (t=0.24,P＞0.05).All-cause death [ 10 cases ( 16.4 ％) vs.8 cases ( 17.0％)],cardiovascular death [8 cases (13.1％) vs.7 cases(14.9％)],hospitalization with angina [12 cases (19.7％) vs.9 cases (19.1 ％)],with hypertension [7 cases( 11.5 ％)vs.5 cases (10.6 ％)],with cardiac arrhythmia [5cases(8.2％) vs.4 cases(8.5％)],with chronic heart failure [4 cases(6.6％)vs.3 cases(6.4％)],and with all-cause cardiovascular disease [33 cases (54.1％) vs.25 cases(53.2％)] had no significant differences between partial and complete revascularization groups (all P ＞ 0.05).Conclusions Long-term prognosis of partial and complete revascularization is similar in octogenarians with coronary artery disease.

ObjectiveTo assess the factors influencing plasma B-type natriuretic peptide(BNP) levelsin patients with chronic atrial fibrillation (AF).MethodsClinical and echocardiographic information weredetected or collected.Level of plasma BNP were measured through immunofluorescence quantitified method in222 CAF patients.The patients were divided into two groups according to left ventricular ejection fraction(LVEF) :LVEF ≥50％ (normal group) and LVEF ＜ 50％ (case groups) .LVEF was detected by transthoracicechocardiography.Fasting Blood Glucose, total cholesterol, triglycerides, highdensity lipoprotein cholesterol, low density lipoprotein cholesterol, creatinine, blood urea nitrogen, uric acid, were measured, and history ofhypertension, diabetes mellitus and smoking, were recorded.ResultsSerum levels of BNP in patients of chronicAF with LVEF ＜ 50％ were significantly higher than those with LVEF≥50％ (Mean Rank, 158.00 and 87.78 ,Z=-7.705, P ＜ 0.001) .In multiple logistic regression analysis, male gender (OR: 13.115,95％ CI 3.382 to50.860,P ＜0.001),history of hypertension(OR:3.710,95％ CI 1.104 to 12.472 ,P = 0.034) ,lower TC(OR: 0.397,95％ CI 0.214 to 0.735, P = 0.003) , LVEF(OR :0.791,95 ％ CI 0.723 to 0.867, P ＜ 0.001) , history ofsmoking(OR: 4.660,95％ CI 1.256 to 17.281, P = 0.021)were significant and independent determinants ofBNP elevation.ConclusionLower LVEF, male gender, history of hypertension and smoking, lower plasma TCare independent predictors of plasma BNP levels in patients with chronic AF.

Objective:To detect the levels of TGF-β1 in hypertensive renal function impairment patients and its clinical value. Methods: Primary hypertension patients with no target organs damage were enrolled in control group, primary hypertension patients with renal function stageⅠand stageⅡwere enrolled in renal function stageⅠgroup and stageⅡgroup. Serum TGF-β1 levels and renal function indices of three groups were compared. Results:There were no significant difference of serum creatinine and blood urea nitrogen of three groups(F=23.523, P<0.05);endogenous creatinine clearance rate of renal functionⅠandⅡgroups were lower than that of control group, TGF-β1 levels of renal functionⅠandⅡ groups were higher than of control group; TGF-β1 levels were negatively correlated with creatinine clearance rate. Conclusion:TGF-β1 level is an ideal index to reflect renal function for its level significantly changes in renal damage stageⅠ,Ⅱand has good correlation with Cin.

Objective To investigate the application value of plasma D-dimer and coagulation indicators in the patients with o-varian cancer by detecting the routine coagulation indicators and plasma D-dimer level.Methods The plasma D-dimer and coagula-tion indicators levels were measured in 84 patients with ovarian cancer and 25 patients with benign ovarian tumor.The application value of preoperative plasma D-dimer level in monitoring the disease course of ovarian cancer was evaluated.Results The plasma levels of D-dimer and FBG in the ovarian cancer group were significantly higher than those in the ovarian benign tumor control group(P <0.01);moreover the plasma levels of D-dimer and FBG in the patients with the stage Ⅲ~Ⅳ ovarian cancer were signifi-cantly higher than those in the patients with the stageⅠ~Ⅱ ovarian cancer,the differences had statistical significance(P <0.05), which prompting that the coagulation and fibrinolysis dysfunction in the patients with ovarian cancer exhibited the aggravating trend with the disease condition progression.Conclusion D-dimer may reflect the excessive activation of fibrinolysis function in ovarian tumor,which prompting the survival period and prognosis in the patients with advanced ovarian cancer.The plasma D-dimer level has the important significance to early prevention,early diagnosis,early treatment and improve the prognosis in the thrombosis oc-currence in ovarian cancer.