1.Proteomics study on liver of acute hepatic failure rats
Lei CAI ; Wailin HOU ; Yuan CHENG ; Mingxin PAN ; Yi GAO
Chongqing Medicine 2015;44(12):1592-1595
Objective To screen the differentially expressed proteinin the livetissue of the drug-induced acute hepatifail-ure rat.MethodTwenty-foumale SD ratwere randomly divided into two group,the experimental group (12 cases) wagiven D-galactosamine10g/L by intraperitoneal injection and the control group (12 cases) wagiven normal saline by intraperitoneal injec-tion .The total proteinin the livetissue samplewere extracted ,quantitated ,and subjected to separate by the two-dimension elec-trophoresis(2-DE) of isoelectrifocusing (IEF) and SDS-PAGE ,found outhe discrepanprotein spotby the software and per-formed the identification by MALDI-TOF-M.Result27 differential protein spotwere successfully identified ,and 15 up-regula-ted and 12 down-regulated proteinexpressionwere obtained in the experimental group compared with the control group .Conclu-sion The significandifferencein the expressionof protein,such acasein kinase I(CKⅠα) ,tyrosine protein kinase(PTK) ,pro-liferating cell nucleaantigen(PCNA) ,et.in the liveexisbetween the acute hepatifailure model ratand the normal one.
2.Implementation of fast tract surgery in patients undergoing liver resection
He HONG ; Mingxin PAN ; Yi GAO ; Limin KANG ; Kanghua WANG
Chinese Journal of Hepatobiliary Surgery 2015;21(2):134-137
Fast tract surgery (FTS) has been implemented in different fields of surgery to attenuate the surgical stress response and accelerate recovery.Liver resection is the preferred treatment for a variety of primary and secondary liver tumors.However,liver resection is associated with severe stress response and higher rate of postoperative morbidity and mortality.Fast tract surgery has been reported to accelerate recovery following liver resection.In this review,we summarize the recent progress of fast tract surgery in liver resection.
3.Expression of ROCK Ⅰ and TGF-?_1 in pulmonary arterioles of rat with chronic thromboembolism pulmonary hypertension
Mingxin CHEN ; Yimei ZHANG ; Wenjuan LIU ; Yufei GAO ; Liling WANG
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To investigate the dynamic expression of Rho kinase(ROCK I) and transforming growth factor ?1(TGF-?1) in pulmonary arterioles of rat with chronic thromboembolic pulmonary hypertension.METHODS: Sixty-four male Wister rats were randomly divided into eight groups: beginning control group,embolism for 3 d,1 week,2 weeks,4 weeks,8 weeks,12 weeks groups and end control group.The pulmonary thromboembolism(PTE) model was established by injecting thrombin into jugular vein two times in two weeks and each rat underwent peritoneal injection with tranexamic acid one time a day during experiment to prevent thrombolysis.The mean pulmonary artery pressure(mPAP),right ventricular hypertrophy index(RVHI),relative medial thickness of small pulmonary arteries(PAMT) and vessel wall area/total area(WA/TA) were measured.The levels of ROCK I mRNA and TGF-?1 protein in rat pulmonary artery were determined by in situ hybridization,immunohistochemistry and image analysis,respectively.RESULTS: mPAP,PAMT and WA/TA were higher respectively in embolism from 4 weeks group to 12 weeks group than those in beginning control group(mPAP: all P
4.Intra-pulmonary arterial antitumor necrosis factor-α antibody effect on ultra-structure of lung following cardiopulmonary bypass
Mingxin GAO ; Haitao LI ; Fan ZHANG ; Chen BAI ; Yang YU
Chinese Journal of Thoracic and Cardiovascular Surgery 2014;30(1):33-37
Objective To study the effect of intra-pulmonary arterial anti-tumor necrosis factor-α antibody(TNF-α Ab) on ultra-structure of lung after cardiopulmonary bypass (CPB).Methods Forty New Zealand rabbits were selected and randomly divided into four groups:group Ⅰ-Ⅲ underwent CPB; group Ⅳ only received open chest operation.In the group Ⅱ,rabbit TNF-α Ab(27 ng/kg) was dropped into the pulmonary artery when the aorta was clamped and CPB continued 30 minute.Pulmonary arterial perfusion was given to the group I instead.Blood TNF-α and neutrophils count from right and left atrium and oxygenation index in the four groups were determined perioperatively.Lung water content,TNF-αmessenger RNA,Bcl-2 protein,Bax protein,Bcl-2/Bax,apoptosis index and pathomorphological change were measured in the lung tissues.Results TNF-α Ab can restrain leukocyte accumulation and reduce releasing of TNF-α in the lung and improve oxygenation index.Moreover,TNF-α Ab can reduce the expression of Bax on alveolar epithelial cells and alleviate the decreasing of the expression of Bcl-2.It can also reduce the occurrence of apoptosis and attenuate pathomorphological changes in the lung tissue.Conclusion Intra-pulmonary arterial antitumor necrosis factor-α antibody markedly lessens the injury of inflammatory reaction and ultra-structure of lung after CPB via Bcl-2/Bax pathway.
5.Protective effect of transfusing autogeneic bone marrow derived mesenchymal stem cells on liver allograft in pig
Zhong WANG ; Yan WANG ; Mingxin PAN ; Fandong KONG ; Yi GAO
Medical Journal of Chinese People's Liberation Army 1983;0(02):-
0.05),while the changes in the serum levels of AST and ALT in the recipients in of treatment group were obviously lower(P
6.Study on the PKC in signal transduction pathway in hepatocyte ischemic preconditioning
Mingxin PAN ; Yi ZHANG ; Aihui LI ; Yi GAO
Chinese Journal of General Surgery 1993;0(03):-
Objective To investigate the mechanism of alternation of PKC activify in liver ischemia preconditioning(IP). Methods After establishment of rat liver IP model, PKC inhibitor and activator were utilized to analyze the phosphorylation of PKC and P44/42MAPKs and HSP expression, and cellular structure was also observed. All of the data were statistically analyzed. Results Compared with the control group without IP, the phosphorylation of PKC was significantly increased in IP treated models and PKC activated group(P
7.Application of continuous negative pressure of the vacuum sealing drainage dressing's aspiration in the open infected wound
Rongbo WU ; Mingxin WANG ; Dechang LIU ; Deqiang MENG ; Zhenchao GAO ; Min XIONG
Clinical Medicine of China 2013;(6):632-634
Objective To investigate the effects of the treatment of open injury and orthopedic postoperative wound infections with Vacuum Sealing Drainage.Methods Retrospective analysis of the clinical results of 32 patients with open injury or orthopedic postoperative wound infections in our hospital.Results Follow the indications and contraindications strictly and ensure right pressure,all the infection of wounds was controlled,and split skin graft survived.Without the package and pressurized fixation,the wounds healed better.Conclusion Vacuum Sealing Drainage can be considered one of the effective methods to treat the open injury or orthopedic postoperative wound infections and is worthy to be carried out as a routine.For patients with vascular rupture or defect,when there was still skin defects after vascular graft repair,as long as covered vascular anastomosis with the surrounding skin and soft tissue during the operation,there was still can use closed vacuum sealing drainage to cover the wound to prevent infection.
8.Rabbit artery bypass grafting model by using vascular anastomosis wheel
Longsheng DAI ; Yang YU ; Mingxin GAO ; Chunxiao WANG ; Fan ZHANG ; Chengxiong GU
Chinese Journal of Thoracic and Cardiovascular Surgery 2015;31(1):31-34
Objective To establish a close clinical and easy to operate animal artery bypass grafting model by using vascular anastomosis wheel.Methods 15 rabbits that weighted 2.5-3.5 kg were studied.Each animal underwent an end-toend anastomosis of jugular vein and carotid artery by vascular anastomosis wheel.Carotid ultrasound and flow detection were taken immediately and 2 months after surgery respectively,as well as morphology and pathology were recorded to analyze and evaluate the intimal hyperplasia of vein graft and arteriovenous anastomotic site.Results 14 rabbits were successfully established CABG model,however 1 rabbit died of respiratory inhibition caused of excessive anesthesia.Compared with normal carotid artery,the vein bridge showed significantly lower blood flow [(50.81 ± 1.33) ml/min vs.(70.59 ± 0.68) ml/min,P <0.01,higher PI(2.15 ±0.07vs.1.22 ±0.04,P <0.01)] immediately after surgery.Compared with the vein grafts immediately after surgery,the vein grafts 2 months after surgery showed significantly lower blood flow [(27.46 ± 2.15) ml/min vs.(50.81 ± 1.33) ml/min,P < 0.01].Compared with normal jugular vein,the vein grafts 2 months after surgery showed significantly higher intimal hyperplasia[(160.30 ± 1.78) μm vs.(49.06 ± 2.76) μm,P < 0.01],and higher number of elastic plates(12.36 ± 0.25vs.3.21 ± 0.15,P < 0.01).Conclusion The use of vascular anastomosis wheel to establish an artery bypass graft model can imitate the pathological changes of vein grafts after CABG,which can provide an ideal animal model for various researches on vein grafts.
9.The perioperative management of orthotopic liver transplantation in Chinese inbred miniature porcine
Mingxin PAN ; Fandong KONG ; Aihui LI ; Jinmin XIE ; Yi ZHANG ; Ling WANG ; Yi GAO
Chinese Journal of General Surgery 2001;0(08):-
Objective To summarise the perioperative features of orthotopic liver transplantations(OLTs) in miniature porcine,to establish mature、stable OLT models.Methods In order to observe hemodynamics and blood changes and improved perioperative management and summarise the perioperative characteristics,8 preliminary experiments(group A) and 18 experiments(group B) of OLTs were performed without veno-venous bypass(VVB) in Chinese inbred miniature porcine.Results The mean operation time was(179.6?14.3)min in our 18 OLTs,and the mean anhepatic phase time was(27.3?3.4)min.Dramatic hemodynamic and metabolic changes were identified during anhepatic phase.The oneweek survival rate was 88.9% in group B,but all the 8 pigs in group A died.The experiment group pigs urinated after 3~5hours and had normal diet after 3days.Conclusions Decreasing the operation time and stability of hemodynamics in anhepatic phase are the keys for success in OLTs without VVB.
10.Evaluate the security of the treatment of a new bioartificial liver system with experimental pig model
Longhui XIONG ; Guolin HE ; Zhi ZHANG ; Yan WANG ; Mingxin PAN ; Yi GAO
Chongqing Medicine 2014;(9):1038-1040
Objective To evaluate the security of big animals with a new bioartificial liver system .Methods Six Tibet pigs re-spectively received treatment of hybrid artificial liver and simple bioartificial liver ,observed and recorded the vital signs ,venous pressure ,transmembrane pressure and slurry pot pressure each hour ,and collected blood to make endotoxin and bacterial culture test in the zero hour ,fourth and eighth hour .Results Compared with the zero hour ,venous pressure ,transmembrane pressure ,slur-ry pot pressure were much higher in the fifth hour (P< 0 .05) ,and there were no significant difference in the rest of other time points(P>0 .05) .The mean arterial pressure and respiratory rate in all time point showed no significant changes (P>0 .05) .Com-pared with the zero hour ,the heart rate was much lower in the second hour (P<0 .01) .The values of blood endotoxin were less than 0 .5 EU/mL in the zero hour ,fourth and eighth hour from beginning ,and the bacterial culture test showed no growth of bacteria . Conclusion The experiment of big animals with a new bioartificial liver system was safe ,the efficacy in the treatment of hepatic failure could be assessed further .