OBJECTIVE:To study the pharmacokinetics and relative bioavailability of metformin hydrochloride enteric-coated tablets and validate the bioequivalence between the domestic and the imported metformin hydrochloride enteric-coated tablets.METHODS:An oral single dose(1 000 mg)of domestic or imported metformin hydrochloride enteric-coated tablets was given to 26 healthy volunteers in a randomized crossover study.The time-concentrations of metformin hydrochloride were determined by HPLC.The pharmacokinetic parameters as well as relative bioavailability of the domestic tablets were computed.RESULTS:The concentration-time curves of the two kinds of metformin hydrochloride enteric-coated tablets were in line with one-compartment model.The main pharmacokinetic parameters of the domestic vs.the imported metformin hydrochloride enteric-coated tablets were as follows:Cmax(84.9?16.4)?g?L-1 vs.(84.5?13.8)?g?L-1;tmax(0.67?0.07)h vs.(0.77?0.08)h;t1/2 Ke(1.90?0.17)h vs.(1.79?0.19)h;AUC0～t(161.9?16.8)?g?h?L-1 vs.(157.2?19.7)?g?h?L-1;AUC0～∞(179.7?18.2)?g?h?L-1 vs.(173.4?19.2)?g?h?L-1.The relative bioavailability of the domestic enteric-coated tablets was(109.0?7.2)% as against the imported enteric-coated tablets.CONCLUSION:The two kinds of tablets were bioequivalent.

To analyze the clinical characteristics of postmenopausal ovarian tumors and explore the predict indicators and treatment for ovarian benign and malignant tumors.A retrospective analysis was performed for clinical data and pathological results of 125 patients with postmenopausal ovarian tumors from January 2008 to January 2013.The symptoms of malignant patients included abdominal pain (n =30),bloating (n =11) and weight loss (n =5).It had significant difference compared with benign tumors (P ＜0.05).Ultrasound showed that there was 44 cystic and solid tumors with 22 malignancies and 57 cystic tumors with 5 malignancies.There was significant difference in morbidity of malignant ovarian tumors between them (P ＜ 0.05).The incidence of malignant tumor in 5-10 cm group was 56％.There were significant differences compared with the ＜ 5 cm group (P ＜ 0.05).The specificity of ultrasound plus a combined detection of CA125 for malignant epithelial tumor of ovary was 91％ with a sensitivity of 93％.Thirty-one cases of malignant tumor were accurately diagnosed with preoperative ultrasound.Ultrasound plus CA125 could help distinguish the characteristic of tumors.

A total of 78 pelvic abscess cases were confirmed by operation retrospectively.Group A underwent celiotomy ( n =30 ) while group B laparoscopic surgery ( n =48 ).Medical history , clinical symptoms and outcomes were analyzed.Operative durations of groups A and B were ( 90 ±21 ) and ( 70 ± 14) min, the amount of hemorrhage (70 ±24) and (46 ±24) ml and wound dehiscence rate 20% and 0 respectively.There was significant inter-group difference.And the efficacy of group B was better than that of group A.The postoperative recurrence rate was 13% and 4% and there was no significant difference.Laparoscopic surgery is suitable for treating pelvic abscess.It is mini-invasive, has a quick recovery and does not increase operative complications.

To improve the quality education of medical students,we should attach importance to the construction of teaching staff,improve learning environment,strengthen selected course construction and arrange for students to take social practice.

The practical teaching of clinical medicine is in progress on the last stage of the 5-year university life,so it has the feature of the employment preparation and is influenced by a lot of factors.To make the practical teaching of clinical medicine take the right turning and get a satisfactory result,we should not only lay stress on the construction of teachers'contingents,but also bring the students'initiative to play and tighten up the management.

Objective:To investigate the relationship between some common risk factors of the cerebralvascular diseases and PD.Methods:Case-control study was carried out.Blood glucose,low density lipoprotein cholesterol(LDL-C)and homocysteic acid(Hcy) levels were detected by routine methods.The intima-media thickness(IMT)of carotid artery was measured by color Doppler ultrasound.The PD patients were graded by Unified Parkinson's Disease Rating Scale(UPDRS).Results:The blood glucose and Hcy in PD patients were higher than that in control group,and the LDL-C,IMT of carotid artery in PD were lower than that in control group.The Hcy was positively related to risk of PD,while IMT of carotid artery and LDL-C were negatively related to risk of PD by Spearman analysis.Conclusion:The Hcy level is a risk factor of PD.The LDL-C and IMT of carotid artery in PD patients were lower than that in control group,which suggests that the probability of PD patients who suffer from atherosclerosis is small.

Objective To establish a method for determination of trace phenol in waste water. Methods It is reported that trace amounts of phenol can inhibit the decoloring reaction of methyl red by potassium bromate in dilute sulfuric acid medium. Results The linear range of this method was 0-17 ?g/25 ml for phenol with detection limit of 6.53?10-9 g/ml, RSD was 0.96%-1.70%, recovery rate was 97.8%-102.0%. Conclusion The proposed method is simple, sensitive and can be applied to determine trace phenol in waste water with satisfactory results.

Objective To establish a real-time fluorescent quantitative PCR ( FQ-PCR ) method for detection of murine norovirus ( MNV) in laboratory mouse and provide the basis for establishment of a standard detection method for MNV.Methods Specific primers were designed and MNV DNA standards were prepared according to the MNV genome sequences published on NCBI .The specificity, sensitivity, repeatability and stability of the established Q-PCR method were tested.The established Q-PCR method was applied to detect 766 mouse caecum content samples to explore preliminarily the infection status of laboratory mice in Beijing .Results No cross reaction showed in human norovirus and feline calicivirus with the established Q-PCR method.The sensitivity was up to 10 copies/μL.The coefficient of variation ( CV) of intra-assay and inter-assay was less than 2%.There were 301 positive cases detected in the 766 samples of laboratory mice.Conclusions The established FQ-PCR method is accurate and effective with high specificity , sensitivity and repeatabiliy in the quantitative detetion of nucleic acid , and can be applied to rapidly and quantitatively screen MNV in laboratory mice .

Objective To evaluate the effect of sufentanil combined with dexmedetomidine on analgesia in patients with pulmonary lobectomy.Method Totally 80 patients with pulmonary lobectomy in Yanan University Affiliated Hospital were collected from January 2014 to August 2016,which randomly divided into two groups:patients in control group (n-40) were given sufentanil 0.05 μg/(kg·h),and patients in observation group (n =40) were given sufentanil 0.03 μg/(kg·h) combined with dexmedetomidine 0.07 μg/(kg·h),The differences of hemodynamic change,VAS and Ramsay score,and adverse reaction at 1 h,6 h,12 h,24 h and 48 h after operation were compared.Results The hemodynamic change of all patients were relatively stable,and the more stable group B with lower MAP,HR and DBP at 12 h,and SBP at 6 h after operation further contributed the recovery of patients,which difference compared with group A was significance (P ＜ 0.05).However,the difference of SpO2 at the same time after operation was no significance.The VAS score blow 4 showed that the analgesia of all patients was satisfying.Comparing group B with group A,the VAS score was lower after 6 h at post-operation,which difference was significance (P ＜ 0.05).The Ramsay score of group B was higher than group A at 6h and 12 h,which difference was significance (P ＜ 0.05).The Ramsay score with range from 2 to 4 showed the sedation of all patients was not deficient or excessive.The incidence of nausea and vomit (2.5％) and dizziness (0％) in group B was lower than group A,with significant difference between two groups (P ＜ 0.05).Conclusion Combination dexmedetomidine with sufentanil to relief postoperative pain in patients undergoing pulmonary lobectomy is deserved popularization in clinic,which not only increases the efficiency analgesia and sedation,improves hemodynamic,but also decreases sufentanil-induced side-effect.

Objective To study the relationship between voice acoustic analysis(VAA) and voice handicap index (VHI) in patients with vocal cord polyps,and to investigate the characteristic of the VHI.Methods 35 subjects with normal voice and 35 patients with vocal cord polyps underwent VHI,were tested by VAA.Results The parameters such as APQ、jitter、shimmer、NHR in patients with vocal cord polys were much higher than those in the normal voice group.The average score of VHI was 43.32?4.66,while the average score of the normal voice group was 12.51?1.88.There was no correlation between VHI and VAA.Conclusion Since there is no correlation between VHI and VAA in patients with vocal cord polys.One can not speculate the VHI score by the voice acoustic analysis.