Objective To study microsurgery for craniopharyngioma and factors of influencing prognosis . Methods 62 cases with craniopharyngioma through microsurgery were enrolled as research subjects .According to the tumor characteristics,they were used through pterional,subfrontal approach,endplate and a lateral frontal approach . Results The tumor resection was 40 cases,12 cases of subtotal resection ,10 cases of subtotal .38 cases of pituitary stalk were reserved,cutting 9 cases,not-identified 15 cases.Postoperative blood electrolyte imbalance was 33 cases, 15 cases of mild to moderate diabetes insipidus ,seizures 1 case,1 case of persistent fever coma .Postoperative follow-up 6 months to 72 months,57 cases was followed up,5 cases were lost.56 cases could have normal life ,study and work.Vision was restored in 53 cases,2 cases unchanged ,down 1 case.A total of 9 cases were recured during follow-up,no recurrence total resection ,subtotal resection recurrence in 2 cases,subtotal resection recurrence seven cases (to-tal resection and subtotal resection χ2 =4.28,P<0.05;subtotal gastrectomy and subtotal resection ,χ2 =6.27,P<0.05).Follow-up of 37 cases of purely suprasellar tumor recurrence in 4 cases,the saddle 20 cases,5 cases of recur-rence,there was a significant difference between the two groups (t=5.08,P<0.05).57 patients preoperatively ele-vated CRP 32 cases,6 cases of recurrence,CRP normal 25 cases,3 cases of recurrence,there was a significant differ-ence between the two groups(t=4.96,P<0.05).Conclusion Selecting the appropriate surgical approach and pro-tecting the hypothalamus and pituitary stalk during the surgery were critical .Resection,tumor location,and tumor in-flammation were prognostic factors .

Objective To determine MIF expression in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma, and the relationship between MIF and the clinical pathologic features of colorectal carcinoma.MethodsMIF expression was determined by immunohistochemical staining ELISA was used to detect the level of MIF in serum samples. Results The percentage of MIF expressing epithelial cells and MIF positive expression intensity were progressively increased in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma. There was significant difference among three groups (P

Rhesus monkey (Macaca mulatta)ABO blood typing was carried out by agglutination test with anti human sera, and monoclonal antibodies against A and B blood group antigcus Blood Samples of 113 monkeys were tested. The results showed that there wete no human like A and B agglutinins and anti-A and anti-B agglutnins on the surface of the red blood cells and in the sera of monkeys. How ever human like blood group substances were found in monkey's galiva lemonsliated by agglutination inhibition test. The ABO phenotype frequoncies of Rhesus monkey were as follows: A-17. 70 %;B-52,21 %;AW-20, 36% and O-9. 73 % which of ther from foreign reports.

Objective To investigate the effect of tripterygium glycosides on the resistance to immune rejection after allogeneic islet transplantation in mice.Methods Twenty C57BL/6 mice were treated with STZ diabetes mellitus and transplanted the islets from Balb/c mice donor,then recipient mice were randomly divided into two groups:triptolide group and model control group(n=10),and were intraperitoneal injected with tripterygium glycoside solutin and equivalent solvent of 5 mg·kg-1·d-1 for 14 days.Blood glucose and body weight were measured within 4 weeks after transplantation.Two weeks later,two groups of grafted islets were stained by HE staining and immunohistochemical staining,the expression of IL-2 protein were detected by Western blotting.Results The level of blood glucose were decreased to normal in the triptolide group and model control group after islet transplantation,but blood glucose gradually increased in the model control group after two weeks.Compared with the model control group,the inflammatory cells were less infiltrated and the immunohistochemical staining of insulin was deeper in the triptolide group.The expression of IL-2 in the triptolide group was significantly decreased(P<0.05).Conclusion Tripterygium glucoside could significantly decrease the inflammatory cell infiltration and inflammation factor expression in the allogeneic islet recipients to reduce the immune rejection and improve graft survival.

Porous surfaces have an important effect on bioactivity of titanium implants. In this study, two micro/nanostructural titanium surfaces were prepared by chemical and electrochemical method. The two samples had different diameters of nanotubes. Tests of biomineralization and codeposition in simulated body fluid and bovine serum albumin (BSA) were carried out in order to evaluate the bioactivity of micro/nanostructural titanium surfaces. The information of the surfaces was detected using scanning electron microscope and X-ray diffractometer. The results showed that the bioactivity of micro/nanostructural titanium increased with the diameter of nanotubes. Furthermore, the existence of BSA can accelerate biomineralization and decrease the crystallinity of hydroxyapatite coating.
Biocompatible Materials ; Coated Materials, Biocompatible ; chemistry ; Materials Testing ; Microscopy, Electron, Scanning ; Microtechnology ; Nanostructures ; chemistry ; Porosity ; Prostheses and Implants ; Surface Properties ; Titanium ; chemistry

OBJECTIVETo investigate the effects of nano-lead exposure on learning and memory and iron homeostasis in the brain of the offspring rats on postnatal day 21 (PND21) and postnatal day 42 (PND42).

METHODSTwenty adult pregnant female Sprague-Dawley rats were randomly divided into control group and nano-lead group. Rats in the nano-lead group were orally administrated 10 mg/kg nano-lead, while rats in the control group were administrated an equal volume of normal saline until PND21. On PND21, the offspring rats were weaned and given the same treatment as the pregnant rats until 42 days after birth. The learning and memory ability of offspring rats on PND21 and PND42 was evaluated by Morris water maze test. The hippocampus and cortex s amples of offspring rats on PND21 and PND42 were collected to determine iron and lead levels in the hippocampus and cortex by inductively coupled plasma-mass spectrometry. The distributions of iron in the hippocampus and cortex were observed by Perl's iron staining. The expression levels of ferritin, ferroportin 1 (FPN1), hephaestin (HP), and ceruloplasmin (CP) were measured by enzyme-linked immunosorbent assay.

RESULTSAfter nano-lead exposure, the iron content in the cortex of offspring rats on PND21 and PND42 in the nano-lead group was significantly higher than those in the control group (32.63 ± 6.03 µg/g vs 27.04 ± 5.82 µg/g, P<0.05; 46.20 ±10.60 µg/g vs 36.61 ± 10.2µg/g, P<0.05). The iron content in the hippocampus of offspring rats on PND42 in the nano-lead group was significantly higher than that in the control group (56.9 ± 4.37µg/g vs 37.71 ± 6.92µg/g, P<0.05). The Perl's staining showed massive iron deposition in the cortex and hippocampus in the nano-lead group. FPNl level in the cotfex of offspring rats on PND21 in the nano-lead group was significantly lower than that in the control group (3.64 ± 0.23 ng/g vs 4.99 ± 0.95 ng/g, P<0.05). FPN1 level in the hippocampus of offspring rats on PND42 in the nano-lead group was significantly lower than that in the control group (2.28 ± 0.51 ng/g vs 3.69 ± 0.69 ng/g, P<0.05). The escape latencies of offspring rats on PND21 and PND42 in the nano-lead group were longer than those in the control group (15.54 ± 2.89 s vs 9.01 ± 4.66 s; 6.16 ± 1.42 s vs 4.26 ± 1.51 s). The numbers of platform crossings of offspring rats on PND21 and PND42 in the nano- lead group were significantly lower than those in the control group (7.77 ± 2.16 times vs 11.2 ± 1.61 times, P<0.05; 8.12 ± 1.51 times vs 13.0 ± 2.21 times, P<0.05).

ONCLUSIONn Nano-lead exposure can result in iron homeostasis disorders in the hippocampus and cortex of offspring rats and affect their learning and memory ability.

Animals ; Cerebral Cortex ; drug effects ; metabolism ; Female ; Hippocampus ; drug effects ; metabolism ; Homeostasis ; Iron ; metabolism ; Lead ; toxicity ; Learning ; drug effects ; Maternal Exposure ; adverse effects ; Memory ; drug effects ; Pregnancy ; Rats ; Rats, Sprague-Dawley

Objective: To investigate the clinical effect of Astragalus injection combined with meropenem in the treatment of severe infection patients and its influence on T lymphocyte subsets and inflammatory factors in peripheral blood. Methods: From January 2016 to December 2018, 80 patients with severe infections in Yiwu Fuyuan Private Hospital were selected and divided into observation group (n=40) and control group (n=40) according to single blind random table method.The observation group was treated with Astragalus injection combined with meropenem, while the control group was treated with meropenem.Both two groups were treated for 10 days.The therapeutic effects, changes of body temperature, heart rate and respiratory rate, changes of T lymphocyte subsets and inflammatory factors in peripheral blood, and negative time of bacterial culture before and after treatment were compared between the two groups. Results: The total effective rate of the observation group (92.50%) was higher than that of the control group (70.00%) (χ²=6.646, P<0.05). After treatment, the levels of CD₃⁺ [(62.38±4.51)%], CD₄⁺ [(40.63±2.15)%] and CD₄⁺ /CD₈⁺ (1.63±0.18) in the observation group were higher than those in the control group [(57.27±3.19)%, (35.47±1.93)% and (1.30±0.21)] (t=5.850, 11.296, 7.546, all P<0.05). After treatment, the levels of CRP [(28.79±8.74)mg/L], PCT [(0.56±0.12)g/L] and TNF-α [(157.93±20.47)pg/mL] in the observation group were lower than those in the control group [(43.71±12.67)mg/L, (1.10±0.23)g/L and (247.16±34.64)pg/mL] (t=13.640, 13.165, 14.026, all P<0.05). The negative turning time of bacterial culture in the observation group [(4.36±1.29)d] was shorter than that in the control group [(7.43±1.57)d] (t=9.493, P<0.05). Conclusion Astragalus membranaceus injection combined with meropenem has significant clinical effect in the treatment of patients with severe infections.It can improve cellular immune function and reduce inflammatory reaction, which is worthy of clinical reference.

Objective To study the mechanism of differentiation of high glucose induced M2 macrophages used by high glucose medium stimulation. Methods Raw264.7 (murine macrophage cell line)was cultured and stimulated by 4. 25% glucose medium, and mannitol medium was used as osmotic pressure control. Cells were harvested at 0h,4h,8h and 12h to examine the expression of CD206 and Arg-1 and the activation of PI3K/Akt signaling pathway. After blocking the PI3K/Akt signaling pathway by LY294002(the specific inhibitor of PI3K),the expression of Arg-1 was examined by western blot. Results The expression of Arg-1 was increased from 8h while CD206 reached the peak at 12h induced by high glucose which indicated the activation of M2 in a time-dependent manner. Akt was phosphorylated at 8h stimulated by high glucose medium. The specific inhibitor of PI3K reduced the expression of Arg-1 by blocking the phosphorylation of Akt. Conclusion High glucose, rather than high osmotic pressure,induced macrophages polarized into M2 via activation of PI3K/Akt signaling pathway.