Objective To compare the treament to adhesion ileus between laparoscopic surgery and traditional surgery.Methods 59 cases of patients with adhesion ileus were selected.32 patients were given laparoscopic adhesion ileus loose solution method.And the other 32 patients were given traditional surgery.Results The operation time(t =12.135,P =0.002),peristalsis recovery time (t =9.053,P =0.001),postoperative infection rate (x2 =8.346,P =0.004),obstruction again (x2 =4.863,P =0.026) and hospital stays (t =4.724,P =0.028) in laparoscopic surgery group was significantly better.The differences between both groups were statistically significant (P ＜0.05).Conclusion Compared with the traditional open surgery,laparoscopic adhesion ileus loose solution with surgical trauma is small.Operation time and the postoperative recovery time is short,It can be used as the main treatment of adhesion ileus.

Objective To investigate the effects of hypoxia on the rheologic properties of leukocytes in microcirculation of rats, and its relationship with the expressive changes of CD18 of neutrophils. Methods Hypobaric chamber was used to simulate the plateau condition for the establishment of animal hypoxic model. Adult Wistar rats were randomized in three groups: control group, short-term hypoxia group (Hypobaric chamber simulated 5000m altitude for 3days) and long-term hypoxia group (Hypobaric chamber simulated 5000m altitude for 30 days). Intravital microscopy and computer image analysis system were used to evaluate the rheologic properties of leuckcytes in mesentery microcirculation. Neutrophils of the three experimental groups were purified and the expression of CD18 was determined by flow cytometer. Results Compared with control group, the rheologic properties of leukocytes were changed obviously at both short-term hypoxia and long-term hypoxia groups, including the number of leukocytes rolling and adherenced on endothelium increased, TLECT (total leukocyte-endothelium contact time) obviously extended (P

Objective To explore the effects of acute or chronic hypoxia on the viscoelastic properties of polymorphonuclear leukocyte (PMN) of rats. Methods Hypobaric chamber was used to simulate high altitude condition. Fifteen adult Wistar rats were randomized into 3 groups: control, acute hypoxia (5 000 m for 3 d) and chronic hypoxia (5 000 m for 30 d). Micropipette aspiration technique was used to evaluate the biomechanical properties of PMNs of rats and the experimental results were fitted with a three-element standard linear solid model. Results The viscoelastic coefficients of PMNs in acute hypoxic condition [K1=(86.66?26.84) Pa, K2=(399.89?22.03)Pa, ?=(4.23?1.82) Pa?s] or those values in chronic hypoxic condition [K1=(179.61?26.31) Pa, K2=(472.12?30.36) Pa, ?=(9.21?2.65) Pa?s] were significantly higher than the corresponding value of PMNs in control(P

The left and right cardiac function of 11 patients with chronic mountain sickness was studied before and after oxygen administration. It was found that after pure oxygen was inhaled, the internal diameter of the right ventricle, the ratio between right and left ventrcular diameters, and the heart rate were decreased significantly, while the ?-wave excursion of the pulmonary valve and the EF slope of the mitral valve were increased obviously. However there was no change of the systolic time interval of both the ventricles and of the left ventricular systolic function. The results suggest that oxygen inhalation could relieve hypoxic pulmonary hypertension and improve left ventricular diastolic function.

[Objective] To explore the method for knee joint osteoarthritis. [Method] 97 patients take the said method above, observe its effect. [Result] 41 cases were excellent, 29 good, 22 just so so, 5 bad, the total effective rate was 94.85%. [Conclusion] TCM external cleansing coordinated with small needle-knife has good clinical effect.

AIM:To determine the right ventricular angiotensin converting enzyme2 (ACE2) activity in adult SD rats under normoxia and hypoxia environment,and to detect the relationship between ACE2 and high altitude heart disease initially. METHODS:Adult male Sprague Dawley (SD) rats were raised under hypoxia environment simulated to high altitude (5 000 m,23 h/d),then divided into hypoxia 1 d,15 d and 30 d groups randomly. The control group was set up under normoxia environment. The right ventricular function,ponderal index,pulmonary artery pressure and right ventricular ACE2 activity were determined. The effect of captopril or nitrendpine on cardiac ACE2 activity in hypoxia 30 d group was also observed. RESULTS:The dramatic up-regulation of cardiac ACE2 expression of mRNA and protein and its activity in hypoxia 30 d group was observed,together with obvious increase in right ventricular function,ponderal index and pulmonary artery pressure. Although captopril or nitrendipine depressed pulmonary artery pressure and cardiac function dramatically,no significant alteration of right ventricular ACE2 activity was detected. CONCLUSION:Chronic hypoxic exposure promotes the ACE2 expression and activity in right ventricular,indicating that ACE2 may be correlated to the changes of cardiac architecture and function induced by hypoxia. Up-regulation of ACE2 expression maybe contribute to the increase in right ventricular ACE2 activity,and pulmonary artery hypertension may not be the main reason for the changes of ACE2 activity under hypoxia environment.

AIM：To investigate the roles of nitric oxide (NO) and endothelin-1(ET-1) in regulation of coronary vascular reserve (CVR) during hypoxia.METHODS： CVR were measured with 99m TC radiolabelled frog RBC, the changes of NO-2，ET-1 contents， nitric oxide synthase(NOS) activity and the myocardial morphometry were observed. RESULTS： (1) Acute hypoxia caused an increase in left and right ventricular myocardial blood flow，myocardial NO-2，ET-1 contents，NOS activity，but CVR in the left and right ventricle were decreased compared with the control group.(2) Intermittent hypobaric hypoxia for 90 days did not lead to significant change in left ventricular CVR，myocardial ET-1/NO-2 ratio. However, right ventricular myocardial ET-1 contents，ET-1/ NO-2 ratio were increased，right ventricular CVR and myocardial NO-2 contents were decreased. We also observed that perivascular collagen，arterial wall thickness in right ventricle， hematocrit，RV weight index were augmented. CONCLUSION： Rest myocardial blood flow was increased，CVR was decreased；The decreased coronary vascular reserve during chronic hypoxia might be resulted from the increased hematocrit，arterial wall thickness，perivascular collagen，ET-1 content, the decreased NO content and right ventricular hypertrophy

AIM: This study was designed to investigate the protective role of hypobaric hypoxic pretreatment (HHPT) on hippocampal neurons in Babl/c inbred mice. METHODS: HHPT was produced by simulating a 7 000 m high altitude 2.5 h/d for 3 d. At 36 h after last time decompression, three subgroups consisted of both the control and pretreatment mice were subjected to the 12 000 m high altitude hypobaric hypoxia for 4 h, the severe ischemia produced by bilateral common carotid artery occlusion for 18 min, and the severe ischemia/hypoxia produced by permanently ligation of right lateral common carotid artery followed by a 8 000 m high altitude hypobaric hypoxia for 4 h, respectively. The extents of protection to hippocampal CA1 neurons by HHPT were evaluated by accounting the number of intact neurons between HHPT and control group. RESULTS: The results indicated that HHPT protected hippocampal neurons against severe hypobaric hypoxia, severe ischemia, and ischemia combined with hypobaric hypoxia. CONCLUSION: Hypobaric hypoxic pretreatment induces a delayed protection to hippocampal neurons against hypoxic and ischemic injuries.

Objective To evaluate the effects of high intensity focused ultrasound (HIFU) on hepatic cancer cells spreading in blood.Methods AFP mRNA in peripheral blood of 19 patients with hepatocellular carcinoma was detected before and after HIFU therapy by RT PCR.Results ①Before HIFU therapy, 11 of 19 cases were AFP mRNA positive (57.9%), while the control group were all negative. AFP mRNA was correlated with some clinical parameters such as serum AFP level, tumor size, portal vein embolism and extrahepatic metastasis ( P 0.05). ③After one week of HIFU therapy, the AFP mRNA positive rate of the group with tumor size less than 8 cm was much lower than that of tumor size larger than 8 cm ( P

0.05).Conclusion TP may have protective effects on chronical hypoxia induced polycythemia in rats.