Objective To establish the normal reference ranges of T lymphocyte subsets in healthy Zhuang ethnic minority across different age/ gender groups in Guangxi for providing the scientific guidance for clinical diagnosis and treatment evaluation of immunodeficiency diseases and viral infections as well as relat-ed researches. Methods Peripheral blood samples were collected from 405(196 male and 209 female) healthy subjects of Zhuang ethnic group including 82 adolescents and 323 adults. Single platform FACSCali-bur flow cytometry analysis was performed to detect the absolute counts of T lymphocyte subsets(CD45+, CD3+,CD4+ and CD8+)in peripheral blood samples. Results Reference ranges of T lymphocyte absolute counts in healthy Zhuang Chinese aged 14 to 59 were 2 377±696,1 503±459,802±253,581±243 and 1. 52±0. 57 for CD45+,CD3+,CD4+,CD8+ and CD4+ / CD8+. Significant differences in CD45+,CD3+ and CD8+ T lymphocytes were found between adolescents and adults(t = 0. 000,P﹤0. 05),but no significant difference in CD4+ T lymphocytes was observed between the two groups of people(t = 0. 373,P﹥0. 05). Significant difference between male and female groups was observed only in the absolute counts of CD8+ T lymphocytes(t= 0. 036,P﹤0. 05). The absolute counts of T lymphocyte subsets were on the decline along with the growth of the age. Conclusion This study indicates that the counts of T lymphocyte subsets vary with ethnic group,age and gender. Therefore,it is important to establish normal reference ranges of T lym-phocyte subsets for local healthy people based on gender and age for accurate evaluation of local patients' im-mune system status and treatment efficacy.