Objective To study the effect of IL-6 and IL-8 on the MMP-9 secretion of endometrial stromal cells in women with endometriosis to explore the function of IL-6 and IL-8 in the adherence and planting process of ectopic endometrium. Methods The endometrial stromal cells were cultured in DMEM/F12 medium containing different concentrations of IL-6 or IL-8 for 24 hours, then the media were collected to measure the levels of MMP-9 by ELISA. Results The levels of MMP-9 in control stromal cells cultured with high concentration of IL-6 were significantly higher than those without IL-6 (P

Objective To investigate the role of Kit signal pathway in the proliferation and spontaneous rhythmic motility of interstitial cells(ICs)in the bladder of guinea pigs.Methods Fourteen Guinea pigs aged 45-60 days were administered with Imatinib intragastrically for 20 and 40 days.Three were fed on normal saline as control.The morph and number of ICs were observed by immunofluorescence,Kit protein expression was detected by Western blot and spontaneous rhythmic motility of bladder were investigated in in vitro organ bath.Results Quite a few ICs were observed in the bladder of controls.After administration of Imatinib,the amount of ICs and the expression of Kit protein decreased gradually,and the spontaneous rhythmic contraction weakened.Conclusion ICs might be the pacemaker of bladder in guinea pigs.Kit protein might play an important role in the survival and functional maintenance of ICs.

OBJECTIVETo evaluate the significance of cerebral protection applied at carotid endarterectomy (CEA) at perioperation, intraoperation, and postoperation.

METHODSThirty patients underwent CEA with application of cerebral protection at perioperation, intraoperation, and postoperation in our hospital from January 2002 to August 2005. Perioperative carotid Doppler ultrasound and computed tomography angiography (CTA) were applied in 29 patients and carotid angiography applied in only one patient. The key methods of intraoperative cerebral protection included general anaesthesia, selective shunting, monitoring of transcranial Doppler, and careful manipulation. The methods of postoperative cerebral protection included leaving tracheal cannula and brain ice-bag, maintaining normal blood pressure, and applying dehydrant under guidance by monitoring of transcranial Doppler.

RESULTSAmong all the 30 patients, shunts were used in 10 patients (33%), and angioplasty by patch was applied in 17 patients (57%). No cranial nerve-associated complications or death was documented.

CONCLUSIONApplication of cerebral protection at CEA at perioperation, intraoperation, and postoperation can effectively prevent the occurance of cranial nerve-associated complications.

Aged ; Anesthesia, General ; Diuretics ; administration & dosage ; Endarterectomy, Carotid ; Female ; Follow-Up Studies ; Humans ; Intubation, Intratracheal ; Male ; Middle Aged ; Monitoring, Intraoperative ; Perioperative Care ; methods ; Treatment Outcome ; Ultrasonography, Doppler, Transcranial