Objective: To compare the effects of three fluoride-containing agents on the remineralization of deciduous teeth in vitro. Methods: 36 deciduous teeth were randomly divided into four groups with 9 in each. The teeth were fenestrated on the labial middle surface and the rest parts of the teeth were covered with red nail polish.Every exposed area was etched,rinsed and puffed.Then the exposed areas were treated with 100 g/L(NH 4) 2MoO 2F 4(group ①) , 380 g/L Ag(NH 3) 2F (group ②) and APF-LaCl 3(group ③) for 3 minutes respectively, those in group④ were treated with deionized H 2O as the controls.Then,the teeth were placed into a bottle containing 5 ml remineralization solutions. 4 days later, the concentration of Ca 2+ in the remineralization solutions was measured. Results: The Ca 2+ concentrations (mmol/L) in the remineralization solution of group ①,②,③ and ④ were 0.338 9?0.116 5,0.570 0?0.145 5,0.517 8?0.084 8 and 0.120 4?0.046 8 respectively (P0.05:②vs③). Conclusion: The results indicate that all of the three fluoride-containing agents can promote the remineralization of deciduous teeth in vitro, and that both APF-LaCl 3 and 380 g/L Ag(NH 3) 2F are more effective than 100 g/L(NH 4) 2, MoO 2 F 4.

Periostin, a extracellular matrix protein structurally similar to fasciclin proteins, is expressed specifically in various tumor tissues and correlates with tumor progression and prognosis. Periostin prevents the apoptosis of tumor cells, promotes tumor growth and can induce angiogenic signaling cascades through αvβ3-FAK and Flk-1/KDR. It also contributes to epithelial-mesenchymal-transformation promoting tumor invasion and metastasis. Periostin maybe a potential therapeutic target of cancer treatment.

Objective To evaluate the statues of P-selectin and chang of D-dimer in vivo in patients with deep venous thrombosis(DVT)and to observe how to vary under interneving of medicines.Methods P-selectin and D-dimer of fourty patients and twenty normal subjects were fluo-rescencelabled with its corresponding monoclonal antibodies by flow cytometry(FCM)and immune method respectively.Results In the early stage,P-selectin and D-dimer of patients with DVT is higher than that in normals,and they significantly decreased in different time after patients were treated.Compared with patients who were used on-sodium ozagrel,patients used sodium ozagrel have different P-selectin(P＜0.05),but D-dimer was similar(P＞0.05)after fourteen days.One month later,P-selectin and D-dimer of DVT patients were lower.However,the positive rate of P-selectin of DVT was still higher than normal subjects.Conclusions The platelet has been actived in vivo in patients with DVT,so do fibrinolysis.Sodium ozagrel can decrease the action of platelet.P-selectin and D-dimmer may be used in diagnose.Post-discharge patients are still high-risk group and must be followed-up regularly.

With techniques of biochemical isolation and pruification, a semi-purified preparation of new born calf liver (BLS) with the peculiarity of low molecular weight has been found to have tumor surppressor activity inhibiting selectively the growth of murine myeloid leukemic cell (L801). But its effect is less marked against normal bone marrow granulocyte / macrophage progenitors in in vitro liquid or agar culture. Mice after inoculation of L801 cells died within 15 days due to development of leukemia. After consecutive injections of BLS, 25% - 53.8% of treated mice survived. Furthermore, it has been found that the survival rate was raised from 50% to 66% when Cyclophosphamide was first injected in the dose of 300?g / kg body weight once, followed by the consecutive injections of BLS. NO pathological changes have been observed in the mice which survived longer than 100 days after inoculation of L801 leukemic cells.

Let-7 is one of the most widely studied in miRNAs at present. let-7 can inhibit cell proliferation, promote cell differentiation and apoptosis, etc. Reduced expression of let-7 in different human tumor tissues or cells is accompanied by the changes of its target genes expression, which suggests that let-7 is closely linked to cancer.

Seven cases of benign bone tumors, a case of spinal fusion and. seven rases of un-united fractures of shafts of long bones, were treated with a.llogenir bone matrix gelatin (BMG) implants and were followed up for more than one year. All tumor cases were cured by osteo-induction from BMG, while only three of the seven fractures united. Two of the failures were reimplanted with BMG and failed again. Not a single case developed post-operative infection nor immunologic reaction. The BMG used was obtained from the same preparation, so that deterioration of its biological activity by endogenous protease was not likely. BMG small BMG cubes were used in tumor cases and slabs in fractures. The small cubes . were mixed with pieces of gel-foam and parked into the post-operative bony cavities while the slabs were arranged layer by layer at the fracture sites. This might be one of the reasons why the tumor rases had higher rate of cure Another reason was that non-unions needed prolonged, continuous and firm internal and external fixation post-operatively while the tumor cases needed not The amount of BMP calculated from BMG was not enough for early and solid osteogenesis. In the authors'opinion, with the use of new biomaterial, it should be followed by new method of application, but the principles of surgery should not be changed

Objective To assess boron intake through food and drinking water for the people living in the boron industrial area.Methods Workers from boron mining and processing plants were selected as the exposure group,the other workers from the area where was near to the boron industrial area were selected as the community control group and peoples from the area where was far from boron industrial area were selected as the background control group,all the subjects were healthy adult men,Jul.2003-Sep.2004.Total 24 h food and drinking water of the subjects were collected,the boron concentrations and daily boron intake were analyzed.Results Boron concentrations of drinking water for the workers from boron mining area,community control group and background control group were 2.05,0.86 and 0.05 mg/L respectively;the food boron concentrations of the three groups were 9.46,6.19 and 3.29 mg/kg(DW)respectively;and daily boron intake through food and water were 8.00,4.25 and 1.40 mg/d respectively.The workers drinking the water from the staff canteen well and having dinner in this canteen had 95.5-469 mg/d boron exposure through food and water with average of 219.0 mg/L which was much higher than that of the other people and even closed to the LOAEL(lowest observed adverse effect level)of animals.Conclusion The risk of boron exposure for workers from boron mining and processing area is much higher than that of the other people.

Objective:To detect the expression of upstream stimulatory factor 1(USF1) and its tempo-spatial distribution during mice teeth development. Methods: Total protein was extracted from P1 and P11 d mice first molar teeth germ, and Western blot for USF1 was undertaken. Paraffin sections of first molar teeth germs from E13, 16, 19, P1, 5, 8, 11, 21 d and 6-month-old adult mice were prepared respectively and immunohistochemical staining was carried out. Results: Western blot analysis identified one Mr 43 000 protein from P11 d mice teeth germs, but none from P1d mice. Immunohistochemically, evidently positive staining for USF1 in mice teeth germs began from P5 d, and extended to P11 d, which was mainly confined to the cytoplasm of secreting ameloblasts and odontoblasts, but no staining in bud, cap and early bell stages of tooth germ. However, after tooth eruption on P21 d, USF1 became negative again, although it was still positive in the adjacent muscles, and the same result was observed in adult mice tooth. Conclusion: USF1 is expressed in tooth germ, which localizes solely in secreting ameloblasts and odontoblasts, and its expression was quite dynamic during mice tooth development.

Object To explore the beneficial effect of phytoecdysone (EDS) on myocardial infarction and its mechanism of action Methods Rat myocardial infarction model was prepared by ligating the left anterior descending coronary artery, and EDS was injected ip for seven consecutive days Serum creatine phosphokinase (CPK) glutamic oxalacetic transaminase (GOT), lactic dehydrogenase (LDH) activities, infarct size(IS), coronary blood flow, capillary vessel density and vascular endothelial growth factor (VEGF) expression were determined Results 0 5, 5, and 50 mg/kg of phytoecdysone were able to effect the activities of serum CPK, GOT, LDH in a dose depending manner with an optimal effect for improving cardiac zymogram at the dose of 5 mg/kg ip At this dosage EDS can markedly reduce IS, increase coronary blood flow, capillary vessel density and the expression of VEGF Conclusion ESD can alleviate myocardial infarction symptoms The mechanism of such beneficial effect may due to its ability to promote VEGF expression regeneration of capillary vessels and increase coronary blood flow

Objective To summarize the experience in diagnosis and management for the space occupying lesion of spleen. Method The clinical data of 29 cases treated by surgery were retrospectively analyzed. Results There were 15 patients with benign masses including 7 hamartomas, 5 hemangiomas, 1 pseudocyst, 2 tuberculoses of the spleen, and 14 with malignant tumors including 9 lymphomas, 3 angiosarcomas, 2 metastatic tumors in the spleen. Splenectomy was performed in all patients. All patients with benign masses survived except 2 patients lost follow up and 1 coexisting with hepataocellular carcinoma died half a year after the operation. Twelve of 14 patients with malignant tumor were followed up.Of them, 5 patients survived more than 5 years and 2 were alive 1 and 3 years after the operation respectively; 5 patients died 6 months to 4 years after the operation. Conclusions Ultrasonography and CT or MRI are the main means of diagnosis for the space occupying lesion of spleen.It is difficalt to make diagnosis of the splenic tuberculosis before operation.Splenectomy is a primary procedure of surgery.