Objective To investigate the effect of montelukast sodium chewable tablets joint Tongqiaobiyan particles in the treatment of children with allergic rhinitis.Methods 108 children with allergic rhinitis according to the methods were divided into A group and B group,54 cases in each group.Both A and B group were given Tongqiaobiyan particles,weight <25kg patients,each half bag(1g),3 times a day;weight >25kg patients,each bag, 3 times a day.At the same time the A group was given montelukast sodium chewable tablets:2 to 5 years old,each 4mg,once daily.6 to 14 years old,each 5mg,once daily.Period of treatment for two weeks.Total efficiency and snee-zing,nasal congestion,runny nose,itchy nose symptoms and dry cough improve time,the recurrence rate after one month,two months of the two groups were compared.Results After the treatment,94 cases were accomplished,falled off in 14 cases.Among them,the A group completed in 48 cases,falled off in 6 cases;The B group completed in 46 patients from,falled off in 8 cases.The total effective rate of the A group was 95.8%,which was significantly high-er than 78.3% in the B group(χ2 =6.513,P <0.05).The sneezing,nasal congestion,runny nose,nasal itching and dry caugh of the A group were relieved more significantly than the B group(t =2.628,3.30,2.74,3.58,3.09,all P <0.05).Followed up 1 month and 2 months,the recurrence rates of A group and B group were 6.5% and 20%, 15.2% and 25%,respectively,there were no significant difference between with the two groups(χ2 =1.122,1.338, all P >0.05).Conclusion Tongqiaobiyan particles jointly montelukast sodium chewable tablets integrative curative in treatment of allergic rhinitis can significantly improve clinical symptoms,and has no significant adverse reactions. This method should be popularized and applied.

With the development of global warming,urbanization,ageing of the population,it is paid more attention to the human health impacts on the frequent occurrence of heat wave and urban heat island effect.The study methods and advances in impacts of heat wave on human health were reviewed in the present paper.

Objective To investigate the effect of tissue adhesive glue in the repairment of eyelid lacerations and surgical incisions in children.Methods Retrospective comparison trial in paediatric ophthalmology department.Children who presented for laceration and surgical incision repair between November 2015 and October 2016 were evaluated.Patients from 11 months old to 12 years old with a total of 458 patients were randomly allocated to either glue (258 cases) or sutures (200 controls) to repair their wound.Cases and controls were similar in age,wound length and width involved.Those with wound seriously infected and longer than 5 cm were excluded.Length of time to perform procedure,pain assessment of procedure by doctor,parent and child and cosmetic outcome in 3 months and 1 year were measured.Results Time taken to repair the wound was faster in the glue group (P<0.01).Doctors and parents and the children themselves rated glue repair as less distressing (P<0.01).Complications (wound dehiscence,infection and corneal damage) were the same in both groups (P>0.05).Cosmetic outcome was better in the glue group in 3 months and 1 year (P<0.05).Conclusion Tissue adhesive glue is faster and easier and less painful than suturing.Tissue adhesive glue has better cosmetic outcomes than suturing when used for the repair of eyelid lacerations and surgical incisions in children.

Objective To verify the performance of the ADVIA Centaur XP automatic chemiluminescence analyzer produced by the Siemens company in quantitative detection of serum insulin (IRI) and C‐peptide(CpS) .Methods The blood samples from 40 in‐dividuals undergoing the physical examination were detected for calculating the imprecision and bias of IRI and CpS .The linear range of IRI was verified .The reference range of IRI and CpS was verified .Results The intra‐batch variable of coefficient of IRI and CpS was less than 6 .25% ,the inter‐batch imprecision were less than 8 .33% ;the bias of IRI and CpS detection was less than 12 .5% ;the linear range of IRI was 0 .50 - 307 .76 mIU /L ;the detection results of 40 serum samples showed that all IRS were with‐in the reference range provided by manufacturer ,the results in 2 cases of CpS was out of the reference range provided by manufac‐turer ,indicating that it conformed to the relevant requirements .Conclusion The main performance of the ADVIA Centaur XP auto ‐matic chemiluminescence analyzer for detecting IRS and CpS meets the requirements ,which could be used for clinical detection .

Objective To investigate the effect of lipoteichoic acid of bifidobacterium (BLTA) on the expressions of Fas/FasL in bearing melanoma B16 cancer mice. Methods Forty C57BL/6 mice were randomly divided into 4 groups (n=10),normal saline group (NS group),low-dose BLTA group (50 mg/L),middle-dose BLTA group (100 mg/L),and high-dose BLTA group (150 mg/L). Melanoma B16 cells (0.2 ml 5?109/L) were subcutaneously injected into the mice of the later 3 groups,and then,0.2 ml BLTA at corresponding doses was injected subcutaneously at the sites around the mass once a day for 10 d when the tumor (at least 2 mm?2 mm) was palpable. The NS group was given normal saline at same volume as in BLTA group. The changes of the killing activity of CTL was examined by MTT assay. The expression of the tumor-infiltrating CD4+ and CD8+ lymphocytes in tumor tissue were detected by immunohistochemistry. RT-PCR was used to detect the mRNA expression variance of the Fas and FasL in tumor and spleen tissue. The protein expression of Fas,FasL in tumor tissue and lymphocytes of spleen were detected by immunohistochemistry and Western blotting. Results Compared with the control group,BLTA significantly improved the CTL killing activity (P0.05). Conclusion BLTA enhances the killing activity of CTL by regulating Fas/FasL system,and thus,promotes apoptosis of tumor cells to suppress tumor immune escape in tumor-bearing mice,thereby it exerts anti-tumor effect further.

Objectives To study the impacts of air temperature and the other weather factors on the daily mortality of the residents in cities for targeting the preventive measures to decrease the excess mortality induced by climate change.Methods The daily mortality and weather surveillance data were provided by Chongqing Center for Disease Control and Prevention and China Meteorological Bureau respectively.The correlation between the weather factors(temperature,humidity,air pressure,rainfall,wind speed) and the daily mortality of residents in a county of Chongqing were analyzed by using the Poisson GAM of time series(increased by 1℃ for air temperate,decreased by 1% for relative humidity and one unit for air pollution index) adjusting for the secular trend,seasonal trend,short-term fluctuation and day of week.Results The excess mortality increased to 12% as the temperature increased 1℃.The excess mortality increased to 4% and 0.6% respectively as the relative humidity decreased 1% and the air pollution index increased one unit.Conclusion High temperature,with humidity and air pollution,will cause increased excess mortality of residents in this county

Tumor microvessel including two modes,as one is classic vascular structures surrounded with vascular endothelial cells and the other is vasculogenic mimicry( VM) surrounded with cancer cells.Recent studies suggested that anti-angiogenic drugs can inhibit classic microvessel structure significantly, but can not inhibit VM formation and even promote VM formation further more.This is one of the reasons affecting its clinical effica-cy.However,basic research has shown that traditional chinese medicine,gene therapy and drugs which is targeting for VM formation have an unique advantage in anti-VM.In this review, we summarize the progress of cancer therapy on targeting vasculogenic mimicry,and provide a basic for the development of new drugs for cancer thera-py through targeting both angiogenesis and VM formation.

[ ABSTRACT] Diabetes is characterized by an absolute or relative deficiency inβ-cell mass, which cannot be re-versed with existing therapeutic strategies.The restoration of the endogenous islet β-cells can stabilize the level of blood glucose.The isletβ-cells can be obtained from the directional differentiation of stem cells, but the process is complex and has the risk of teratomas generation.Cell direct reprogramming, one terminal differentiated cell can transdifferentiate into another kind of terminal differentiated cell, which is other than directional differentiation from stem cells.Direct reprogram-ming gives rise to the generation of isletβ-cells from one terminal differentiated cell, may be preferable for diabetes therapy because of its unique advantage.

Objective:To investigate the expression of CD69+ on lymphocyte and cytokine in active lymphocyte (CD69+) from patients with systemic lupus erythematosus( SUE) . So try to understand the lymphocyte activation, expression of cytokine in active lymphocyte and the pathogenesis of SLE.Methods: Expression of CD69+ on lymphocyte and intracellular cytokine(IL-2,TNF?) in active lymphoycte(CD69+) were investigated in 26 SLE and 15 healthy volunteers by using flow cytometric analysis and immunofluorescence staining method. Results:Expression of CD69+ on lymphocytes from active and inactive SLE patients were significantly higher than that of healthy controls( P

Aim To investigate the combined pharmacokinetic-pharmacodynamic(PK-PD) model of irbesartan in healthy Chinese male volunteers. Methods Eighteen healthy male volunteers received 300 mg irbesartan tablet orally. The plasma drug concentration (C_p) was determined by HPLC and pharmacologic effects, including SBP, DBP and HR, were measured simultaneously. The pharmakinetic and PK-PD model parameters were calculated. Results The pharmacokinetic profiles were best fitted a two-compartment open model. There was a hysteresis loop between effects and plasma concentrations. The relationship between effects and effect compartment concentrations of drugs could be represented by the Sigmoid-E_ max model. Conclusion We successfully eatablished the PK-PD model of irbesartan in healthy male volunteers. These findings may provide a more rational basis for patient-specific dosage individualization.