Objective:To compare the effect of misoprostol respectively with sublingual administration and rectal administration in the treatment of postpartum hemorrhage. Methods:Totally 72 pregnant women were randomly divided into two groups with 36 cases in each. The sublingual group was given misoprostol 600 μg under the tongue immediately after the front shoulder of baby was delivered, while the rectal group was given misoprostol 600μg into rectum deeply. The amount of blood loss in 2 hours and 24 hours after the de-livery and the adverse reactions were compared between the two groups. Results: The postpartum hemorrhageon in 2 hours and 24 hours of the sublingual group was significantly less than that of the rectal group, and the incidence of postpartum hemorrhage in the sub-lingual group was significantly lower than that of the rectal group, and the difference was statistically significant (P<0. 05). After the treatment, the incidence of adverse reactions between the two groups had no significant difference (P>0. 05). However, the incidence of shiver in the sublingual group was significantly higher than that in the rectal group, and the difference was statistically significant ( P<0. 05). Conclusion:The preventive effect of misoprostol with sublingual administration on postpartum hemorrhage after natural vagi-nal delivery is better than that with rectal administration, which is safe and convenient, and worthy of promoted application in clinics.

Objective To investigate the effects of ketamine on the effector functions (phagocytosis, respiratory burst and release of proteolytic enzymes) of lipopolysaccharide (LPS) stimulated human neutrophils in vitro. Methods Blood samples were collected from healthy volunteers. The study was divided into four groups: LPS group and three ketamine groups (K1, K2 and K3). The final concentration of ketamine in each group was 0, 3, 30 and 300 ?g/ml respectively. Phagocytosis was assessed in whole blood by NBT phagocytosis test (n=8) and respiratory burst by flow cytometry (with dihydrorhodamine 123 as fluorescent marker, n=5). The release of three proteolytic enzymes was measured with isolated polymorphonuclear neutrophils (PMNs) by turbidimetry (lysozyme) and chromatometry (elastase and ? glucurolidase) methods (n=9).Results Ketamine dose dependently inhibited phagocytosis, respiratory burst and proteolytic enzyme releasing of LPS stimulated human neutrophils in vitro. Higher concentrations of ketamine were required to suppress respiratory burst as compared with the concentrations needed to suppress phagocytosis and proteolytic enzyme releasing.Conclusions The inhibitory effects of ketamine on the effector functions of LPS stimulated human neutrophils may contribute to the attenuation of neutrophil mediated inflammatory injuries.

Objective To study the acute effects of PM2.5 on the heart rhythm of spontaneously hypertensive rats(SHR)and the mechanism.Methods Twenty-eight male spontaneously hypertensive rats(SHR)were randomly divided into four groups.PM2.5 was administered by intratracheal instillation at the doses of 0 mg/kg,7.5 mg/kg,15 mg/kg and 30 mg/kg respectively.ECGs were monitored at 30 min,1 h and 24 h later.Results The numbers of the rats with arrhythmia in all groups increased at 30 min after treatment.At 1 h after treatment,in control group the rats recovered,but in PM2.5 groups abnormal ECGs was still showed.However,ECGs of all groups became normal 24 h later.As shown by laser scanning confocal microscope(LSCM),the expression of Cx43 in the heart tissue of rats in 15 mg/kg and 30 mg/kg groups significantly decreased compared with the control group.There was no significant change in content of MDA and SOD in the heart tissue of PM2.5 treated rats.Conclusion PM2.5 exposure through inhalation may induce arrhythmia in SHR rats and the downregulated expression of Cx43 may play an important role in the pathogenesis.

Objective To evaluate safety and effect of percutaneous balloon mitral valvuloplasty(PBMV) for patients with rheumatic mitral stenosis and left atrial thrombi.Methods PBMV was performed in 27 patients with rheumatic mitral stenosis and left atrial thrombi. 19 cases of left atrial fresh thrombi revealed by transesophageal echocardiography (TEE) received warfarin orally for 3-6 months before PBMV. Results PBMV was successful in all cases of mitral stenosis and left atrial thrombi. Left atrial fresh thrombi was completely resolved in 9 cases and became smaller chronic organized thrombi in 10 cases after warfarin anticoagulation treatment among 19 cases of left atrial fresh thrombi revealed by TEE. In 5 cases of left atrial chronic organized thrombi shown only transthoracic echocardiography and without anticoagulation treatment, one case had cerebral embolism. No complication occurred in other cases.Conclusions The study showed that patients with rheumatic mitral stenosis and atrial fibrillation should have routine TEE. PBMV for rheumatic mitral stenosis with left atrial thrombi after anticoagulation treatment is safe and effective.

Objective To investigate the cell cycle of airway smooth muscle cells(ASMC) in asthmatic rats with airway remodeling and to explore a new method for the prevention and treatment of asthma.Methods The asthmatic rat models were established.The pathologic changes of inner airway wall and ASMC were determined by observing and analyzing the lung tissue sections stained with HE,and the cell cycle distribution of ASMC were analyzed by flow cytometry.Results The inner airway wall and smooth muscle layer were much thicker in rats of asthma group than those of control group(P

OBJECTIVE:To study the preparative methods of the long-circulating solid lipid nanoparticles(LSLN)carrying ginkgolides A and B(GAB)and to study the physicochemical characteristics of the GAB-LSLN.METHODS:GAB-LSLN was prepared by ultrasonication or high pressure homogenization.Transmission electron microscopy was employed to study its shape.Particle size,zeta potential,and entrapment efficiency of GAB-LSLN were determined,and its stability after storage under room temperature for 4 weeks was determined as well.RESULTS:The GAB-LSLN prepared by ultrasonication was platelet-shaped and irregular,and that prepared by high pressure homogenization was spherical and regular in shape.The particle diameters of GAB-L SLN prepared by ultrasonication and high pressure homogenization were(219.6?14.3)nm and(173.9?10.4)nm respectively(P0.05).CONCLUSION:High pressure homogenization is superior to ultrasonication in that the prepared GAB-LSLN has small particle size,high stability and high entrapment efficiency.

Objective To investigate the clinical effects of Metoprolol Tartrate Tablets and Sodium Fructose Diphosphate(FDP) :injection in the early treatment of 48 cases of acute myocardial infarction(AMI) patients with thrombolytic therapy.Methods 84 cases of acute myocardial infarction who were in accordance with the indications for thrombolytic therapy in patients were randomly divided into a treatment group(group A,48 cases,treated with Metoprolol Tartrate Tablets and Sodium Fructose Diphosphate Injection) and a control group(group B,36 cases,treated with the conventional therapy).The occurrence of reperfusion arrhythmias,early sighs and symptms,and retoration of myocardial injury were observed in patients after thrombolytic therapy in order to understand clinical effect on the patients with that treatment.Results In treatment group there were decreased incidence of heart symptoms (chest pain,cardiopalmus) and RA(group A,36.1%;group B,71.4%),improved recovery of cardiac muscde enzymogram as compared to control,which was significantly different with control and between treatment groups (P

OBJECTIVE To investigate the resistance of clinical isolated strains to the commonly used antibacterials in our hospital 2007-2008.METHODS Clinical isolated strains and sensitivity of drugs were detected by ATB system.The result of drug sensitivity was judged by CLSI standard and analyzed with statistical software WHONET5.3.RESULTS Altogether 3150 strains bacteria were isolated,17.4% were Gram-positive strains and 82.6% were Gram-negative strains,and the top five isolates were Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii,and Staphylococcus aureus.The reasistance rate of Gram-positive strains to minocycline was 15.4%.Five VRE strains were isolated.Various Enterobacteriaceae bacteria were sensitive to imipenem meropenem,cefoperazone-sulbactam and piperacillin-tazobactam,and their rate was 86.5% to 97.7%.Some of Acinetobacter and Stenotrophomonas maltophilia were multidrug resistant.CONCLUSIONS It is serous that multidrug resistance of isolated strains of the patients exists in our hospital.

Objective To investigate the effects of atorvastutin on the levels of plasm imflammation markers (hs - CRP, MMP - 3, TNF - α) and intima - media thickness(ITM) of the common carotid artery in patient with hyperlipidemic and atherosclerosis. Methods Fifty patient with hyperlipidemic and atherosclerosis were treated with atorvastatin (10mg qd) for 12 weeks. The levels of TC, TG, LDL -C, HDL -C, hs -CRP, TNF -α and MMP -3 were detected with radioimmmuneassay and eolorimetric methods. Color Doppler echo-cardiography was used to measure intima -media thickness(ITM) of the common carotid artery after 6 weeks and 12 weeks. Results TC, TG, LDL -C, hs -CRP, TNF -α and MMP -3 were decreased significantly(P < 0.01)after 12 weeks. IMT was decreased signifi-cantly(P < 0.05) and HDL - C was increased significantly (P < 0.05) after 12 weeks. Conclusion Atorvastatin could be effective in re-ducing blood - fat and anti - inflammatory action , regulating blood vessel endothelium (BVE) function and reversing structural chan-ges in IMT of the common carotid artery.

Objective This study aimed to discuss the different assessment of pain among medical and nursing staff and cancer patients and supply reference for proper analgesic precept for clinical application and nursing measures. Methods We collected the assessment of psychological pain and physiological pain by 55 hospitalization cancer patients, 40 physicians in-charge and 55 nurses in-charge in one week by Johnson inventory. The assessment results were compared and at the same time the relevant problems of the attitude to cancer pain by patients was also investigated. Results Improper recognition existed in cancer pain treatment by most cancer patients. The physiological pain was higher than the psychological pain assessed by both patients and nurses (P<0.05). But the pain assessment by patients was higher than that by the nurses (P<0.05). The assessment of psychological pain was higher than the physiological pain by doctors and both aspects were lower than those by patients, but no statistical difference was seen (P>0.05). The assessment by doctors was more accurate than that by nurses. Conclusions Routine establishment of pain assessment inventory for patients could instruct patients how to record the degree of their pain. We should strengthen the standard training about pain management knowledge and give timely communication with patients' cancer pain.