Objective　To study the MRI diagnosis of spinal metastasis.Methods　The MRI appearances of 128 cases of spinal metastasis proved clinically were retrospectively analyzed.Results　(1)The MRI signal was T1WI low in 87.5% and 12.5% was mixed or equal.(2)Multi-focus in 80.5%.The "jump up sign"occurred in 52.3%,the accessory parts destructed in 64.1%,in which root of vertebral destructed with expanded in 57%.(3)The masses of paravertebral occurred in 31.3%.The disc of vertebral was not destructed.(4)The patients associated with vertebral compresion fracture such as "wedge","disc"or"wedge of rear"in 52.3%.Conclusion　The MRI findings of spinal metastasis is of high sensitivity and spectivity.The method is advanced than X-ray and CT.

Nitroxide radicals are a kind of stable organic free radicals. Due to the presence of N-O· and unpaired electrons in its structure, it has many characteristics, and thus can be used as a spin marker to explore the mechanism of biological reactions; with its magnetic properties, it can be used for the development of multifunctional magnetic molecular materials and used as a polymerization inhibitor and catalyst in organic reactions. More importantly, it has a variety of biological activities such as anti-oxidation and anti-tumor, and so has attracted much attention in the research and development of new drugs. For example, the spin labeling of nitroxide radicals on anticancer drug podophyllotoxin can enhance the efficacy and reduce the toxicity, and can be easily to be absorbed by the body, thus obtaining a new anti-cancer drug 4-[4″-(2″,2″,6″,6″-tetramethyl-1″-piperidinyloxy nitroxide radical) amino]-4′-demethyl epipodophyllotoxin(GP-7). It is an effective way to seek new drugs by introducing pharmacophore to modify nitroxide radicals or it can be spin-labeled on active natural products to obtain new compounds with high efficiency and low toxicity. The research progress of derivatives and its biological activitives of nitroxide radicals are summarized, aiming to provide theoretical basis for the developing and utilizing of nitroxide radicals and searching for new drugs.

OBJECTIVETo study the chemical constituents of Periploca forrestii.

METHODThe constituents were separate using such various column chromatographic techniques as silica gel, RP-18 silica gel, MCI and Sephadex LH-20. Their structures were identified by such methods as spectral analysis.

RESULTTen compounds were isolated and identified as periforgenin A-3-O-beta-digitoxopyranoside (1), beta-sitosterol (2), periforoside I (3), ursolic acid (4), periplogenin (5), periplocin (6), glycoside E (7), periplocoside M (8) , daucosterol (9), 2alpha, 3alpha, 23-trihydroxy-urs-12-en-28-oic acid (10).

CONCLUSIONCompound 1 was a new cardiac glycoside and compound 8 was reported for the first time from this plant.

Cardiotonic Agents ; chemistry ; isolation & purification ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Molecular Structure ; Periploca ; chemistry

OBJECTIVE: To explore the polymorphisms of T149C and T950C gene in osteoprotectin (OPG) promoter sites and the levels of serum OPG and soluble nuclear factor-ΚB receptor activator ligand (sRANKL) and the incidence of coronary heart disease (CHD). METHODS: 528 patients in Tianjin suspected of CHD and underwent coronary angiography (CAG) who admitted to the department of cardiology of Tianjin Chest Hospital from April 2017 to December 2018 were enrolled. According to the CAG results, they were divided into two groups: CHD group (n = 302) and non-CHD group (n = 226). The gender, age, history of hypertension, family history of CHD, diabetes, levels of blood lipid parameters in serum and other clinical data of patients were recorded. The levels of serum OPG and sRANKL were measured by enzyme-linked immunosorbent assay (ELISA). T149C and T950C gene polymorphisms were analyzed by polymerase chain reaction-restriction endonuclease fragment length polymorphism (PCR-RFLP) methods. Hardy-Weinberg genetic balance test was performed for alleles. Binomial classification multivariate non-conditional Logistic regression method was used to analyze the relationship between T149C and T950C gene polymorphisms, serum levels of OPG and sRANKL and CHD. RESULTS: All patients were enrolled in the final analysis. The serum level of OPG in CHD group was significantly higher than that in non-CHD group (μg/L: 1.76±0.49 vs. 1.47±0.29, P < 0.01), the serum level of sRANKL was significantly lower than that in non-CHD group (ng/L: 342.14±121.38 vs. 376.63±108.66, P < 0.05). Logistic regression analysis showed that after adjusting for age, gender, blood lipid parameters, diabetes and other factors, the increase in serum OPG level was an independent risk factor for CHD [odds ratio (OR) = 1.995, 95% confidence interval (95%CI) = 1.935-2.066, P = 0.012]. PCR-RFLP results showed that TT, TC and CC genotypes were found in T149C and T950C of OPG promoter. According to Hardy-Weinberg equilibrium test, the polymorphisms of OPG T149C and T950C accorded with Hardy-Weinberg law, achieving genetic balance with representative of the population. The frequencies of TT, TC, CC and alleles T and C in T149C genotypes of non-CHD group were 53.5%, 42.9%, 3.6%, 75.0% and 25.0%, respectively, and they were 43.1%, 50.3%, 6.6%, 68.2% and 31.8%, respectively in CHD group. There were statistically significant differences in genotype and allele frequencies between the two groups (all P < 0.05). It was shown by Logistic regression analysis that the risk of CHD in TC+CC genotype of T149C was 1.86 of TT genotype (OR = 1.86, 95%CI = 1.24-2.78, P = 0.003). It was suggested that C allele might be a susceptible gene for CHD. In non-CHD group, the frequencies of TT, TC, CC, and alleles T and C in T950C genotypes were 39.8%, 46.5%, 13.7%, 63.1% and 36.9%, respectively. They were 39.4%, 43.4%, 17.2%, 61.1% and 38.9%, respectively in CHD group. There were no significant differences in genotype and allele frequencies between the two groups (all P > 0.05). Logistic regression analysis showed that TC+CC genotype of T950C was not related with CHD. CONCLUSIONS The increased level of serum OPG was closely related with CHD and could be used as a risk factor for CHD. The cases carried OPG T149C TC+CC genotype might have the risk suffering CHD. C allele is might be a susceptible gene.
China/epidemiology* ; Coronary Disease/epidemiology* ; Female ; Humans ; Male ; Osteoprotegerin/genetics* ; Polymorphism, Genetic ; Promoter Regions, Genetic/genetics* ; Risk Factors