Objective To investigate the correlation value between the impression depth of umbilical cord around neck and the blood flow indexes of middle cerebral artery (MCA) and umbilical artery (UA) in fetus.Methods Totally 139 late pregnancy fetuses of umbilical cord around neck and 28 normal fetuses were enrolled in the study.The systolic maximum velocity/diastolic minimum velocity (S/D),resistence index (RI),pulsatility index (PI) of MCA and UA in fetuses were measured by Doppler ultrasound,and the correlation of the pressure trace depth with MCA and UA blood flow parameters were analyzed.Results The pressure trace depth of umbilical cord around neck and the S/D,RI,PI of MCA were positively correlated (one round of umbilical cord around neck,r=0.32,0.32,0.35,two or more rounds of umbilical cord around neck,r=0.71,0.69,0.53,all P＜0.05).The pressure trace depth of umbilical cord around neck and the S/D,RI,PI of UA had no correlation.Compared with normal fetuses,the parameters of UA had no significant differences in fetuses with umbilical cord around neck (all P＞0.05).The S/D,RI,PI of MCA in fetuses with pressure trace depth ≥0.80 cm and fetuses with two or more rounds of umbilical cord around neck were higher than those of normal fetuses (all P＜0.05).Fetuses with two or more rounds of umbilical cord around neck had obviously increased cesarean rate compared with normal fetuses.Compared with normal fetuses,the fetuses with pressure trace depth ≥0.80 cm had increased abnormal fetal heart rate,prolonged labor time during the second stage (all P＜0.05).Conclusion In late pregnancy fetuses with umbilical cord around neck,the blood flow parameters of MCA are more sensitive than those of UA.The pressure trace depth ≥ 0.80 cm may lead to intrauterine fetal anoxia.

Objective To explore the significance of transvaginal color Doppler ultrasound in the diagnosis of cervical carcinoma. Methods 45 patients undergoing surgery for cervical carcinoma were enrolled. Transvaginal color Doppler flow imaging was performed preoperatively to detect the arterial blood flow signals within the tumors and the lowest resistance index (RI) value was recorded for analysis. Results Significant difference RI values were noted in cervical carcinoma group compared with contrast group (P <0.001). RI were associated with tumor diameter, stage status, pathologic grade and pelvic lymph node metastasis of cervical carcinoma(P < 0.05). There were no relations between RI and patients's age and pathologic type (P >0.05). Conclusion The angiogenesis character of cervical carcinoma can be showed by RI, and RI had some inherent connections with clinicopathologic parameters of cervical carcinoma. Transvaginal color doppler ultrasound could accurately predict angiogenesis of cervical carcinoma preoperatively and be of importance in the diagnosis of cervical carcinoma. It could provide standard in prognosis evaluation.

Objective:To investigate the effect of chronic alcohol consumption on both left ventricular myocardial collagen and diastolic function in rats,and their relationship thereof.Methods:Twenty-four male Wistar rats were randomly divided into 2 groups:control group(n=12)and ethanol group(n=12).The changes in cardiac diastolic function were evaluated by echocardiography and tissue Doppler imaging(TDI).The value of myocardial hydroxyproline content was determined by hydroxyproline reagent kit.The expressions of collagen Ⅰ and collagen Ⅲ mRNA were detected by RT-PCR analysis.Results:It was found that mitral E and mitral annulus Ea were decreased,mitral annulus Aa was increased,and isovolumic relaxation time(IVRT)was prolonged in the ethanol group compared with those in control group(P＜0.05).The value of Ea/Aa ratio was greater than 1 in control group and less than 1 in ethanol group(P＜0.01).It was found that myocardial hydroxyproline content,collagen Ⅰ,collagen Ⅲ mRNA expression and their ratio significantly increased in ethanol group compared with those in the control group(P＜0.01).There was positive correlation between hydroxyproline content,collagen Ⅰ,collagen Ⅲ mRNA expression,and collagen Ⅰ /collagen Ⅲ mRNA ratio with IVRT(P＜0.05),and negative correlation between hydroxyproline content,collagen Ⅰ,collagen Ⅲ mRNA expression,and collagen Ⅰ /collagen Ⅲ mRNA ratio with the Ea/Aa ratio(P＜0.01).Conclusion:Chronic ethanol consumption can induce increase in left ventricular myocardial collagen synthesis and impairment in diastolic function in rats.Left ventricular diastolic dysfunction correlates with increase in myocardial collagen synthesis positively.

Objective To evaluate the efficacy and adverse reactions of CT-guided 125I radioactive seed implantation in treatment of locally recurrent rectal cancer (LRRC).Methods Thirty patients with LRRC who refused operation or were unable to endure pelvic radiotherapy received 125I seed implantation under CT guidance.Three-dimensional treatment planning system was used to calculate the number,activity,and dose of the seeds needed.The activity of seeds ranged from 14.8 to 29.6 MBq with a median of 25.9 MBq,the seed numbers ranged from 33 to 137 with a median of 74.5,the prescription doses ranged from120-160 Gy,and the actual verification dose D90 ranged from 75.91 to 159.32 Gy with a median of 119.77 Gy.Dosimetric verification by CT scanning was conducted immediately after the treatment.Follow-up was conducted for 15.2 months(4.2-35.0 months).Results The follow-up rate was 93.3％.The pain relief rate was 95.2％.The overall response rate was 50.0％,including a complete response rate of 13.3％ and a partial response rate of 36.7％.The 1-and 2-year local control rates were 30.0％ and 8.0％ respectively.The median local control survival time was 7.8 month.The 1-and 2-year survival rates were 66.5％ and 32.9％ respectively.The median overall survival time was 21.5 months.Complications,mainly adverse effects of skin and urinary system (frequent urination,urgent urination,and dysuria) occurred in 6 patients with a rate of 20.0％.Conclusions Minimally invasive and with satisfying efficacy and tolerable complications,CT-guided 125I radioactive seed implantation is a favorable option for treatment of LRRC,especially for the patients who have undergone previous pelvic radiation.

Objective:To investigate the neural mechanism of long-term exposure to high altitudes environment on the processing stage of perceptual closure.Methods:A cross-sectional study was conducted. Thirty college students who first entered the plateau area (at an altitude of 3 658 m) and had lived in high-altitude areas for two years were selected as high altitude group (HA group), and 29 college students matched in age, gender and education level who had never been to the plateau area were selected as sea level group (SL group). The differences of basic physiological parameters were compared between the two groups.The face pairwise comparison paradigm was applied in the subjects of the two groups and the differences between the two groups were compared by event-related potentials (ERPs) technology. SPSS 22.0 software was used for statistical analysis, and the mean ± standard deviation was used for statistical description. Independent sample t test was used for comparison of physiological data between the two groups, and repeated measurement variance analysis was used for ERP data. Results:The physiological results revealed that the HA group had a higher pulse rate((86.71±10.82)/min, (75.97±11.28)/min ; t=-3.19, P=0.002) and diastolic blood pressure than the SL group ((75.93±9.19)mmHg, ( 68.59±11.42) mmHg ; t=-3.20, P=0.002). The oxygen saturation level was significantly lower than that of SL group ((90.77±2.25)%, (98.31±1.56) %) ; t=14.00, P<0.001). In the face pairwise comparison paradigm test, the main effect of P1 latency of ERP was significant.Compared with SL group, HA group showed significantly shortened latency of P1 ((105.10±15.59) ms, (128.35±14.40)ms, P<0.001). The main effect of group of N170 amplitude was significant, HA group was larger than that of SL group((-7.57±3.83) μV, ( -5.11±3.26) μV, P=0.005). The interaction effect between group and hemisphere of NCL amplitude was significant ( F(1, 57)=9.72, P=0.003). Simple effect test showed that the amplitude on the right hemisphere of SL group was significantly larger than that on the left hemisphere((0.46±1.31) μV), (1.16±1.33) μV ). The latency of P1 was significantly correlated with heart rate( r=-0.46, P<0.01), oxygen saturation level( r=0.64, P<0.01) and diastolic blood pressure( r=-0.26, P=0.049). Conclusion:After long-term exposure to high altitude, the neural response related to early visual perception is faster. More psychological resources are recruited during the face structural encoding stage. Contra-lateral compensating effect appeares in the stage of perceptual closure.

In order to determine the pathophysiologic significance of electrocardiographic ventricular hypertrophy in athletes (LVH ath), echocardiographic measurements of left ventricular mass (LVM) were performed on 50 LVH ath. and 50 non-LVH matched ath. They were members of National Teams of track and field, swimming, football and cycling, with an average age of 20.74?3.3 years. Anatomic validation of the method was used for calculation of LVM in this study. Comparisons of LVM were made between LVH ath. and non-LVH ath., and between non-LVH ath. and the untrained:LVM=1.04?[(LVID+PWT+IVST)~3-(LVID)~3]-14Results showed no significant difference of LVM between LVH ath. and non-LVH ath. while LVM of non-LVH ath. were considerably greater than that of the untrained (P

Objective To explore the clinical effects of oxygen-driven atomized inhalation of recombinant human inter-feron-α2a injection in the treatment of infantile capillary bronchitis. Methods 240 infant patients with capillary bron-chitis were assigned to a control group and a treatment group according to their disease conditions, with 120 patients in each group. The control group received regular treatment(oxygen uptake, atomized inhalation of pulmicort respulas+nebulized terbutaline sulphate solution used for anti-inflammation and antiasthma, aspiration of sputum, antibiotics giv-en to infants suspected to be complicated with bacterial infection, intravenous drip of methylprednisolone given to in-fants with severe symptoms, etc.); The treatment group further received oxygen-driven atomized inhalation of 600,000 international units of recombinant human interferon-α2a injection, once a day for 5 days. Clinical effects and main ob-servational indices between the two groups were compared. Results Total effective rate was 95.0% in the treatment group and 78.3%in the control group, and the differences between the two groups were statistically significantly(P<0.05). Alleviating time of asthma, disappearing time of lung rale, time of persistent cough and hospitalization time in the treatment group were all lower than those in the control group, and the differences between the two groups were statisti cally significant (P<0.05). Significant adverse events were not detected during the treatment period of oxygen-driven atomized inhalation of recombinant human interferon-α2a injection applied by the infant patients. Conclusion Oxygen-driven atomized inhalation of recombinant human interferon-α 2a injection in the treatment of infantile capillary bron-chitis has an exact curative effect, and it is safe, which is worthy of clinical pediatric promotion and application.

Objective To investigate the relationships between the expression levels of tumor necrosis factor receptor associated factor 4 (TRAF4) and ribosomal S6 protein kinase 4 (RSK4) protein in gastric cancer tissues and the recurrence after laparoscopic radical gastrectomy. Methods In total, 176 patients were divided into the recurrence and non-recurrence group, and the expression levels of TRAF4 and RSK4 protein in cancer and adjacent tissues and in gastric cancer tissues in the recurrence and non-recurrence group were compared. The influencing factor of recurrence and the efficacy of TRAF4 and RSK4 protein expression in predicting recurrence were analyzed. Results The positive expression rate of TRAF4 protein in gastric cancer tissues was higher than that in adjacent tissues (P < 0.05) and that in the recurrence group was higher than that in the non-recurrence group (P < 0.05). The positive expression rate of RSK4 protein in gastric cancer tissues was lower than that in adjacent tissues (P < 0.05) and that in the recurrence group was lower than that in non-recurrence group (P < 0.05). The largest tumor diameter 5 cm, poor differentiation, TNM Ⅲ stage, depth of invasion T3-T4, lymph node metastasis, absence of adjuvant chemotherapy after operation, positive expression of TRAF4 and RSK4 protein, and regular diet w influenced the post-operative recurrence (all P < 0.05). The accuracy of TRAF4 and RSK4 protein in gastric cancer tissues in combined predicting the recurrence was 83.52%. Conclusion The expression of TRAF4 protein is high, and the RSK4 protein is low in gastric cancer tissue, which are related to recurrence.

Nitroxide radicals are a kind of stable organic free radicals. Due to the presence of N-O· and unpaired electrons in its structure, it has many characteristics, and thus can be used as a spin marker to explore the mechanism of biological reactions; with its magnetic properties, it can be used for the development of multifunctional magnetic molecular materials and used as a polymerization inhibitor and catalyst in organic reactions. More importantly, it has a variety of biological activities such as anti-oxidation and anti-tumor, and so has attracted much attention in the research and development of new drugs. For example, the spin labeling of nitroxide radicals on anticancer drug podophyllotoxin can enhance the efficacy and reduce the toxicity, and can be easily to be absorbed by the body, thus obtaining a new anti-cancer drug 4-[4″-(2″,2″,6″,6″-tetramethyl-1″-piperidinyloxy nitroxide radical) amino]-4′-demethyl epipodophyllotoxin(GP-7). It is an effective way to seek new drugs by introducing pharmacophore to modify nitroxide radicals or it can be spin-labeled on active natural products to obtain new compounds with high efficiency and low toxicity. The research progress of derivatives and its biological activitives of nitroxide radicals are summarized, aiming to provide theoretical basis for the developing and utilizing of nitroxide radicals and searching for new drugs.

Objective To examine the anticancer effect of a novel histone deacetylase inhibitor (HDACi), JZ004, on colon cancer cells HCT-8 and HT-29, and to investigate the molecular mechanisms of proliferation inhibition and apoptosis induction of cancer cells treated by JZ 004.Methods Colon cancer cells were treated with a series of concentrations of JZ004 .MTT assay was used to detect the proliferation of cancer cells .The cell cycle distribution and apoptosis were deter-mined by flow cytometry .Rhodamine 123 and DCFH-DA were applied to detect the mitochondrial membrane potential (ΔΨm) and reactive oxygen species ( ROS) production.The protein expressions of acetyl-histone H3, p21, cyclin-dependent kinase(CDK)4, Bcl-2, Mcl-1 and Bax were assayed by Western blotting .Results JZ004 was found to inhibit proliferation and induce apoptosis of colon cancer cells in a time-and dose-dependent manner , accompanied by a dose-dependent hyperacetylation of histone H3.JZ004 induced the cancer cell arrest in G 0/G1 phase by increasing the expres-sion level of p21 while CDK4 was downregulated .JZ004 also increased cellular ROS production and reduced ΔΨm by regu-lating the expressions of Bcl-2 family proteins .Conclusion As a novel HDACi , JZ004 effectively inhibits proliferation and increases ROS production to induce apoptosis of colon cancer cells .The results indicate that JZ004 is a potential compound to be developed as an anti-colon cancer agent for clinic application .