AIM: To observe the relationship between the reversal effect of 4 alkaloids of TCM on expression of P170 being obtained multi-drug resistance to mouse S180 tumor cell induced by chemotherapy protocol of PFC and the adjustment of the cell apoptosis.And to discuss the difference among alkaloids of TCM on reversion of being obtained multi-drug resistance in clinic.To improve the curative effect thereby. METHODS: By the methods of less dosage of Chemotherapy PFC,to set up the mouse models of multi-drug resistance of S180 tumor cell.At the same time to give the mouse matrine,terandrine,oxymatrine and berberine hydrooh loride for 2 weeks and then to observe the P170,TOPOⅡ,Fas and apoposis by flow cytometry. RESULTS: Matrine and terandrine could obviously reduce the expression of P170,the activation of TOPOⅡ induced by Chemotherapy and increase the ratio of the express of Fas and the apopsis of drug resistance to tumor cell.And berberine hydrochloride could obviously reduce the expression of TOPOⅡ. CONCLUSION: The adjustment to the apoposis of tumor cell by matrine and terandrine is related to the reversion of being obtained multi-drug resistance to tumor cell' expression,besides the different degree

The fourth time national survey of Chinese materia medica (CMM) was used. The quadrat survey contained recording on species and amounts of plants, collection of specimens and CMM. The weight of main medicinal plant was measured. The quadrat numbers were calculated on regions, plant species and sea level of samples from different regions. There were 703 species of medicinal plants. The 143 species of main medicinal plants, which occupied to 74.5% in main medicinal plants in Chongqing, belonged to 71 families and 132 genera. There were 128 species of wild main medicinal plants. The 30 species of main medicinal plants were cultivated (including 15 species of both wild and cultivated medicinal plants). Among them, 14 species were in large-scale cultivation. This investigation helped the understanding of present situation of medicinal species distribution in Chengkou County, the habitats of wild medicinal plants, and the resources reserves, which provided references for the continuous development of Chinese herbal medicine resources.

Objective: To determine the national norm of Chinese questionnaire of quality of life in Chinese patients with cardiovascular diseases (CQQC) according patients with hypertension, coronary artery disease（CAD）, and congestive heart failure（CHF）and to analyze the influence factors. Methods: The quality of life was estimated by CQQC to evaluate 7937 persons normal and with cardiovascular diseases（contain with hypertension, CAD, and CHF ） in 28 hospitals of 19 cities across China. The data were compared in gender, age, exercise, psychologic condition, education, number of family member and medical cost. Results: 1、The mean scores（national norms）of normal persons were （95.74±23.21）; 2、The scores of hypertension patients were （73.75±23.62）, the scores of hypertension stage 3 group （69.06±21.78）were least compared with other stages（P<0.01）; 3、The scores of CAD patients were（64.67±20.59）, the scores of unstable angina group（55.37±18.62）were least compared with other types in CAD patients（P<0.01）; 4、The scores of CHF patients were （40.36±17.24）, it was least compared with those of hypertension and CAD patients （P<0.01）; 5、Influence factor: （1）Sex, age: The mean scores were （71.41±27.8）for 7937 persons, the scores of male persons were significantly higher than those of female (P=0.000). In normal persons, along with age increased, the scores decreased （P<0.05） ; except ≥70 ages group, the scores of male persons were higher than those of female（P<0.05） ; （2）Exercise: The scores of patients participating exercise were significantly higher than those of no participating exercise [（72.64±21.87）vs.（66.91±23.13）, P=0.000]; （3）Psychological condition: The scores of patients with healthy psychological condition were significantly higher than those of with psychologic disorder [（70.13±25.66）vs.（68.91±20.44）, P=0.001]; （4）Other: patients with better education, more than 1 family members and medical insurance or public expense had more scores (P<0.05～0.001). Conclusions： The sample size is large and the datum is reliable for Chinese questionnaire of quality of life in Chinese patients with cardiovascular diseases. It can reflect influence of age, sex, exercise, psychologic condition, family, education background, medical condition and disease, its item is brief, clear, and operation is easy, so it may well be spread.

Objective To establish a magnetic nanoparticles separation-based quantitative real-time PCR(RT-PCR)assay for fast and accurate detection of Plasmodium falciparum and providing a technical support for improving the control and preven-tion of imported malaria. Methods According to the conserved sequences of the P. falciparum genome 18SrRNA,the species-specific primers and probe were designed and synthetized. The RT-PCR was established by constructing the plasmid standard , fitting the standard curve and using magnetic nanoparticles separation. The sensitivity and specificity of the assay were evaluat-ed. Results The relationship between the threshold cycle(Ct)and logarithm of initial templates copies was linear over a range of 2.5×101 to 2.5×108 copies/μl(R2=0.999). Among 13 subjects of entry frontier,a P. falciparum carrier with low load was de-tected by using the assay and none was detected with the conventional examinations(microscopic examinations and rapid tests). Conclusion This assay shows a high sensitivity in detection of P. falciparum,with rapid and accurate characteristics,and is especially useful in diagnosis of P. falciparum infectors with low parasitaemia at entry-exit frontier ports.

OBJECTIVETo study the dynamic accumulation of the effective components and biomass of Coptis chinensis, so to provide the experimental date of optimal harvest time for C. chinensis in Hongya county.

METHODThe samples of three to five years were gathered from the same field and time. The biomass was analyzed by weighed. The jatrorrhizine, columbamine, epiberberine, coptisine, palmatine and berberine in C. chinensis were analyzed by HPLC.

RESULTWith the increasing of years of growth, the rootstalk biomass of C. chinensis was increasing continuously. The biomass growth of four-year-old C. chinensis was the fastest in the year. From September to October was the fastest season of the growth of rootstalk. The dynamic accumulation in rootstalk C. chinensis had regularity in the certain extend. The contents of six alkaloids and all alkaloids in 4-year-old C. chinensis were more than that in 3-years-old and 5-year-old. The contents of six alkaloids were mostly highest in August. From July to December, there is no significant difference in the contents of columbamine, epiberberine, coptisine, palmatine, berberine and all alkaloids in 4-years-old C. chinensis.

CONCLUSIONAccording to the biomass and the accumulation pattern of the effective components in the C. chinensis, the optimal harvest time is from September to October of 4-year-old C. chinensis.

Alkaloids ; analysis ; Biomass ; Coptis ; chemistry ; metabolism ; Seasons

OBJECTIVETo establish seedling classification criteria of Coptis chinensis.

METHODThe height of plant, leaf number, leaf length, leaf wide, weight of leaf, weight of root were measured, the main measurement indexes of seedlings of C. chinensis were chosen through correlation and regression analysis. The seedling classification criteria were formulated by dynamic clustering analysis.

RESULTThe criteria of the 1st-grade seedlings were as follows: leaf number above 8 leaves, height of plant between 12 and 14 cm. The criteria of the 2nd-grade seedlings were as follows: leaf number between 6 and 8 cm, height of plant between 9 and 12 cm. The criteria of the 3rd-grade seedlings were as follows: leaf number between 4 and 6 cm, height of plant above 9 cm.

CONCLUSIONThe seedling classification criteria of C. chinensis was scientific and feasible, and can be used for the quality control standard of C. chinensis.

China ; Cluster Analysis ; Coptis ; classification ; Medicine, Chinese Traditional ; Plant Leaves ; classification ; Plant Roots ; classification ; Plants, Medicinal ; classification ; Seedlings ; classification