Objective To study the clinical significance by detecting the levels of sera cystatin C(Cys C)in patients with colorectal cancer.Methods We compared the levels of sera cys C detected with the immune rate nephelometry(IRN)in 71 colorectal cancer patients.Then we compared it in 40 healthy persons and 48 colorectal disease patients without cancer to investigate the relationship between the level of cys C and the clinicopathological characteristics.Results The levels of cys C in colorectal cancer patients were much higher than those in the other control groups,and were significantly related to Dukes stages and the tumor differentiated degree.Conclusion The results provide convincing evidence that cys C may contribute to occurrence and development of colorectal cancer.

Objective To develop an HPLC method for the simultaneous determination of 7-ethyl-10-hydroxycamptothecin-10-palmitic acid ester(SN38-PA)and its active metabolite 7-ethyl-10-hydroxycamptothecin(SN38)in rat plasma. Methods The inter standard was 10-hydroxycamptothecin. The protein in plasma was precipitated with methanol after acidification with formic acid. SN38-PA and SN38 were separated on Agilent C18 column(4.6 mm×250 mm,5μm) with gradient elution by using the mobile phase of methanol-0.2% formic acid solution. The flow rate was 1 ml/min. The detection wavelength was set at 372 nm. The column temperature was maintained at 30℃. Results The linear ranges for SN38-PA and SN38 were 0.25-62.5(r=0.9998) and 0.05-12.5 μg/ml (r=0.9997) respectively. The limits of quantification were 0.18 and 0.04 μg/ml, respectively. The average relative recovery of SN38-PA and SN38 were 95.89% and 97.03%. The average absolutely recovery of SN38-PA and SN38 were 99.54% and 99.84%. The RSD for intra-day and inter-day were both less than 3%. Conclusion The method is fast, convenient, accurate and sensitive, so it can be used for determination of SN38-PA and SN38 in vivo.

Objective:To investigate the remodeling of alveolar bone and the changes of roots after an-terior retraction with maximum anchorage by analyzing CBCT data from adult cases.Methods:The sam-ple comprised 48 incisors and 24 canines from 12 patients (18 to 40 years of age)with problems of ma-xillary protrusion or upper arch protrusion.CBCT scans were exposed before and after treatment,and la-teral cephalometric images as well as multiple planar reconstruction images were reconstructed.Tracing superimpositions of sagittal sections and three-dimensional reconstructions were done for qualitative analy-sis.For all maxillary anterior teeth,changes of root length,alveolar bone height and labial-palatal thick-nesses at different levels were evaluated.The average of measurements taken by the same tester in three times was processed by SPSS 17.0 statistical package.Results:In 6 of the 12 cases,alveolar thickness became thinner on labial side [apical area:(-0.64 ±1.18)mm]while thicker on palatal side [apical area:(0.93 ±2.0)mm]and the root length decreased[(-0.95 ±0.79)mm].In the other 6 cases, the incisors’alveolar bone became thicker on labial side [apical area:(2.12 ±1.46)mm]while thin-ner on palatal side [apical area:(-2.88 ±0.58)mm]and the loss of root length was obvious[(-2.12 ± 1.43)mm].In all the 12 cases,the canines’alveolar bone became thinner on labial side especially on the apical level [(-0.27 ±1.86)mm]while greatly thicker on palatal side [apical area:(6.40 ± 6.00)mm]and the root resorption was slight [(-1.12 ±1.19)mm].For all the anterior teeth,the height of alveolar bone reduced around them after retraction.Conclusion:When the root apical moved more palatally,more root resorption would occur and the alveolar bone would get thicker on labial side but thinner on palatal side and thinner as a whole after anterior retraction with maximum anchorage.In the vertical direction,the height of the alveolar bone generally decreased on all sides and decreased the most on the palatal side.

Objective To investigate the effect of butylphthalide (NBP) on H2S content and the expression of NR2B in the hippocampus of alcohol dependence rats. Methods A total of 84 SD male rats were randomly divided into 6 groups. Except for the normal group, other groups were subjected to alcohol solution with concentration of 6% ( V/V) for 28 d. Drug intervention began at the 14th day,and rats in the low,medium,high dose group were treated with NBP with a different concentration. Erden abstinence scoring was used to evaluate the rats withdrawal symptom. H2S content was measured in one side of hippocampus and CBS activity was tested in the other side of hippocampus. Hippocampus of 3 rats from each group was used to investigate NR2B mRNA level. Results Withdrawal symptom score ( 12.27 ± 1. 19),H2S content(30. 25 ±8.82), CBS activity (72. 44 ±7. 46) and NR2B mRNA expression( 19. 47 ±0. 86) in medium dose NBP group rats were lower than withdrawal symptom score(14.09 ±2.21) ,H2S content(44. 50 ±6. 65) , CBS activity(79. 06 ±4. 57) and NR2B mRNA expression (29. 13 ±1.39) in experimental control group (P<0.05). Withdrawal symptom score(12. 18 ±1.08) ,H2S content(33.00 ±5.38) ,CBS activity(67. 81 ±9. 37) and NR2B mRNA expression(23. 12 ± 1. 86) in high dose NBP group rats were lower than experimental control group (P < 0. 05). Conclusion NBP can reduce withdrawal symptoms of alcohol dependence rats,may be related to decreased expression of H2S/CBS system, and NR2B mRNA expression.

Objective To explore the diagnostic value of prostate specific antigen (PSA),compound prostate specific antigen(cPSA),and cPSA / tPSA ratio in prostatic cancer(PCa).Methods Two hundred nineteen people,aged 62-68,were divided into three groups according to the clinical diagnosis,including PCa group(n=73),benign prostatic hyperplasia(BPH) group(n=76) and control group(n=70) composed of healthy people.The various indicators,including tPSA,cPSA and cPSA/tPSA were measured by Advia Centaur CP chemiluminescent analyzer.Results tPSA,cPSA and c/t significantly increased in PCa group compared with those in BPH or control group (P<0.05).ROC analysis indicated that if the tPSA cut-off values was19.89 μg/L,the sensitivity,specificity,the area under the curve (AUC) and Youden index would be 94.5%,97.9%,0.96 and 0.924 respectively.If the cut-off value of cPSA was 13.14 μg/L,the sensitivity,specificity,the area under the curve(AUC) and Youden index would be 95.9%,91.8%,0.98 and 0.877 respectively.The chi-square test showed that there is no correlation between tPSA,cPSA and pathology diagnosis(χ2=102.036、151.409,P<0.05).Conclusion PCa or PCa treatment relapse should be highly suspected when the concentration of tPSA or cPSA in patients reached 19.89 μg/L and 13.14 μg/L,the patients should be reminded for treatment timely.

OBJECTIVETo evaluate the difference of mesiodistal root angulation between panoramic radiograph and panoramic images reconstructed from cone beam computed tomography (CBCT) by different methods.

METHODSCBCT and panoramic radiograph of twenty patients were collected. The InvivoDental 5.0 was separately applied for maxillary or mandibular panoramic image reconstruction. The reconstruction method was combined by two head positions, the Frankfort plane horizontal position (P1) and the occlusal plane horizontal position (P2), and three central planes of focal trough (root apical plane, tooth center plane and crown marginal plane referring to the central incisor). Variation of tooth morphology in reconstructed panoramic images and panoramic radiograph was firstly observed. And then measurement for maxillary or mandibular anterior tooth intersection angle (contiguous angles among 321 123 were named as UA1-UA5; and those among 321 123 were named as LA1-LA5 ) was taken. The difference of intersection angles between reconstructed panoramic images and panoramic radiograph (ΔUA and ΔLA) were calculated. Wilcoxon Rank Sum Test was finally applied to compare the intersection angles' differences between P1 and P2 with the same central plane of focal trough.

RESULTSPanoramic images reconstructed from CBCT by tooth center plane with P1 for maxilla and P2 for mandible appeared to fulfill the clinically diagnostic demand through the observation. Among the 15 couples of comparison of intersection angles' differences between P1 and P2 by choosing the same central plane of focal trough for maxilla, 7 couples of ΔUA revealed a statistically smaller value in P1 while 1 couple of ΔUA showed an adverse result.In mandible with the same comparison method, 10 couples of comparison did not show statistical difference between P1 and P2, while 4 couples of ΔLA revealed a statistically smaller value in P2 and 1 couple ΔLA showed an opposite result.

CONCLUSIONSBy choosing the tooth center plane, the panoramic images reconstructed from CBCT with the Frankfort plane horizontal position in maxilla and the occlusal plane horizontal position in mandible were equally effective. The reconstructed panoramic images can be used as the panoramic radiograph in the assessment of the mesiodistal root angulation.

Cone-Beam Computed Tomography ; Dental Occlusion ; Humans ; Image Processing, Computer-Assisted ; Mandible ; Maxilla ; Radiography, Panoramic ; Tooth ; Tooth Crown ; Tooth Root ; diagnostic imaging

Objective To investigate the timing of super-selective renal artery embolization (SRAE) for the treatment of renal hemorrhage after percutaneous nephrolithotomy (PCNL) .Methods From June 2005 to February 2013 ,a total of 2 165 patients with upper urinary tract calculi underwent PCNL (2 384 PCNL procedures) and 16 of them suffered severe bleeding (0 .74% ) .In the 16 cases ,SRAE was used .The medical records of all the 16 cases were retrospectively analyzed .Results In 16 patients ,15 patients were successful with the first SRAE ,but 2 of them underwent an additional pure renal artery angiography (1 patient before SRAE and 1 patient after SRAE);1 healed after the second SRAE .The mean blood loss and transfusion volume were 32 .9 g/L and 250 mL before the first angiography/SRAE ,and an additional 3 .2 g/L and 0 mL before the second try .Although 1 patient died ,the oth-ers were recovered without complications .Conclusion SRAE should be adopted early for the treatment of severe renal hemorrhage after PCNL .However ,a second try should be considered for the repeated bleeding patients after the negative results of first renal artery angiography or SRAE .

OBJECTIVE: To investigate the effects of lead exposure and high fat diet on the hippocampal inflammatory factors and learning-memory in rats. METHODS: A total of 40 specific pathogen free male SD rats were randomly divided into control group,high fat diet group,lead exposure group,combine exposure group,10 rats in each group. Rats in control group were given regular diet and rats in high-fat diet group were given high-fat diet. Rats in lead exposure group were given regular diet and water with 400 mg/L lead acetate. Rats in combine exposure group were given high fat diet and water with 400 mg/L lead acetate. Body weight was measured every other week. The exposure period was 9 weeks. Morris water maze was applied to measure the learning-memory. The content of total cholesterol,triglyceride(TG),low density lipoprotein cholesterol(LDL) and high density lipoprotein cholesterol(HDL) in serum were detected by using microplate reader. The lead content of hippocampus was detected by inductively coupled plasma mass spectrometer. Enzyme-linked immuno sorbent assay was used to detect the content of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-4,IL-6 and interferon-γ(IFN-γ). RESULTS: Beginning from the third week,the body weight of rats in high fat diet and combine exposure group were significantly higher than that in control group(P < 0. 05). In addition,the body weight of rats in combine exposure group were higher than that in lead exposure group(P < 0. 05). Escape latency and the frequency of crossing platform of rats in high fat diet group,lead exposure group and combine exposure group were significant changed compared with those in control group(P < 0. 05). The escape latency of rats in combine exposure group increased compared with those in high fat diet group and lead exposure group(P < 0. 05). In addition,serum TG and LDL content in high-fat diet group and combine exposure group increased and HDL decreased compared with the control exposure group and lead exposure group(P < 0. 05). Compared with the control group and high fat diet group,the content of lead in hippocampus of lead exposure group and combine exposure group substantially increased(P < 0. 05). The levels of TNF-γ,IL-6,IL-1β,IFN-γ of hippocampus in high fat diet group,lead exposure group and combine exposure group were significantly higher than those in control group(P < 0. 05). Besides,the levels of IL-4 of hippocampus in lead exposure group and combine exposure group were higher than that in control group(P < 0. 05). IL-1β content of rats hippocampus in combine exposure group was higher than that in high-fat diet group or lead exposure group(P < 0. 05). CONCLUSION: Combine lead and high-fat diet exposure can exert a synergy in decrease of learning-memory in rats. IL-1β might involved in the process of synergic neurotoxicity induced by lead and high fat diet.