1.Simultaneous Determination of Imperatorin and Isoimperatorin in Yuanhu Zhitong Tablet by Capillary Electrophoresis
Hongquan GONG ; Chenghui ZENG ; Fuhai WU
China Pharmacy 2005;0(15):-
OBJECTIVE:To establish capillary electrophoresis method for the determination of imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS:The separation was performed on a uncoated fused silica capillary (55 cm?75 ?m ID,effective length of 47 cm) with 20 mmol?L-1 NaH2PO4+100 mmol?L-1 SDS+80%formamide as buffer solution (pH=7.12). The separation voltage was 15 kV and the sample was injected by gravity (5 s,15 cm). The detection wavelength was 254 nm. RESULTS:The linear range of imperatorin and isoimperatorin were 8.0～40.0 ?g?mL-1(r=0.998 7) and 4.0～20.0 ?g?mL-1(r=0.999 0). The average recovery was 101.3% for imperatorin (RSD=3.24%,n=6) and 100.2% for isoimperatorin(RSD=1.44%,n=6). CONCLUSION:The method is convenient,rapid and accurate for the quality control of Yuanhu zhitong tablet.
2.Pharmacokinetics of Valsartan and Hydrochlorothiazide in Healthy Volunteers after Single Administration of Its Compound Dispersible Tablets
Luqin SI ; Jiangeng HUANG ; Fuhai GONG ; Gao LI
China Pharmacy 2007;0(29):-
OBJECTIVE:To study the pharmacokinetics of hydrochlorothiazide and valsartan in healthy volunteers after single administration of its compound dispersible tablets.METHODS:Plasma concentrations of hydrochlorothiazide and valsartan were determined by HPLC and their pharmacokinetic parameters were calculated with DAS software.RESULTS:The pharmacokinetic parameters of hydrochlorothiazide at low,medium and high doses were as follows:t1/2 were(12.56?3.66),(11.47?5.47) and(11.20?5.03) h,respectively;Cmax were(72.00?19.68),(169.96?52.17) and(203.66?61.41)ng ?mL-1,respectively;AUC0～48 were(592.87?179.44),(1 155.45?252.03) and(1 410.99?331.82) ng?h?mL-1,respectively;AUC0～∞ were(779.76?201.42),(1 246.89?307.03) and(1 482.14?332.20) ng?h?mL-1,respectively.The pharmacokinetic parameters of valsartan at low,medium and high doses were as follows:t1/2 were(7.29?3.10),(8.56?2.22) and(8.62?2.86)h,respectively;Cmax were(2.12?0.65),(6.76?2.08) and(6.71?2.59)ng ?mL-1,respectively;AUC0～48 were(18.16?4.52),(41.77?10.86) and(51.77?27.45) ng?h?mL-1,respectively;AUC0～∞ were(19.07?5.56),(42.60?11.18) and(53.49?26.93) ng?h?mL-1 respectively.CONCLUSION:The pharmacokinetics for compound valsartan dispersible tablet fitted linear kinetics process.There is no interaction in pharmacokinetics between hydrochlorothiazide and valsartan when they used in combination.
Result Analysis
Print
Save
E-mail