1.Concise discussion on treating chronic prostatitis with treatment theory of pyocutaneous disease
Guozheng QIN ; Fugang ZHANG ; Baofu DONG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(12):-
Chronic prostatitis has similarity with pyocutaneous disease in pathogenesis oftraditional Chinese medicine and western medicine, pathology ofWestern medicine and signs and symptoms ofdisease, we got quiet cutative effect in treating chronic prostatitis from treatment theory ofpyocutaneous Disease.Based on theory ofdiagnosis and treatment ofpyocutaneous disease, we should synthesize differential diagnosis and treatment according to different stages, conform three methods, focus on eliminating method and strengthening vital qi, pay attention to two-level multi-object, involving macro-level and microlevel, and combination oftraditional and western medicine.The common used ancient prescriptions are Wuwei Xiaodu Yin, Gouju Decoction, Chaihu Shengshi Decoction.The common used empitical formula are Qinshi Simiao San, Qianliexian Xiaodu Fang, Qianlixian Tuodu Fang, Qianliexian Fuzheng Fang, Tongjing Huoxue Tang, except for these formula herbs, there are other common used herbs, such as processed zaoci, baijiangcao, hongteng, lianqiao etc.According to sydrome differentiation at stages ofbeginning, middle, the later, the chronic prostatitis can be divided into acute episode and chronic episode.In clinics, we should pay attention to taking care ofpatient's splee and stomach, increasing compliance ofpatient, inducing details ofdiet and living which will enhance clinical effective .
2.Interpretation of manuscript specification and common problems in writing
Yuan WANG ; Xianhua GUO ; Jinghui LIAO ; Lin ZHANG ; Haiying TANG ; Qing ZHANG ; Fugang LI
Chinese Journal of Radiological Medicine and Protection 2016;36(12):951-954
Writing and publishing scientific papers is a very important part of the research work.The quality of the thesis is also an important indicator to measure the achievements of scientific research workers.The quality of the thesis mainly includes the academic quality and the writing quality,but some of the researchers are mostly proficient in practice and neglect the writing,so there are still many writing problems in the manuscript.By reading the submission specification,this article summarizes the common problems in the writing,so as to provide the reference value for the readers.
3. Analysis of inter-laboratory comparison results of arsenic in urine and explore the influence factors on detection
Yajuan PAN ; Fugang ZHANG ; Jing ZHANG ; Huifang YAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(4):312-314
Objective:
Through inter-laboratory comparison to analysis and evaluation of the detection capacity of arsenic in the urine.
Methods:
Urine arsenic samples were prepared with normal human urine as matrix. The homogeneity of the samples was investigated and the results were statistically analyzed by single factor analysis of variance. 2 samples were issued to each participant laboratory. A robust statistical four-point distance method was used to calculate the results submitted by each participant laboratory and the test capability of the laboratory was assessed by the z-score method. By means of method experiments and records, the reasons of dissatisfaction and the influencing factors of the results were discussed.
Results:
The statistic of the homogeneity of urine arsenic samples was less than the critical value (
4.Effect of Thbs4 gene editing BMSCs transplantation on VEGF and Ang-1 in diabetic hind limb ischemia rats and related mechanism
Xiangfeng WU ; Tao WANG ; Tao LIU ; Fugang XU ; Qian ZHANG
International Journal of Biomedical Engineering 2019;42(4):294-300,316
Objective To investigate the effects and the mechanism of thrombospondin 4 (Thbs4) gene-edited bone marrow mesenchymal stem cells (BMSCs) transplantation on vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) in diabetic rats with hind limb ischemia. Methods Thirty Sprague-Dawley rats were randomly divided into the model group, BMSCs treatment group and Thbs4-BMSCs treatment group on average. After constructing the typeⅡdiabetic rat model with hind limb ischemia, 100μl normal saline, BMSCs suspension and Thbs4-BMSCs suspension (cell number: 2×106) were locally injected into the ischemic injury area of rats for the model group, BMSCs group and Thbs4-BMSCs group, respectively. The rats were sacrificed on the 14th day after stem cell transplantation, and the muscle tissues near the ischemic area were collected. The relative expression of VEGF and p-Smad2/3 protein was detected by Western Blot. The Ang-1 protein expression was detected by immunofluorescence staining. The levels of related genes were detected by qRT-PCR, and the von Willebrand Factor (vWF) protein expression was detected by immunohistochemistry staining. Results The relative expression levels of VEGF, Ang-1 and vWF protein in the Thbs4-BMSCs group were significantly higher than those in the model group and BMSCs group (VEGF protein:P<0.01 and P<0.05). The mRNA expression of VEGF and Ang-1 were significantly up-regulated, the differences were statistically significant(VEGF mRNA:all P<0.01;Ang-1:P<0.01 and P<0.05). The expression of p-Smad2/3 protein in the Thbs4-BMSCs group was significantly higher than that in the model group and the BMSCs treatment group (all P<0.01). The expression of p-Smad2/3 protein was significantly decreased after the addition of p-Smad2/3 pathway inhibitor, the differences were statistically significant (P<0.05). Conclusions Thbs4-BMSCs transplantation can effectively promote angiogenesis in diabetic rats with hind limb ischemia, and the effect of angiogenesis may be related to the activation of Smad2/3 signaling pathway.
5. Determination of fluorine-ion in human urine by ion chromatography method
Fugang ZHANG ; Huifang YAN ; Yajuan PAN ; Wei ZHAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(8):622-624
Objective:
To establish a method to determine fluorine ion in human urine by ion chromatography method.
Methods:
The samples were centrifuged, diluted and processed by C18 solid-phase column, potassium hydroxide as mobile phase, gradient elution, separated by AS15, and determined by anion conductivity detector.
Results:
The linear correlation coefficient of fluoride ion in the range of 0.02-10.00 mg/L was more than 0.999. The lowest detection limit of urinary fluoride was 0.06 mg/L (in 3 ml urine) . The recoveries of the method were 96.9%-99.5%, the inter batch precision range was 0.40%-2.89%. The intra batch precision was from 1.01 to 7.23%. Fluoride samples could be stored for 7 days at room temperature and 4 ℃, and be stored for 14 days at -20 ℃.
Conclusion
Determination of fluoride in urine by ion chromatography, the method could meet the requirement in GBZ/T 210.5-2008.
6. Optimization of ion selective electrode method in detection of urinary fluorine
Fugang ZHANG ; Huifang YAN ; Wei ZHAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(6):466-468
Objective:
To optimize the ion selective electrode method of measuring urinary fluorine in WS/T 30-1996.
Methods:
The volume of 5 mol/L NaOH solution was fixed to confect the TISAB buffer solution. The samples were prepared by mixing 5 ml urine and equal volume of TISAB solution. Fluorine electrode and calomel electrode was used to test. When the potential value changed less than ±0.5 mV in one minute, it could be recorded. The standard curve method was selected as a quantitive method.
Results:
The linear range of this method was 0.07~50.0 mg/L, E=238.96-57.50lgC,
7.Impact of Anticoagulation on Deep Vein Thrombosis Occurrence in Patients After Right Heart Catheterization
Xiaohui KUANG ; Xi ZHANG ; Xiaolong GAO ; Lilin WANG ; Liqun DING ; Jin ZHANG ; Hong XIANG ; Yating PENG ; Tian GAO ; Fugang MAO ; Jie FAN
Chinese Circulation Journal 2017;32(8):776-779
Objective: To explore deep vein thrombosis (DVT) occurrence in patients after right heart catheterization and the effect of anticoagulant therapy. Methods: A total of 171 consecutive patients with electrophysiological study (EPS) and/or radiofrequency catheter ablation (RFCA) in our hospital from 2015-01 to 2016-05 were enrolled. All patients had supra-ventricular tachycardia and completed a venous surgery, they were randomly divided into 2 groups: Anticoagulation group,n=87 and Non-anticoagulation group,n=84. Lower extremity vascular Doppler ultrasonography was performed at (24-48) h post-operation to compare the incidence of DVT between 2 groups. Results: There were 13/171 patients were excluded for not completing post-operative lower extremity vascular Doppler ultrasonography including 9 patients in Anticoagulation group and 4 in Non-anticoagulation group. 158 patients finished post-operative examination and follow-up study. Anticoagulation group had 7/78 (8.97%) patients suffered from DVT, Non-anticoagulation group had 41/80 (51.3%) patients suffered from DVT,P<0.001. Conclusion: The incidence of DVT was higher after right heart catheterization without anticoagulation; heparin treatment may reduce DVT occurrence in relevant patients.
8. Analysis on occupational health laboratories' proficiency testing program for determination of bovine blood lead and urinary cadmium
Yajuan PAN ; Xue TAO ; Wei ZHAO ; Jing ZHANG ; Fugang ZHANG ; Huifang YAN
Chinese Journal of Preventive Medicine 2018;52(2):180-184
Objective:
To analyze the results obtained from the proficiency testing program for determination of bovine blood lead and urinary cadmium, so as to evaluate the proficiency of the laboratories, and improve the detection and quality control capability of occapational health laboratory.
Methods:
Prepared 3 levels of lead samples with bovine blood as base material, taking Technical Norm of Primary Reference Material as Operating standard, 60, 80, 180 μg/L were used as the standard concentration. All samples were made for 200 bottles. Normal urine were used to prepared 3 levels of cadmium samples with 5.0, 7.0, 10.0 μg/L as standard concentration, then 3 level of samples were made for 200 bottles respectively. Blood lead and urinary cadmium samples were sent to each laboratory in the form of sample pairs. One-way ANOVA was used to investigate the homogeneity. The results of each laboratory were statistically analyzed by robust statistical method, and the detection ability of the laboratory was evaluated by the
9.Characteristics of electroencephalograph in cerebral infarction patients with subsequent vascular dementia
Fugang LUO ; Zhihua SHEN ; Yueqi HUANG ; Xiaoying ZHANG ; Meifang CHEN
Chinese Journal of General Practitioners 2018;17(2):136-139
Clinical data of 65 patients with acute cerebral infarction (frontal or temporal or both lobes) treated in our hospital during 2015-2016 were retrospectively reviewed.The patients were followed up for 6 months,34 cases were diagnosed as vascular dementia (VD) according to ICD-10 (VD group) and 31 cases were diagnosed as simple cerebral infarction (control group).The abnormal rate of electroencephalograph (EEG) at baseline in VD group was significantly higher than that in control group (x2 =5.44,P =0.02).Compared to control group,the powers of slow wave (θ wave and δ wave) at baseline,third month and sixth month in VD group were significantly increased (Fbet groups =17.39,Finner groups =163.50,Finteraction =38.71;all P =0.00) and (Fbetween groups =5.10,Finner groups =119.04,Finteraction =6.54;P < 0.05).Compared to baseline,the α wave and β wave were significantly decreased at third month and sixth month in VD (Finner groups =116.40,P =0.00) and (Finner groups =55.90,P < 0.05).Compared to control group,the powers of θ wave (except in the frontal lobe at baseline and third month) and δ wave were significantly increased at all time points (P < 0.05).Except the θ wave in occipital lobe,there were significant differences in slow wave at all time points between VD group and control group (P < 0.05).The featured EEG with increased slow wave in many regions of cerebral infarction can be used for early diagnosis of VD in patients with cerebral infarction.
10.Group rehabilitation training can improve the speech and life quality of persons with post-stroke dysarthria
Lijing CHANG ; Yahui WANG ; Jun XING ; Lizhuang ZHANG ; Fugang ZHAO
Chinese Journal of Physical Medicine and Rehabilitation 2020;42(9):792-796
Objective:To observe the effect of comprehensive rehabilitation training in a group on the speech and life quality of stroke survivors with dysarthria.Methods:Stroke survivors with dysarthria were randomly divided into an observation group (of 43) and a control group (of 41). In addition to routine medication, the control group was given 30 minutes of one-to-one rehabilitation training twice a day, 6 days a week for 4 weeks while the observation group was provided with 30 minutes of group training. Before and after the intervention, both groups were evaluated using the comprehensive dysarthria assessment scale and the WHOQOL-100 life quality assessment.Results:After the treatment, both groups′ average scores on the comprehensive assessment scale had improved significantly. Except for jaw positioning, all of the observation group′s other scores were significantly better than those of the control group, on average. The observation group also performed significantly better on the WHOQOL-100 assessment, on average.Conclusions:Group rehabilitation training can improve the speech function and life quality of stroke survivors with dysarthria significantly better than one-to-one training.