1.Study on realgar nanoparticles inhibition of adenovirus replication at the gene level.
Ming-Zhe WANG ; Wushouer FUERHATI ; Cheng-Xiang WANG ; Wen-Bo XU
Chinese Journal of Experimental and Clinical Virology 2013;27(5):357-359
OBJECTIVEModeling HAdV-3 infect Hep-2 cells in vitro. The effect of realgar nanoparticles on the expression of HAdV-3 is detected by using fluorescent quantitative PCR.
METHODSThe experiment is divided into four groups: Hep-2 cells control group, HAdV-3 virus control group, realgar nanoparticle group and ribavirin group. In order to detect HAdV-3 viral load, add realgar nanoparticles and ribavirin in vitro and remain that vitro for 24 hours when HAdV-3 has infected Hep-2 cells, extract total DNA of Hep-2 cells infected by HAdV-3, and establish Real-time PCR reaction system of every experimental groups.
RESULTThe Hep-2 cells group has no amplification curve, the Ct value is greater than 35, which illustrate HAdV-3 pathogen detection is negative. However, realgar nanoparticles group, ribavirin group and the HAdV-3 group have amplification curve, the Ct values are 29.30 +/- 0.08, 33.05 +/- 1.29, 26.01 +/- 0.25 respectively, which illustrate HAdV-3 pathogen detection is positive. The viral copy amount of the adenovirus group(66 699 932 +/- 23.85) is more than that of realgar nanoparticles group (912 435.44 +/- 16.57), and much greater than that of ribavirin group (459 124.84 +/- 12.82) (P < 0.05).
CONCLUSIONThe model of Hep-2 cell infected by HAdV-3 is reliable. The method of quantitative PCR is sensitive and specific. Realgar nanoparticles have a certain inhibition role for adenovirus nucleic acid replication.
Adenoviridae Infections ; virology ; Adenoviruses, Human ; drug effects ; genetics ; physiology ; Arsenicals ; chemistry ; pharmacology ; Gene Expression Regulation, Viral ; drug effects ; Hep G2 Cells ; Humans ; Nanoparticles ; chemistry ; Sulfides ; chemistry ; pharmacology ; Virus Replication ; drug effects
2. Analysis on epidemiological characteristics and related factors of dyslipidemia among adult residents of Xinjiang Uygur Autonomous Region during 2013-2014
Rong ZHANG ; Yunjun ZHANG ; Wushouer FUERHATI ; Peihua LIAO ; Wei ZHE ; Laixin LIU ; Mingjian NI
Chinese Journal of Preventive Medicine 2018;52(11):1152-1157
Objective:
To study the epidemiological characteristics and related factors of dyslipidemia among adult residents in Xinjiang Uygur Autonomous Region (Xinjiang) in 2013-2014.
Methods:
A total of 4 120 adult residents consisting of Han and Uygur group aged over 18 years old were selected by using a stratified cluster random sampling method in 8 counties of Xinjiang from 2013 to 2014. The related factors of dyslipidemia were collected by questionnaire and physical measurement. The total cholesterol, triglyceride, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were detected by enzyme method. Factors associated with dyslipidemia were analyzed by chi-squared test and a multivariate unconditioned logistic regression model adjusted for gender, urban or rural area, age-group, body mass index (BMI), central obesity, smoking, drinking, education attainment, diabetes mellitus and hypertension.
Results:
The prevalence of dyslipidemia was 45.00% (1 854 cases). The prevalence of dyslipidemia was higher in Uygur group (47.80% (977/2 044)) than that in Han group (42.24% (877/2 076)) (χ2=12.84,
3.An Investigation of the First Case of Human Rabies Caused by a Fox in China in May 2016.
Aierken TAXITIEMUER ; Gulina TUERDI ; Yi ZHANG ; Fuerhati WUSHOUER ; Xiao Yan TAO ; Jiangatai TALIPU ; Tuohetiyaer YIMAMU ; Hui Lai MA
Biomedical and Environmental Sciences 2017;30(11):825-828
This study assesses the causes and prevention measures of rabies through epidemiological investigation and analysis. A field epidemiological survey was conducted to investigate a case of rabies by fox bite. The onset of symptoms began 50 days after the bite. The patient did not receive standard treatment, rabies vaccination, or rabies immunoglobulin injection. The fox was killed on the spot. Saliva and pre-death blood samples were collected at different periods, and only blood RT-PCR tests yielded positive results. Wild fox bite is a major risk factor of rabies infection in Xinjiang Province, China.
Adult
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Animals
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Brain
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virology
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China
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epidemiology
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Fatal Outcome
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Foxes
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virology
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Humans
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Male
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Rabies
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epidemiology
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transmission
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Zoonoses