Objective To observe the effect of rehabilitation nursing on activities of daily living (ADL) of patients with stroke in Midway-training Course. Methods 51 stroke patients were divided into control group (n=26) and supervision group (n=25). The control group received routine rehabilitation service, and the supervision group received rehabilitation nursing supervisor in addition. They were assessed with Barthel Index (BI) before and after the course. Results The BI increased after course in both groups (P<0.05), and increased in the supervision group (P<0.05) after 30 d, but not in the control group (P>0.05). Conclusion Rehabilitation nursing during Midway- training Course can improve recovery of the ADL of patients with stroke.