OBJECTIVE:To study the effect of Herba Siegesbeckiae capsule on cerebral blood flow (CBF), cerebral vessel resistance (CVR), blood pressure (BP), electrocardiogram (ECG) and heart rate (HR)in anesthetized dogs. METHODS: The external carotid arteries on one side and the vertebral arteries of dogs were ligated, then the CBF of carotid arteries was measured by electromagnetic flowmeter, and the BP, EGG and HR were measured by polygraph. RESULTS: Herba Siegesbeckiae capusle could significantly increase CBF and decrease CVR; however, it has no effect on BP, ECG and HR. CONCLUSION: Herba Siegesbeckiae capsule can improve cerebral blood circulation through increasing CBF and decreasing CVR.

OBJECTIVE: To compare the protective effects of two fasudil hydroehloride injections (trade name: Yililu vs. Chuanwei) on experimental cerebral ischemia of rats. METHODS: A total of 60 SD rats were randomly divided into sham opertion group, model group, Yililu group, Chuanwei high dose group, and Chuanwei low dose group. Cerebral ischemia model was made in each group for the observation of the effects of fasudil hydroehloride injections on the neuralogical function, cerebral infarction size and the pathomorphology of brain tissues. RESULTS: As compared with model group, two kinds of the fasudil hydroehloride injections at the same dose identically decreased the score of neurological function as well as the cerebral infarction size, and lessened the degree of the pathomorphological change of brain tissues (P0.05). CONCLUSION: The two kinds of fasudil hydroehloride injections showed an equivalent protective effect on the damage of experimental cerebral ischemia.

AIM: To explore the curative effect of different ways of silicone tube catheter for the treatment of children`s lacrimal canaliculi rupture.METHODS: A retrospective analysis from January 2014 to December 2015 were taken.According to admission time sequence,42 children with lacrimal canaliculi rupture was divided into two groups.One group was treated with single silicone tube insertion,the other was treated with annular silicone tuber insertion.The treatment results were comparatively analyzed.RESULTS: There were 4 eyes in single silicone tube group occurred fall off in 2wk to 1mo after operations because of improper nursing and the second operations were taken.There was no eye with this in annular silicone tuber groups,the difference between the two groups was significant(P<0.05).In single silicone tube group,16 eyes were cured,1 eye improved,3 eyes unchanged which were the 3 eyes with fall off.Those 3 eyes were 1 patient bitten by dog,2 bombed;the effective rate was 85%.In annular silicone tuber insertion,20 eyes were cured,2 eyes improved and the effective rate was 100%,the difference between the two groups was significant (P<0.05).CONCLUSION: Children`s lacrimal canaliculi rupture should be timely given surgical treatment,reasonable surgical treatment can achieve good operation effect.Annular silicone tube has good stability,fewer complications than single silicone tube,and clinical treatment effect is good.

Objective To investigate the association of glyoxalase Ⅰ (GLO1) gene polymorphisms with susceptibility to atherosclerotic cerebral infarction (ACI).Methods Single nucleotide polymorphisms (SNPs) rs1130534 and rs1049346 in the GLO1 gene were genotyped in 300 ACI patients and 300 healthy controls using the SNaPshot technique.Additionally,quantitative real-time PCR was employed to determine the GLO1 expression levels in 50 ACI patients and 50 healthy controls,respectively.Results In terms of the rs1049346 polymorphism,the respective frequencies of CC,CT and TT genotypes were 9.3％,42.7％ and 48.0％ in the ACI patients,and 14.0％,47.7％ and 38.3％ in the control group.The respective frequencies of C and T alleles were 30.7％ and 69.3％ in the ACI patients,and 37.8％ and 62.2％ in the control group.There were statistically significant differences in the genotype and allele frequencies of rs1049346 between the ACI patients and controls (genotype:x2 =6.877,P =0.032;allele:x2 =6.842,P=0.009).For rs1130534,the respective frequencies of AA,AT and TT genotypes were 52.0％,42.0％ and 6.0％ in the ACI patients,and 60.0％,33.7％ and 6.3％ in the control group.The respective frequencies of A and T alleles were 73.0％ and 27.0％ in the ACI patients,and 76.8％ and 23.2％ in the control group.However,no statistically significant differences were found in the distribution of genotypes or alleles of the rs1130534 SNP between the ACI patients and controls (all P ＞ 0.05).The results of haplotype analysis revealed that the frequencies of the A-T and T-T (rs1130534-rs1049346) haplotypes in the ACI patients were all significantly higher compared to the controls (42.3％ vs 39.0％,x2 =4.733,P =0.030;27.0％ vs 23.2％,x2 =5.699,P =0.017).Additionally,the GLO1 expression levels in the ACI patients were significantly lower than that in the healthy controls (Mann-Whitney U =911.5,P =0.020).Conclusion The results indicate that the rs1049346 polymorphism of GLO1 gene is associated with the susceptibility to ACI.

Biopsy ; Carcinoma, Hepatocellular ; diagnosis ; Diagnostic Errors ; Fatal Outcome ; Humans ; Liver ; diagnostic imaging ; pathology ; Liver Neoplasms ; diagnosis ; Male ; Middle Aged ; Splenectomy ; Splenic Diseases ; diagnosis ; surgery ; Tomography, X-Ray Computed

It is now widely accepted that platelet aggregation plays an important role in physiological hemostasis and pathological thrombosis associated with cardiovascular and cerebrovascular diseases. Anti-platelet aggregation drug research is also a hot spot of current research. The biggest challenge of antiplatelet therapy has been the molecular overlap of the hemostasis and thrombosis, leading to a serious risk of bleeding. Recent studies have emphasized the importance of shear stress generated from blood flow, which will primarily drive platelet activation and aggregation in thrombosis. So if we can take advantage of the differences between the physiological and pathological vascular blood flow environment, the development of selective anti-platelet therapy may be a safer treatment for cardiovascular and cerebrovascular diseases. In this review, we discuss the underlying mechanisms of shear-induced platelet activation. Later, we summarize the effects and mechanisms of compounds and traditional Chinese medicine on shear-induced platelet activation. The aim is to provide a reference for the study of biological pharmacology of traditional Chinese medicine for promoting blood circulation and removing blood stasis.

Objective To investigate the expression of miR-200b in lung cancer and its relationship with clinicopathological features of lung cancer.Methods The specimens of lung tumor tissue and adjacent normal lung tissue of 36 cases of lung cancer who received surgical treatment in our department were collected,and then quantitative real time PCR (qRT-PCR) was applied to determine the expression of miR-200b in lung cancer tissue and adjacent normal lung tissue.Results The expression of miR-200b in lung cancer was significantly lower than adjacent normal lung tissue (P =0.000),and in small cell lung cancer was also significantly lower than adenocarcinoma and squamous cell carcinoma (0.13 ± 0.09 vs.0.64 ± 0.33,0.75 ± 0.30) (P =0.005 and P =0.001).The expression of miR-200b in patients with positive lymph nodes,advanced stage of lung cancer and in smokers were significantly lower than those with negative lymph nodes,early stage of lung cancer and in non-smokers,respectively (0.52 ± 0.29 vs.0.87 ± 0.35,0.46 ±0.25 vs.0.90 ±0.32,0.52 ±0.27 vs.0.90 ±0.39) (P =0.004,P =0.000 and P =0.015).Conclusions Low expression of miR-200b may participate in the occurrence and progression of lung cancer,especially in small cell lung cancer,and correlates with the metastasis of lung cancer.The down-regulated expression of miR-200b may be induced by smoking.Thus,miR-200b perhaps is a new target for the treatment of lung cancer.

Varicella zoster virus (VZV) is a widespread human herpesvirus which causes chickenpox (varicella). VZV infection can produce a broad spectrum of neurologic disorders including multiple cranial nerve palsies. Among the cranial nerves, trigeminal and facial nerves are the most commonly involved. However, multiple lower cranial nerve palsies caused by VZV infection is very rare. It is a diagnostic dilemma for VZV infection if there are no skin or mucosa lesions, which are characteristic signs of VZV infection. We report the case of a 54-year-old man with sudden right hearing loss, sore throat, odynophagia, hoarseness, dysphagia and vertigo. Pure tone audiometry revealed right sensorineural hearing loss and laryngoscopy proved that there were paralyses of the right upper pharyngeal constrictor muscle and vocal cord. These findings were consistent with acute unilateral vestibulocochlear, glossopharyngeal and vagus nerve palsies. There were no skin or mucosa lesions noted in our patient. The diagnosis was assisted by the existence of anti-VZV serum IgM even if the polymerase chain reaction result was negative. The symptoms of the patient improved after receiving anti-viral therapy. We emphasize the role of VZV infection in multiple cranial nerve palsies and the importance of serologic test.
Herpesvirus 3, Human ; Cranial Nerve Diseases

Objective To form the basis of establishing a strategy for safe blood donor recruitment and to identify and subdivide the structures of donors.Methods Demographic data of blood donors, and blood testing results of HBsAg, anti-HCV, anti-HIV, and syphilis, from Nov. 2004 to Jun. 2005, were collected and analyzed.Results Among the 45270 voluntary blood donors, the total positive test rate were 3.11%, which was related to age, level of education and occupation of blood donors. About 76.7% of donors were young people, aged from 18 to 30. As the age increased, female donors were less than male ones. Among all the donors, 81.6% received higher education, which had lower positive test rate than those with lower level of education. Among the 45270 donors, farmers had the highest positive test rate; workers and service people came the second; students and medical staff had the lowest. As to donors with incomplete record, the positive test rate was higher than those with complete record.Conclusion During blood donor recruitment, quality control is important. People younger than 30, with student or higher education background, are relatively safe to recruit.

OBJECTIVE To understand the nurses′ awareness of specimen collection and explore the impact of acquisition-related factors and provide the basis for standardized training.METHODS A questionnaire was designed for specimen collection survey about microorganisms in level-3 hospital.RESULTS 90.38 percent of nurses consulted the laboratory,80.77 percent communicated with other nurses to solve the problom about microbiology specimen collection and 94.2% thought it necessary about continuing education.The ward nurses′ knowledge about microbiology specimen collection was better than these from surgical department.CONCLUSIONS It is urgent that stundard training of specimen collection should be implement in medintely due to lacking the awareness.