[Objective]Modify Xuejiejiawei Granule judgment toxic particles to guide its clinical drug safety.[Method]Make different dosages,give the mouse to fill stomach,from the observing mouse's physiological condition to judging the medicine toxic.[Result]36 mice after given the medicine observation period do not have an example to die,after the dissection the internal organs have non-pathological changes.[Conclusion] Looking at Xuejiejiawei Granule from the medicine toxic effect,the Xuejie adding the taste pellet is a safe medicine.

Objective To explore the correlative factors of asthma after bronchiolitis.Methods Clinical data from 115 hospitalized patients with bronchiolitis were collected from Jan.2002 to May 2003,including age,gender,incidence season,family asthma history,birth weight,eczema,severity of bronchiolitis,pathogen,total and specific immunoglobulin E(IgE),overweight,smoking during pregnancy and feeding patterns;and the data of respiratory tract infection and wheezing onset in those cases were collected by clinic sevice and telephone follow-up in post-discharge for 5 years;the pollution status in living environment(coal dust and mine especially) and animal contact history(especially cats and dogs) were recorded.And the independent effect of risk factors was obtained,Logistic regression models were created for each outcome variable.Results Thirty-nine(33.9%) cases of 115 children with brochiolitis developed into asthma.There were significant differences between asthma and non-asthma group in family asthma history,recurrent lower respiratory tract infection(LRTI) under 1 year old,onset age,severity extent and obesity(P =0,0,0.004,0.004,0.020,respectively).The factors that contribute to the risk of asthma onset were incidence season,severe bronchiolitis,parental asthma,eczema and recurrent LRTI(OR=0.49,3.82,6.12,3.76 and 3.14,respectively P=0.000 7,0.026 0,0,0.030 9,0.000 5).But,the factors of gender,birth weight,congenital heartdisease,pollution status in living envi-ronment,animal contact history,feeding patterns,smoking during pregnancy,age when occured bronchioliyis,blood IgE and overweight had not correlated with asthma after bronchiolitis.Conclusions Severe bronchiolitis,parental asthma,eczema and recurrent LRTI under 1 year old correlate with development of asthma after bronchiolitis,however,falling ill in winter may be a protective factor in the progression of airway inflammation.

Objective To explore the factors affecting severity of infants with bronchiolitis in hospital.Methods Data were collected from hospitalized case of bronchiolitis including age,gender,birth weight,incidence season,overweight,severity of bronchiolitis,clinical manifestation,pathogen,total and specific immunoglobulin-E (IgE),X-ray appearance,family asthma history,eczema,feeding patterns,congenital heart disease,countryside dwelling and length of stay.All cases were divided according severity extent into 2 groups.Then one-factor analytical method was used between 2 groups,and risk factors for these outcomes were identified by Logistic regression analysis.Results 1.Nearly 56%(506 of 904) of children were considered as obesity.2.The positive rate of the virus infection identified from 904 infants with bronc-hiolitis was 78.3%.Of them,the respiratory syncytial virus (RSV) infection accounted for 43.5%,cytomegalovirus (CMV) for 47.6%,coxsackie b virus (CBV) for 7.9%,Mycoplasma pneumonia (MP) for 1.8%,adenovirus (ADV) for 1.7% and epstein-barr virus (EBV) for 0.15%,respectively.3.There were notable differences between mild group and severe group in age,pathogen,birth weight,countryside dwel-ling,congenital heart disease and hospital day(?2=3.47,19.62,2.32,2.61,-4.71,69.23 P=0.001,0.006,0.02,0,0,0).There was a significant association between severe bronchiolitis and young age,low birth weight,congenital heart disease,countryside dwelling and hospital days (OR=0.840,0.542,15.412,5.305 and 1.173,respectively P=0.013 3,0.049 7,0.000 3,0,0.000 2).Conclusions 1.Obesity may have great risk for bronchiolitis.2.Except for RSV,CMV,CBV,ADV,MP and EBV all were pathogens associated with childhood bronc-hiolitis.3.The factors that contribute to increase risk of severe bronchiolitis are young age,low birth weight,congenital heart disease and countryside dwelling.And length of stay may estimate severity of infants with bronchiolitis in hospital.

0.05) . CONCLUSION: Gatifloxacin has no significant influence on pharmacokinetics of the hepatic injury model group as compared with the normal group.

OBJECTIVE:To study the pharmacokinetics and relative bioavailability of two kinds of preparations of valaciclovir hydrochloride.METHODS:This was a randomized two-way cross-over study.A total of 18 healthy volunteers were randomly assigned to receive single oral dose of test valaciclovir tablets(trial group) or reference valaciclovir tablets(control group),with the plasma aciclovir concentrations determined by HPLC,the pharmacokinetics parameters calculated and the relative bioavailability evaluated using 3p97 program.RESULTS:The main pharmacokinetic parameters test and reference valaciclovir hydrochloride tablets were as follows:AUC0～ 24:(12.85? 4.32) vs.(12.19? 4.63) ?g? h? mL-1;AUC0～ ∞:(14.65? 5.75) vs.(13.27? 5.03) ?g? h? mL-1;Cmax:(3.55? 0.92) vs.(3.71? 0.97) ?g? mL-1;tmax:(1.44? 0.43) h vs.(1.33? 0.37) h;t1/2:(6.23? 2.81) h and(4.55? 1.84) h.The relative bioavailability of the test valaciclovir tablets were(111.01? 23.52) %.CONCLUSION:The reference valaciclovir tablets and the test valaciclovir tablets were bioequivalent.

Objective To evaluate the relationship between the cervical length,the test of fetal fibronectin and premature birth in pregnant woman.Methods Fetal fibronectin in sample of cervical secretion was tested using the reagent box of FFN manufactured by the ADEIA company of the United States and enzyme-labeled immunosorbent assay(ELISA) methods in 110 pregnant women.They were divided into two groups:first group,there were 60 cases who had symptom of aura prematurity and cervical lengths ≤30 mm monitored by trans-perineum ultrasound.second group was 50 normal pregnant women.Results The postive rate of fetal fibronectin was higher significantly in first group than that in second group.Conclusion The measurement of cervical lengths by the trans-perineum ultrasound and the test of Fetal fibronectin in pregnant women who has symptom of aura prematurity are of certain clinical value in predicting preterm birth.

Objective To evaluate the curative effect and problems of X-ray fluoroscopy guided percutaneous vertebroplasty (PVP) in treating osteoporotic spinal compression fractures. Methods Twenty-four patients (15 females and 8 males) with 44 vertebral compression fractures underwent PVP. They were average 69 years of age (48-83 years). The fracture segment was within T 5-L 3 (20 thoracical vertebrae, 24 lumbar vertebrae). Under the guidance of C-arm fluoroscopy, bone marrow biopsy needle was inserted percutaneously via transpedicular way into the fractured vertebrae. Polymethylmethacrylate (PMMA) was then injected into the fractured vertebrae. Vasual analogue scale (VAS), mobility and analgesic usage were evaluated 2 days before and after PVP and in the follow-up period. Results PVP was successful in 21 cases (40 vertebrae ), but failed in 2 cases (4 vertebrae) due to weak position endurance. The volume of PMMA injected was 1.0-8.0 ml per vertebrae. The average follow-up period was 3.1 months (1-7 months). VAS was sharply decreased from pre-PVP 7.5?1.2 to 2.8?1.0 at day 2 after PVP (P

Objective To approach the relationship between the membrane protein of platelet microparticles and myocardial infarction or hypertension. Methods The contents were examined of PAC-1~ + PMP and CD62P~+ PMP by activating platelet with 20?mol/L ADP in the blood collected from the patients with myocardial infarction (n=12) and hypertension (n=10). Results The contents of PAC-1~ + PMP and CD62P~+ PMP in myocardial infarction group (85.1%?3.7%, 85.9%?3.8%) were significantly higher than normal group (75.5%?4.4%, 76.3% 5.3%) (P

The immunoregulatory effects of some anti-cancer drugs have important role in their anti-tumor mechanisms. Our results showed that doxorubicin (DXR) of 0.04 mg ?L-1 could enhance the interleukin 2 (IL-2) release and induction of lymphokine-activated killer (LAK) activity of human fetal thymocytes(HFT). Higher concentration of this drug (4 mg ?L-1) exhibited inhibition on IL-2 release but no effect on LAK activity. These data suggest that lower concentration of DXR have some immunopotentiation effects on HFT.

Graphene/Room temperature ionic liquids ( GN/IL ) nanocomposite was prepared by grinding graphene and ionic liquid 1-butyl-3-methylimidazolium bromide ( BMIMPF6 ) which mixed together in appropriate proportion. Atomic force microscopy ( AFM ) was utilized to characterize the formation of the GN-ILs. Due to the synergistic effect between ionic liquids and graphene, the nanocomposite exhibited excellent performance toward H2 O2 reduction. A novel uric acid ( UA) electrochemical sensor was fabricated based on uricase-GN/IL modified glassy carbon electrode. The experimental results showed that the response displayed a good linear response toward UA in the concentration range from 0. 002-4. 5 mmol/L. The corre1ation coefficient was 0. 995 and the detection limit was 0. 85 μmol/L. The easily prepared electrochemical sensor had favorable stability and selectivity and could be applied to the quick determination of UA in human serum, thus providing a new UA detection method for clinical trial.