1.Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines
Yazan Saiful Latifah ; Rahman Abd Napsiah ; Chan Wei Kim ; Tor Sim Yin ; Foo Biau Jhi
Asian Pacific Journal of Tropical Biomedicine 2016;6(5):404-409
Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction.
Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer (MCF-7 and MDA-MB-231), colon cancer (HT29), lung cancer (A549), cervical cancer (HeLa) and normal mouse fibroblast (3T3) cell lines was deter-mined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope.
Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction (P <0.05). The IC50 of n-butanol fraction for HT29 and MCF-7 was (780.00 ± 28.28) and (895.00 ± 7.07) mg/mL, respectively. Cell shrinkage, membrane blebbing and for-mation of apoptotic bodies were noted following treatment of HT29 cells with n-butanol fraction.
Conclusions: In conclusion, n-butanol fraction was more cytotoxic than crude ethanolic extract and aqueous fraction towards the selected cancerous cell lines and induced apoptosis in HT29 cells.
2. Phenolics-saponins rich fraction of defatted kenaf seed meal exhibits cytotoxicity towards cancer cell lines
Latifah Saiful YAZAN ; Napsiah ABD RAHMAN ; Kim Wei CHAN ; Wan Nor Hafiza WAN ABD GHANI ; Yin Sim TOR ; Jhi Biau FOO ; Latifah Saiful YAZAN ; Napsiah ABD RAHMAN ; Wan Nor Hafiza WAN ABD GHANI ; Yin Sim TOR ; Jhi Biau FOO
Asian Pacific Journal of Tropical Biomedicine 2016;6(5):404-409
Objectives: To determine the cytotoxicity of crude ethanolic extract, n-butanol fraction and aqueous fraction on selected cancer cell lines, and to observe the morphological changes of the cancer cells treated with n-butanol fraction. Methods: The cytotoxic effect of n-butanol fraction, crude ethanolic extract and aqueous fraction on breast cancer (MCF-7 and MDA-MB-231), colon cancer (HT29), lung cancer (A549), cervical cancer (HeLa) and normal mouse fibroblast (3T3) cell lines was determined using MTT assay. The morphological changes of the treated cells were observed under an inverted light microscope. Results: n-Butanol fraction was the most cytotoxic towards HT29 and MCF-7 cells in a dose-dependent manner compared to crude ethanolic extract and aqueous fraction (P < 0.05). The IC
3. Anti-breast cancer properties and toxicity of Dillenia suffruticosa root aqueous extract in BALB/c mice
Latifah Saiful YAZAN ; Yong Sze ONG ; Jhi Biau FOO ; Yin Sim TOR ; Latifah Saiful YAZAN ; Nur Elena ZAABA ; Razana Mohd ALI
Asian Pacific Journal of Tropical Biomedicine 2015;5(12):1018-1026
Objective: To determine the anti-breast cancer activities and the safety oral consumption of Dillenia suffruticosa root aqueous extract (DRAE) in BALB/c mice. Methods: In the anti-breast cancer study, female BALB/c mice were divided into five groups (n = 12), which were (1) positive control (with breast cancer, untreated), (2) negative control (without breast cancer, untreated) and other three groups of mice with breast cancer treated with 1000, 500 and 250 mg/kg of DRAE, respectively, by oral gavage for 28 days. All mice except from the negative control group were injected into the mammary fat pad with 4T1 cells (1 × 10