No abstract available.
Flow Cytometry* ; Microscopy*

No abstract available.
Flow Cytometry* ; Microscopy*

No abstract available.
DNA* ; Flow Cytometry*

No abstract available.
DNA* ; Flow Cytometry*

No abstract available.
Adenocarcinoma* ; Flow Cytometry*

No abstract available.
Flow Cytometry* ; Microscopy* ; Urinalysis*

No abstract available.
Flow Cytometry* ; Mastocytosis, Systemic* ; Splenomegaly*

In order to meet the needs of the flow cytometry for the simultaneous analysis of multiple fluorescence wavelengths and small volume, the design method of flow cytometry spectrum analysis system is presented by analyzing the characteristics of Dyson structure. And according to the method, a flow cytometry spectrum analysis system is disigned with Dyson type.The system's spectral range is 400 nm to 800 nm, the defocused spot size is less than the pixel size 24μ mm, the ransfer function value is above 0.8 at the Nyquist cut-off frequency 21 lp/mm,the spectral resolution is less than 3 nm, and the overall size is 83.54 mm×85.60 mm.The system has good optical performance and small volume, which meets the needs of the flow cytometry fluorescence spectral analysis. The outstanding innovation of this system is the application of Dyson light splitting structure and EMCCD detector which is high speed and high sensitivity.
Equipment Design ; Flow Cytometry ; instrumentation

BACKGROUND: In flow cytometry, the verification of the anti-human immunoglobulin (anti-Ig) reactivity is important. The author has devised a simple protocol for this. METHODS: As a staining verification control, red blood cells (RBCs) were used. IgG- or IgM- coated RBCs were made using group O or group B serum, respectively. Ani-Ig reactivity in each serially diluted serum for immunoglobulin coating and correlation between the amount of reacting anti-Ig on RBCs and measured values were investigated. In one case of parallel tests for two anti-Ig reagents, author's method was compared with the B cell gating method. RESULTS: Both the test/control mean channel fluorescence ratio and percent fluorescence had significant linear correlations with the amount of the reacting anti-IgG on RBCs. As well as B cell gating, the author's method seemed to also clearly discriminate the reactivity of two anti-IgG reagents. CONCLUSIONS: Using immunoglobulin-coated RBCs as a staining verification control, the parallel test for two anti-IgG reagents could be performed more conveniently and objectively than the B cell gating method.
Erythrocytes ; Flow Cytometry* ; Fluorescence ; Immunoglobulins* ; Indicators and Reagents*

T cells are an essential cellular component of the immune system. When T cells encounter antigen and receive co-stimulatory signals, they become activated, proliferate and produce cytokines. Flow cytometry is a valuable research tool in analyzing T cell functions including proliferation, survival and cytotoxicity as well as cytokine production and cell signaling. This article has reviewed the utility of flow cytometry in evaluating T cell functions.
Cytokines ; Flow Cytometry ; Immune System ; T-Lymphocytes