1.Preparation and acute toxicology of nano-magnetic ferrofluid.
Zefeng, XIA ; Guobin, WANG ; Kaixiong, TAO ; Jianxing, LI ; Yuan, TIAN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(1):59-61
The nano-magnetic ferrofluid was prepared by chemical coprecipitation and its acute toxicology was investigated. The effective diameter (Eff. Diam. ) of the magnetic particles was about 19.9 nm, and the concentration of the ferrofluid was 17. 54 mg/ml. The acute toxic reaction and the main viscera pathological morphology of mice were evaluated after oral, intravenous and intraperitoneal administration of the nano-magnetic ferrofluid of different doses respectively. Half lethal dose (LD50) > 2104. 8 mg/kg,maximum non-effect dose (ED0) = 320. 10mg/kg with oral; LDs,> 438. 50 mg/kg, EDo = 160. 05 mg/kg with intravenous route; and LDso >1578. 6 mg/kg, ED0 = 320. 10 mg/kg with intraperitoneal administration. Degeneration and necrosis of viscera were not found. So the nano-magnetic ferrofluid, of which toxicity is very low, may be used as a drug carrier.
Ferrosoferric Oxide/chemical synthesis
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Ferrosoferric Oxide/*toxicity
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Magnetics
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Nanostructures/*toxicity
2.Combined Toxicity of an Environmental Remediation Residue, Magnetite Fe3O4 Nanoparticles/Cr(VI) Adduct.
Zhuan LI ; Miao LIU ; Li Ke CHEN ; Guang Zhu LI
Biomedical and Environmental Sciences 2017;30(11):783-791
OBJECTIVEThis paper aims to elucidate the combined toxicity of magnetite nanoparticles/Chromium [MNPs/Cr(VI)] adducts.
METHODSThe HEK293 cell was exposed to either Cr(VI) or MNPs, or their adducts MNPs/Cr(VI). The cytotoxicity was evaluated by assessing the cell viability, apoptosis, oxidative stress induction, and cellular uptake.
RESULTSThe toxicity of formed adducts is significantly reduced when compared to Cr(VI) anions. We found that the cellular uptake of MNPs/Cr(VI) adduct was rare, only few particles were endocytosed from the extracellular fluid and not accumulated in the cell nucleus. On the other hand, the Cr(VI) anions entered cells, generated oxidative stress, induced cell apoptosis, and caused cytotoxicity.
CONCLUSIONThe results showed minor effects of the nanoadducts on the tested cells and supported that magnetite nanoparticles could be implemented in the wastewater treatment process in which advantageous properties outweigh the risks.
Chromium ; chemistry ; toxicity ; Environmental Restoration and Remediation ; methods ; Ferrosoferric Oxide ; chemistry ; toxicity ; HEK293 Cells ; Humans ; Metal Nanoparticles ; chemistry ; toxicity
3.Security evaluation of subcutaneous injection with water-based dextran-coated magnetic fluid.
Yu ZHAI ; Xiaoliang WANG ; Xuman WANG ; Hong XIE ; Hongchen GU
Journal of Biomedical Engineering 2006;23(6):1275-1278
Water-based magnetic fluid was synthesized by using 50% dextran 40,000 as coated reagent. The acute toxicity and irritant of the magnetic fluid injected into mice subcutaneous tissues were examined. The lethal dosage 50 of dextran-coated magnetic fluid was 4409.61 +/- 514.93 mg/kg. Twenty four h after subcutaneous injecting with 30 mg/0.3 ml dextran-coated magnetic fluid, no more inflammation than hemangiectasia and leucocytes infiltration had been seen in subcutaneous tissues, 72 h later the reaction phenomena disappeared. While, injection with 30 mg/0.3 ml water-based oleate sodium-coated magnetic fluid, ulceration and break-off of cutis had been seen in the seventh days. That is to say, the dextran-coated magnetic fluid was safe and well tolerate, however, the oleate sodium-coated magnetic fluid was not fit to subcutaneous injection.
Animals
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Coated Materials, Biocompatible
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toxicity
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Dextrans
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toxicity
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Female
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Ferrosoferric Oxide
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toxicity
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Injections, Subcutaneous
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Magnetics
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Male
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Mice
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Mice, Inbred BALB C
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Random Allocation