1.Influences of the cognitive behavior therapy on anxiety for postoperative patients with oral and maxillofacial malignant cancer in surgery intensive care unit
Yang JIANG ; Mingou CUI ; Fengzhou CHEN
Chinese Journal of Practical Nursing 2017;33(23):1802-1805
Objective To investigate the effects of the cognitive behavior therapy on anxiety for postoperative patients with oral and maxillofacial malignant cancer in surgery intensive care unit. Methods A total of 82 patients with oral and maxillofacial malignant tumor who accept surgery from January 2014 to September 2016 were chosen and averagely divided into intervention group with cognitive behavior therapy based on control group and control group taking routine nursing and general psychological care by random number table in SICU. The application of self-evaluation of anxiety scale (SAS) evaluation of anxiety level before and after the intervention in patients with and without improvement, and compare the two groups of stay in SICU patients satisfaction evaluation intervention is effective. Results Compared with control group, the cognitive behavioral therapy, after intervention group patients with SAS scoring[(50.56 ± 6.52) ]points SAS scores than the control group [(57.51 ± 6.11)] points decrease, the difference was statistically significant (P<0. 01);intervention group check in SICU satisfaction (97.6%,40/41) also better than the control group (90.2%, 37/41), the difference was statistically significant (χ2=7.527, P < 0.05). Conclusion cognitive behavioral therapy can effectively reduce the anxiety of postoperative patients with oral and maxillofacial malignant cancer in SICU, and effectively improve the comfort level and satisfaction in SICU.
2.Construction of lentiviral vector carrying mitochondrial calcium uptake 1 and its use in infected H9C2 cells
Zhe JING ; Fengzhou LIU ; Yan LIU ; Yongqing CHEN
Chinese Journal of Tissue Engineering Research 2016;20(37):5560-5566
BACKGROUND:Mitochondrial calcium uptake 1 (MICU1) is one of the important molecules to maintain the mitochondrial calcium homeostasis. The regulation of MICU1 to mitochondrial calcium homeostasis may play an important role in diabetic cardiomyopathy, but the underlying mechanism remains unclear.
OBJECTIVE:To construct a lentiviral vector carrying MICU1 gene to transfect H9C2 cel s, and then to assess MICU1 level in H9C2 cel s thereby establishing a platform for researching the occurrence and development of diabetic cardiomyopathy at a cel ular level.
METHODS:DNA fragments of MICU1 were amplified by PCR, cleaved with Spe I, EcoR I and cloned into the lentiviral vector pRRLsin.CMV.eGFP to construct pRRLsin.CMV.MICU1-eGFP vector. 293T cel s were co-transfected with recombined pCMVDR8.91 and pCMV-VSVG to produce pRRLsin.CMV.MICU1-eGFP lentiviral viruses, and then used to infect H9C2 cel s. mRNA and protein expressions of MICU1 in the transfected H9C2 cel s were evaluated by real-time PCR and western blot assay. Mitochondrial calcium level in Rhod-2-stained H9C2 cel s was tested under confocal microscope.
RESULTS AND CONCLUSION:The recombinant inducible lentiviral vector containing MICU1 gene was successful y constructed. 293T could express green fluorescent protein with increased MICU1 level after pRRLsin.CMV.MICU1-eGFP transfection. The mRNA and protein expressions of MICU1 in the infected H9C2 group were obviously up-regulated compared with the other groups. MICU1 could remarkably improve the mitochondrial calcium level under Rhod-2 staining. These results show that pRRLsin.CMV.MICU1-eGFP lentiviral viruses are efficient to transfect H9C2 cel s, which wil be powered to lay a foundation for the immortalized cel line establishment.
3.The value of using B-type natriuretic peptide and D-dimer in preliminary recognition of cardioembolic stroke patients
Zhixin WU ; Yingying LI ; Sina LIANG ; Fengzhou QING ; Junna LEI ; Jingli CHEN ; Mingfeng HE ; Kuangyi LI
The Journal of Practical Medicine 2018;34(1):44-48
Objective To determine the value of using B-type natriuretic peptide (BNP) and D-dimer in preliminary recognition of cardioembolic stroke patients.Methods A mutilple-center study was conducted in Foshan Hospital of traditional Chinese Medicine (TCM) and its affiliated hospitals from July 2015 to July 2016.In the emergency departments (EDs),emergency physicians prospectively assessed consecutive adult patients with acute cardioembolic stroke and measured plasma BNP by POCT platform on admission,then followed up.Stroke neurologists evaluated patients' functional outcome at hospital discharge and also made discharge diagnosis and stroke etiologic subtypes according to the TOAST criteria.Results In this study,290 acute ischemic stroke patients met the study criteria [mean age (68.41 ± 12.06) years;53.8% female].Of the enrolled patients,28.3% were diagnosed with LAA at discharge,17.9% with CE,42.8% with SAO,11.0% with SOE or SUE.And the mean BNP concentration was significantly higher in the CE group than that in other three subtypes (P < 0.001).After adjustment for multiple clinical predictors like gender,age,coronary artery disease,atrial fibrillation and renal function,BNP and D-dimer were associated with CE [BNP OR:1.044 (95% CI 1.025,1.064),P < 0.001;D-dimer OR:1.511(95% CI 1.020,2.238),P =0.039,respectively].Conclusion Through POCT technique in the EDs,cardioembolic stroke patients can be differentiated from other TOAST subtypes.BNP with/without D-dimer has good but different corresponding diagnostic performance in preliminary recognition of cardioembolic stroke patients.
4. Volumetric measurement of alveolar bone defect
Jing LIU ; Yongqian WANG ; Fengzhou DU ; Shuxiu CHEN ; Binghang LI ; Haidong LI ; Tao SONG ; Di WU ; Ningbei YIN
Chinese Journal of Plastic Surgery 2018;34(7):546-549
Objective:
Explore the method for volumetric measurement of alveolar bone defect.
Methods:
This study applied 2 advanced preoperative volume measurement methods: three-dimensional (3D) printing and computer-aided engineering (CAE). Twenty-six unilateral alveolar cleft patients were enrolled in this study from April 2015 to December 2016. Their computed tomographic data were sent to 3D printing and CAE software. A simulated graft was used on the 3D-printed model, and the graft volume was measured by water displacement. The volume calculated by CAE software used mirror-reverses technique.
Results:
The volume of alveolar bone defect could be detected by both methods. The average volume of the simulated bone grafts by 3D-printed models was 1.61 ml, a little higher than the mean volume of 1.60 ml calculated by CAE software. The difference between the 2 volumes was from -0.34 ml to 0.54 ml. The paired Student