Signal transdcuers and activators of transcription3 (Stat3) is activated by a wide range of cytokines and growth factors following binding to special DNA domain and affects the expression of a variety of genes in modulating the process of cell proliferation, differentiation, and apoptosis. As a point of convergence for many oncogenic tyrosine kinase signaling pathways,it can be activited by sevral accesses. Stat3 is not only in-volved in the function of both apoptosis suppression,proliferation promotion,but also participates the process of angiogenesis,invasion and immune evasion. For repression of this way maybe a new target for break of cancer in-vasion and metastasis,blockers of Stat3 has become the focus of many researches.

OBJECTIVE To probe the risk factors of the secondary lung infection in patients of postoperative(cranio)-(cerebral) trauma as well as the ways to control them.METHODS A prospective and review study was carried out in 718 cases with cranio-cerebral trauma admitted in our hospital for operation from Jan 2002 to Dec 2004.RESULTS Among 718 cases,39(5.43%) developed lung(infection),occupied 65% of the total number of the hospital(infection) in the neurosurgery.After an integrated(total and systemic) treatment,the cure rate was(69.23%(27 cases);) effective: 15.3%(6);improved: 7.7%(3); died: 7.7%(3).CONCLUSIONS Oxygen(inhalation,) nasal(feeding),sputum drawing out,(cannular) tracheotomy,and use of respirator after operation are the causes of the lung infection and it can be controlled effectively if keeping the surroundings clean,keeping strict ness in disinfection-isolation institution,improving the mangement of respiratory tract and the care of oral cavity,proper use of antibiotics,and improving immunity.

Objective To explore the relationship between excessive gestational weight gain and the risk of macrosomia. Methods Three databases were systematically reviewed and reference lists of relevant articles from January 2001 to December 2014 were checked. Meta-analysis of published epidemiological studies (cohort and case-control studies) was done. The odds ratio (OR) was used to evaluate the relationship of excessive gestational weight gain and macrosomia delivery. Results Twelve relevant articles were included to analyze, including 4 cohort studies and 8 case-control ones. The values of OR and 95%CI of macrosomia in pregnant women with excessive and non-excessiveweight gain were 1.66 (1.49, 1.83). Conclusion The risk of macrosomia in pregnant women with excessive gestational weight gain is much higher than those with normal gestational weight gain.

AIM To analyze the effects of glycyrrhetic acid (GA) on c-fos expression and cell proliferation in rat vascular smooth muscle cell (VSMC), and to figure out the mechanisms of binding GA to angiotensin Ⅱ(AⅡ) receptor. METHODS Primary cell culture of rat VSMC, Northern blot, TdR incorporation DNA assay and MTT assay were used in this study. RESULTS ① GA at both low (1?10 -9 mol?L -1) and high concentration (1?10 -5 mol?L -1) quickly induced c-fos expression in VSMC. Sar-AⅡ(1?10 -5 mol?L -1)inhibited both GA induced and AⅡ (1?10 -5 mol?L -1) induced c-fos expression. GA enhanced AⅡ induced c-fos expression at both low and high concentration in VSMC. ② Low level of GA stimulated the proliferation of VSMC. This stimulatory effect decreased with increasing GA concentration, and changed to be inhibitory at high concentration of GA. Not only did Sar-AⅡ eliminate the stimulatory effect of low concentration of GA on cell proliferation, it also eliminated the inhibitory effect of high concentration of GA. Low concentration of GA enhanced the stimulation of AⅡon cell proliferation, while the inhibitory effect of high concentration of GA on cell proliferation was relieved by adding 1?10 -7 mol?L -1 AⅡ. CONCLUSION This study suggests that GA activates transcription factor c-fos and promotes the proliferation of VSMC. GA may exert its effects on cells through AT 1 receptor since it induces similar changes as AⅡ and its effects can be inhibited by AT 1 receptor antagonist.

@@

Objective:To study the effect of injection bovine retinal soluble antigen (S-Ag)into anterior chamber(AC)in inducing immunological tolerance.Methods:S-Ag was extracted from fresh bovine retina and inoculated into the AC of Wistar rats,One week later,the S-Ag mixed with complete fruend's adjuvant(CFA)was injected into footpad intradermaly.The clinical symptoms and the pathological changes were observed.The IL-4 and IFN-? concentrations in the supernatant of splenic cell culture were measured by ELISA.Results:The incidence of EAU in pretreated group(group A) was markedly higher than that of positive controls(gropu B).There were histopathological changes in all rats with clinical symptoms.The IL-4 level of splenic cell culture in group A were significantly higher than those in group B ( P

Objective To investigate the effect of radiation-induced heart injury on cardiac troponin I (cTnI)and endothelin-1(ET-1) and observe the preventive and therapeutic effect of fluvastatin. Methods Healthy female SD rats were randomly divided into three groups: control group(c), irradiation alone group(R) and fluvastatin therapeutic group (F). Rats of F group had been gastrogavaged with fluvastatin at dose of 20mg?kg -1?d -1 from 1week before irradiation to the end of the experiment. In C group and R group, rats were gastrogavaged with the same volume isotonic sodium chloride. The rats of R and F group were irradiated with accelerator linear at a dose of 20Gy thoracically. Rats were executed at 5,15,30d and 60d after irradiation, then cTnI in serum and ET-1 in blood plasma were detected. Results On 5d, the content of cTnI in R group increased significantly than that in C group(P

BACKGROUND:Currently,there are many studies concerning the pathogenesis,process,and damage of glaucoma,however,there is not an ideal glaucoma modelOBJECTIVE:To prepare rabbit intraocular hypertension models using three different material injections,and to verify the practical value of intraocular hypetension modelsMETHODS:Thidy New Zealand rabbits were divided randomly into 3 groups,with 10 animals in each group.One eye of each rabbit was served as the experimental eyes and the other eye as control eyes.Autoblood.methyl cellulose,C3F8 was injected into the anterior chamber of the experimental eyes.and the normal saline was injected into the control eyes.The intraocular pressure(IOP)was monitored prior to injection and at hours 0,24,36,48,72,96.120 and 168 after injection.RESULTS AND CONCLUSION:Intraocular hype Rension models could be induced by injecting 3 kinds of materials,and the IOP was obviously increased after injection(P＜0.05),and the ranges and periods of increasing were varied.The periods of increasing of 3 materials were 1,3 and 7 days,respectively,which could maintain for longer time for a second injection.The IOP ranged 1.86-6.65 kPa,and mild anterior segment inflammation could be found.The experiment demonstrated that intraocular hypeansion models using three different material injections are ideal models,which is characterized by simple,reliable and controllable.The suitable model can be selected for acute or chronic glaucoma research.

Objective The paper focused on teaching status of evening college among different ma-jors, and tried to provide data support and suggestion for teaching reform of evening college in medical university. Method Using methods of questionnaires and interviews, the investigators analyzed five aspects of students in Peking University Health Science Center, which were the influences on study from family and work, and the evaluation on teaching, etc. The investigators entered data with Epidata 3.1 software and de-scribed it with SPSS 20.0 statistical software, with which they also did chi-square and nonparametric rank sum test. Results 38.2% (63/165) of clinical medicine students, 45.8% (27/59) of laboratory medical sci-ence students and 43.0%(71/165) of nursing students regarded hard work and limited energy as their major difficulty from jobs. Limited time for study was treated as the biggest problem for students of clinical medicine (58.2%, 96/165), laboratory medical science (46.5%, 27/58) and nursing (66.5%, 105/158). 41.9%(70/167) of clinical medicine students tended to receive traditional face-to-face teaching; 39.0%(23/59) of laboratory medical science students preferred partial face-to-face teaching with partial network teaching; while 37.2% (68/183) of nursing students tended to partial face-to-face teaching with partial self-study. 58.4%(97/166) of clinical medicine students, 55.9%(33/59) of laboratory medical science students and 64 . 8% ( 118/182 ) of nursing students preferred examination of both memorization and application . Conclusion Results showed that students in different majors presented similar opinions on teaching status, but also with a few dif-ferences. There were the same characteristics between students of clinical medicine and nursing, both with high satisfaction. While students in the laboratory medical science showed the special characteristics, with low satisfaction.

Objective:To study the effects of intrathymical injection allogenic antigen on the establishment of donor specific transplantation immunotolerance.Methods:H 2 antigen extracted from splenic cells of donor C56BL/6 mice was intrathymically injected to recipients adult Balb/c mice, one week later,donor C57BL/6 or C3H skingrafts were transplanted,observing transplanted skin survival times ,meanwhile MLC?CML ?DTH was detected.Results:the median survival time (MST) of transplanted skin allografts was over 70 days in tolerance recipients while the skin MST graft in the control group was 12.6?1.69 days,tolerance recipients normally rejected the donor C3H skin grafts (MST 13.4 ? 1.42 days), MLC detection showed that tolerance recipient splenocyte responded to C57BL/6 stimulator significant reduction ,but in normal reaction to donor C3H stimulator,the CML and DTH of tolerance recipient splenocyte to target cell of donor C57BL/6 was inhabited.The results suggested that slpecific tolerance might be induced by intrathymic injection. Conclusion:The intrathymic injection of donor H 2 antigen extracted from C57BL/6 splenic cells could induce recipients specific tolerance to skin transplantation.