Objective To develop a reversed phase high performance liquid chromatography ( RP￣HPLC) method determining the related substances and levidipine. Methods The Welchrom C18 column (250 mm×4.6 mm,5 μm) was used with Octadecylsilane bonded silica as a filler.The mobile phase consisted of methanol￣ethanol￣water (40:20:40),at the flow rate of 1 mL.min-1 in an isocratic elution,the temperature was at 45 ℃ , and the detective wavelength was 240 nm. Results Levidipine could be separated from all impurities and intermediates within the concentration range from151 to 604 ng.mL-1 , which had a satisfied linear relationship (r= 0.999 9) with a regression equation of Y= 0.049 9X+0.597 9.The LOQ of detection was 31.9 ng.mL-1 . Conclusion The developed method is specific,sensitive,easy and fast to operate,which is suitable for detecting levidipine and its related impurities.

EEG feedback therapy has some value in mental disease treatment. This article designs an EEG feedback system with complete function and good performance based on virtual instruments technology, and brings out project of hardware and software to develop the system. Prototype of the system is made and has some effect of clinic application in mental disease?

Objective This study is to analyze the fundamental frequencies of different speech materials . Methods 60 normal subjects (22 males ,38 females) were studied .When pronouncing /a/,/i/,/u/and the short paragraphs ,the fundamental frequencies were measured using Praat software .Results The analysis of the funda‐mental frequencies for different materials indicates that /i/and /u/were the highest while /a/was the next and the paragraphs were the lowest .For males ,the fundamental frequencies from high to low were:148 .47 Hz ,146 .77 Hz ,136 .33 Hz ,and 132 .85 Hz ;for females ,they were:257 .39 Hz ,250 .52 Hz ,239 .43 Hz and 222 .16 Hz .The experimental results showed that there was no significant difference between the fundamental frequencies of vowels between the two genders but there were significant differences when pronouncing different vowels among the female subjects .Conclusion When measuring fundamental frequencies ,reading short paragraphs can be more accurate and the vowel /a/is more useful in this purpose .

BACKGROUND:Artificial cervical disc prosthesis simulates range of motion and buffer shock function of normal intervertebral discs. Clinical experiments verify that artificial cervical disc prosthesis material has good biocompatibility and mechanical characteristics. OBJECTIVE:To evaluate artificial cervical disc replacement and zero-profile interbody fixation and fusion system for multilevel cervical disease in 2-year folow-up. METHODS:Artificial cervical disc replacement and zero-profile interbody fixation and fusion system were used to treat 42 patients with multilevel cervical disease. The patient presented typical symptoms and signs of spinal cord or nerve root compression. There were 18 cases of cervical myelopathy, 15 cases of nerve root cervical spondylosis and 10 cases of mixed type of cervical spondylosis. After treatment, mean operation time, blood loss and reoperation rate were measured. Postoperative complications, disability index of neck function, visual analog scale, function unit range of corresponding surgery segments of the cervical spine, Cobb angle of C2-C7 vertebral body, range of motion of adjacent segment of proximal and distal vertebral bodies were observed and clinical outcomes were evaluated. RESULTS AND CONCLUSION: Al cases finished the operation and were scored at various time points. After treatment, radiating pain of shoulder and neck and upper extremity were remarkably lessened. Numbness and sensory loss symptoms disappeared obviously. Quality of life elevated noticeably. Visual analog scale and the disability index of neck function score were decreased in final folow-up compared with pre-treatment (P < 0.001). C2-C7 vertebrae Cobb angle, FSU angle, range of motion of proximal surgery adjacent segment and range of motion of the distal surgery adjacent segment were elevated compared with pre-treatment (P < 0.001). These data indicate that cervical spondylosis was improved after treatment. Each index of cervical spondylosis after artificial cervical disc replacement and zero-profile interbody fixation and fusion system was reconstructed to different degrees.

Objective To explore the intervention effect of evidence-based nursing on the health behavior of elderly hypertensive patients in the community.Methods 187 elderly hypertensive patients who were hospitalized in December 2015 and met the inclusive criteria were divided into observation group (n=93) and control group (n=94).The observation group received evidence-based nursing according to the evidence-based medical system evaluation while the control group was given regular nursing both for 6 months.The clinical efficacy of two nursing modes was observed and the differences between pre-intervention and pose-intervention and groups were recorded and analyzed with a self-designed questionnaire, drug compliance questionnaire and health behavior scale.Results In the observation group, the blood pressure was effectively controlled or reduced, treatment compliance was significantly increased and body mass index (BMI) was within the ideal range 6 months later.Scores of unhealthy behaviors such as those in living habits and mental stress were lower than those in the control group, which showed significant difference (P<0.05).There was no significant difference in activity participation between the two groups (P>0.05).Conclusion Evidence-based nursing intervention can effectively control the hypertension, improve the treatment compliance of the community elderly hypertension patients and help lead them to a more positive and healthier life style.

Objective To observe the clinical efficacy of acupuncture in regulating depressed state. Method Sixty patients in depressed state were divided into a treatment group and a control group, 30 cases in each group. The treatment group was given basic intervention plus acupuncture, whereas the control group was treated by basic intervention alone. The two groups were evaluated by using Hamilton Rating Scale for Depression (HAMD) and PSTR prior to treatment, and respectively after 1, 2, and 3-month treatment. The clinical efficacies of the two groups were compared. Result The HAMD and PSTR scores were significantly changed respectively after 1, 2, and 3-month treatment compared to that before treatment in both groups (P<0.05). Respectively after 1, 2, and 3-month treatment, the HAMD and PSTR scores of the treatment group were significantly different from that of the control group (P<0.05). Acupuncture worked rather slowly in the first month of treatment, while in the following 2 months, its efficacy increased significantly. The total effective rate was 93.3% in the treatment group versus 40.0% in the control group, and the difference was statistically significant (P<0.05). There were no adverse reactions during the whole intervention with acupuncture. Conclusion Acupuncture is an effective approach in regulating depressed state.

AIM:To establish HPLC fingerprint for the root and rhizome of Clematis uncinata Champ and to compare the differences of clematis and Clematis uncinata in fingerprint. METHODS:Based on 10 batches of Clematis unciuata Champ,its chromatographic seperation was performed on Diamonsil C18 (250 mm ? 4. 6 mm,5 ?m)with a mobile phase consisting of acetonitrile -0.05% phosphoric acid,gradient eluent,at the flow rate of 0. 8 mL/min. The UV detection was set at 210 nm. RESULTS:The mutual mode to HPLC-UV fingerprints was set up,and the 23 mutual peaks were indicated. The similarities were compared among Cleuatis uncinata Champ and substitutes collected from different sources there were apparent difference in fingerprint. CONCLUSION:The method is stable and reliable with a good reproducibility and provides a reference standard for identifying medicinal clematis.

Objective To investigate the effects of siRNAs targeted protein kinase C ?(PKC?) on the proliferation and apoptosis of A549 cell line.Methods Six PKC? siRNAs were designed and chemical synthesized. Candidate PKC? siRNA was transfected into A549 cells,PKC? mRNA level was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Then,the effects of more effective PKC? siRNAs on A549 were further investigated by using clone formation assay,Hoechst 33258 staining,propidium iodide (PI) staining,Annexin V-FITC and PI dual staining and western blot. Results Six candidate PKC? siRNAs were designed and named as No.1,No.2,No.3,No.4,No.5 and No.6 PKC? siRNA. Compared with controls,PKC? mRNA levels were all significantly downregulated and the cell proliferation was inhibited in A549 cells treated with candidate PKC? siRNAs except No.5 (P

Objective To investigate and analyze the mental health status of the students from poor families and non-poor students by comparative study.Methods Conducting the cluster sampling methods,the author investigated 885 medical students with questionnaires.We input data with Epidata 3.0 software and described it with SPSS 11.0 statistical software,which also did the nonparametric rank sum test.Results 92.7％(667/719) students from poor families considered their economic status among the general level or much lower level.There were 41.9％(294/702) students from poor families who thought families' financial difficulties had positive effects on mental health.92.3％(664/719) students from poor families were never afraid that people around knew they were in work-study program.87.5％(629/719) students from poor families were satisfied with their life.Facing psychological problems,there's no difference with dissatisfaction in daily life and recourses for help between students from poor families and non-poor students except psychological consultation center and lovers P＞0.05).Conclusion Compared with non-poor students,the mental health of students from poor families in medical universities is good.The universities are supposed to pay more attention to the mental health of students who are neither poor nor non-poor and few students from poor families who are negatively impacted by their families' financial difficulties.

Objective To explore the distribution of the liquid food in the stomach of patients with postprandial distress syndrome (PDS)-predominated functional dyspepsia (FD)and the mechanism of its gastric dynamic dysfunction.Methods Fifty-two patients with PDS and 18 healthy volunteers underwent gastric emptying examination with ultrasound scanner provided.The proximal and distal gastric area and volume,area and volume ratio of proximal and distal gastric as well as emptying rate of proximal and distal gastric were calculated at fasting,maximum satiety,30 min,60 min,90 min and 120 min after meal. Student′s t test andχ2 test were performed for statistical analysis.Results Proximal gastric volume of PDS group at the time of fasting and 120 min after meal were (9.06±7.88)and (24.72±24.02)cm3 ,which were larger than those of healthy control group ((5 .96 ± 2.13 )cm3 ,(19.48 ± 12.32 )cm3 ),and the differences were statistically significant (t=-1 .637 and -0.875 ,both P <0.05).Ratio of proximal and distal gastric area at maximum satiety in PDS group was 1 .63±0.42 and that in healthy control group was 2.33±0.63,and the difference was statistically significant (t =5 .044,P <0.05 ).Ratio of proximal and
distal gastric volume in PDS group and healthy group at fasting was 0.60 ± 0.38 and 0.46 ± 0.19, respectively,that at maximum satiety was in PDS group and healthy group 0.91 ±0.27,1 .30 ±0.48, respectively,and the differences were statistically significant (t = - 1 .505 and 3.970,both P <0.05). The emptying rates of proximal stomach of PDS group at 30 min and 90 min postprandial were (28.10 ± 25 .03)% and (68.80±16.14)%,respectively,those of healthy control group were (34.97 ±14.41 )%and (75 .86 ±9.86)%,and the differences were statistically significant (t =1 .087 and 1 .718,both P <0.05).There was no statistically significant difference in emptying rate of distal stomach between two groups (all P > 0.05 ).Conclusions The volume of distal stomach was larger than that of proximal stomach in PDS patients at maximum satiety in liquid food distribution,which may be the cause of decreasing emptying rate of proximal stomach.And proximal stomach dominated gastric motility in PDS patients.