Objective To develop a system achieving automated real-time tracking and localizing of tumor center in radiotherapy. Methods High-accuracy infrared tracking cameras' coordinate system was calibrated with the treatment machine. A kind of passive marker was introduced, which can be imaged by computed tomography and can be localized by the infrared cameras during radiotherapy. Several markers on the surface of the thermal-shaped film were fixed, and then the real-time co-ordinates of the markers was compared with those obtained from the computed tomography. With subsequent space registration, the position biases could be shown finally, which could help achieving the real-time tracking and localizing of the tumor center. Results With infrared tracking cameras and appropriate software, the system can achieve automated and real-time localization in radiotherapy. Conclusion The system is more automated, accurate and speedy than the traditional localization method.

Objective To explore the value of multi-modal MRI in the differential diagnosis of pure fibroadenosis and mixed fibroadenosis in the breast. Methods Forty female patients who underwent 3.0 T MRI within 1 week before sugery and confirmed as breast fibroadenosis by pathology in the General Hospital of the PLA Rocket Force from January 2014 to May 2016 were retrospectively analyzed in this study. There were 20 cases of pure fibroadenosis which including mass type and non-tumor type, 10 cases per type. Twenty cases of mixed fibrous adenosis which including 4 cases of mass type and 16 cases of non-mass type. According to the breast imaging reporting and data system-MRI standard, conventional MRI features, time intensity curve (TIC) types and ADC values of the lesions were observed. MRI features and ages of pure fibroadenosis and mixed fibroadenosis were compared using χ2 test (qualitative data) and independent sample t test (quantitative data), P<0.05 was considered statistically significant. Statistically significant parameters were then used to perform logistic regression analysis to evaluate predictive value . The efficacy of each MRI parameter in the differential diagnosis of pure fibroadenosis and mixed fibroadenosis was analyzed by ROC. Results Statistically significant differencein the size(P<0.05) but no differences in the shape, T2WI manifestation, marginal, internal enhancement, early enhancement curve, and late enhancement (P>0.05) were observed between pure fibroadenosis and mixed fibroadenosis. There was no significant differences in distribution, internal enhancement, early enhancement curve and late enhancement curve between non-tumor type pure fibroadenosis and mixed fibroadenosis (P>0.05). There were significant differences in age, ADC value and peak signal intensity(P<0.05) while no significant differences in early enhancement rate, maximum enhancement rate and peak time (P>0.05) between patients with pure fibroadenosis and mixed fibroadenosis. Logistic regression analysis suggested that the peak signal intensity was closely related to age. It revealed a positive correlation between ADC value, peak signal intensity and the possibility of mixed fibroadenosis. The regression coefficient value, Wals value, and P value of the ADC value were 3.652, 4.363 and 0.034, respectively. The regression coefficient value, Wals value, and P value of the peak signal intensity were 0.005, 5.463 and 0.019, respectively. The area under ROC curve of ADC value, peak signal intensity, ADC value combined with peak signal intensity were 0.697, 0.701 and 0.786, respectively. Conclusions Significantly differences of peak signal intensity and ADC value were observed in mixed fibroadenosis compared with pure fibroadenosis. The combination of ADC value and peak signal intensity had the highest efficacy in predicting pure and mixed fibroadenosis.

OBJECTIVE： To optimize the processing technology of fried Radix Paeoniae， and to provide reference for quality control of the processed products. METHODS： The content of paeoniflorin in fried Radix Paeoniae was determined by HPLC. The determination was performed on Agilent ZORBAX SB-C18 column with mobile phase consisted of acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution （15 ∶ 85， V/V） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃， and detection wavelength was set at 230 nm. The sample size was 10 μL. The appearance character of fried Radix Peaoniae were investigated by appearance color， crosssection color， hardness and smell. Taking the comprehensive score of appearance character and paeoniflorin content as evaluation index， the dosage， stir-frying temperature and stir-frying time were investigated. According to the results of single factor test， Box-Behnken response surface methodology was used to optimize above 3 factors. The optimized processing technology was validated. RESULTS： The linear range of paeoniflorin were 0.02-4.15 mg/mL （r＝0.999 9）； precision， stablity， repeatability and sample recovery rate meet the requirements. The optimal technology of fried Radix Paeoniae included the dosage of 374.60 g， frying temperature of 101.61 ℃， frying time of 20 min. Under optimal technology， comprehensive score of fried Radix Paeoniae ranged 97.39-98.82 in 6 times of parallel verification tests （RSD＝0.54％）， which was close to predicted value 98.18. The color of fried Radix Paeoniae was slightly deeper than Radix Paeoniae， which was crisp and fragrant. CONCLUSIONS： The optimized processing technology of fried Radix Paeoniae is stable and feasible， and is suitable for the preparation of the processed products.