Objective To understand the subjective perception and influential factors of critical care training in rescue nurses. Methods The same self-designed questionnaire with a total of 49 closing entries were used before and after training course to investigate the ability of critical care and prevention ability of infectious diseases in rescue nurses. Results The ability of emergency response nursing improved remarkably after rescue nurse training (t=-7.071--3.693,P<0.01). But there were several weaknesses still in existence including specialty knowledge, first aid techniques, and clinical application of biochemical indicators, psychological trauma assessment, epidemics and ECG recognition, critical
thinking, the differences were statistically significant (t=-6.736--3.286,P<0.01). Conclusions Rescue nurses training can improve the ability to take care of critical illness under emergency situations, which can ease the insufficiency of human resource for short-term.

Objective To measure baseline and influencing factors of core competency of mobile nurses when they join the team and lateral comparison with the domestic nurses,and provide the scientific basis to develop targeted training programs.Methods Using Competency Inventory for Registered Nurse (CIRN) to conducted a questionnaire survey on 56 mobile nurses.Results The core competencies of mobile nurses in our hospital scored (162.82±25.47) points,at a medium level throughout the country.The dimensions of legal and ethical practice were with the highest mean scores,and dimensions of critical thinking and scientific research ability were with the lowest mean scores,professional interests,career development prospects,degree of clinical ability had positive correlation with core competence scores of mobile nurses.Conclusions Core competencies of mobile nurses were at the middle to upper level compared with domestic nurses of the same seniority,but the cultivation for clinical thinking and research ability should be strengthened,and positive encouragement and opportunities for career development of mobile nurses would be necessary.

Objective　This study was to investigate the Chlamydia trachomatis (CT) infection rate in cervical secretion of early pregnant and secondary sterility women. Method　CT-DNA in the cervical secretion of the early pregnant, the secondary sterility, and the control women was detected with polymerase chain reaction (PCR). Results　The infection rates of CT in the early pregnant and secondary sterility women （14.28% and 13.33%）were higher than in the control women (3.33%, P<0.05),and there was no significant difference between the former two groups (P>0.05). Conclusion　There are higher infection rates in the early pregnant and secondary sterility women.Detecting CT and treating in the perinatal period are needed in the early pregnant women who want to be pregnant again.

Objective To study the effects and mechanisms of diet-induced hyperhomocysteinemia on intimal hyperplasia of balloon-injured carotid arteries in rats. Methods Wistar rats were bred with 1 g/(kg?d) L-methionine. After 4 weeks, all animals underwent left common carotid arteries balloon denudation and the left common carotid arteries was extracted at day 14 and 28. The areas of carotid arteries intima and media, the proliferative index of smooth muscle cell and the percentage of reendothelization were measured and calculated. Results Intimal hyperplasia developed in all balloon-injuried arteries. The neointimal hyperplasia in the HHCY group was more obvious than that of the control group. The intimal thickness, neointimal area, Intima/Media area ratio were increased by 36%, 41%, 36% at 14 day and 33%, 30%, 21% at 28 day, respectively. Luminal area was decreased markedly in HHCY group. The percentage of intimal and medial PCNA positive cells increased by 1.7-fold and 2.3-fold at 14day. The percentage of endothelial covering area was decreased by 52% at 14day and 31% at 28day in HHCY group. Conclusion Diet induced hyperhomocysteinemia can stimulate SMC proliferation and inhibit endothelial regeneration, which leads to exacerbated intimal hyperplasia. This study implicates that homocysteine may be a significant risk factor for restenosis following vascular intervention.

Objective To study the expression of IL 1?,TNF ? and nitric oxide (NO) in the cervical intervertebral disc and their roles in the pathogenesis of cervical spondylotic myelopathy (CSM). Methods Fifteen cervical discs were obtained from patients of CSM undergoing anterior disc surgery. Six cervical discs specimens were obtained in the surgical operation with patients with traumatic cervical injury served as the control. The specimens were analyzed for IL 1?、TNF ? by use of ELISA method, NO was measured by optical density assays. Results The contents of IL 1?,TNF ? and NO in disc of CSM patients increased significantly when compared with the control group. Conclusion The results of the present study indicated that IL 1?,TNF ? and NO may be involved in the pathogenesis of CSM.

Objective　To investigate the effect of breast milk and some other kinds of infant foods on the adhesion of EPEC to Hela cells and intestinal mucosa so as to understand the possible protective mechanism of breast-feeding in the intestine of infants. Methods　The adhesion of EPEC to Hela cells and intestinal mucosa were examined by cell culture, bacterial adherence test, light and electron microscopy in the mediums that contain breast milk, cow's milk and formula milk or contain ecal filtrate of the infants fed by breast milk and formula milk respectively. Results　Adhesive index for EPEC to Hela cells was significantly lower in the medium containing breast milk than in control and so did in the fecal medium containing breast fed infants than artificial feeding infants. Conclusion　Breast milk may inhibit the adhesion of EPEC to infant intestine.

Aim To investigate the effect of prenatal alcohol on insulin sensitivity of rat offspring and its underlying mechanism. Methods Female Sprague Dawley rats were given ethanol 4 g?kg-1?d-1 by gavage throughout pregnancy. At 16 wk of age, the rat offsrping underwent intravenous glucose tolerance test. The mRNA level of adipose leptin, resistin and adiponectin were determined by RT-PCR. Serum leptin, resistin and adiponectin were measured with RIA kits. Results Newborn ethanol rats had lower birth weight than control [(5.7?0.1) g vs (6.9?0.1) g,P

Objective To establish a method of primary culture of human preadipocyte in a serum-free medium. Methods Collangenase digestion was used to dispart preadipocyte.The cells were identified by microscopy stained by oil red O and determination of the activity of Glycerol-3-phosphate dehydronase(G-3-PDH). Results The primary cultured human preadipocyte proliferated in the aserum-free medium successfully.In the differential serum-free medium the cells turned to be round on the 4 th day.The adipose drops began to cumulate in the cells,and to the most quantity until the 21 st day.Conclusion The human preadipocyte can be primarily cultured and induced to differentiate in serum-free medium,which is the base for researching the effects of hormones and factors to the proliferation and differentiation of preadipocyte.

Objective To investigate the location and the pathogens of systemic lupus erythematosus (SLE) patients who died from infection.Methods Dead cases of hospitalized SLE patients in Peking Union Medical Hospital from January 1986 to May 2011were retrospectively analyzed.Results Severe infection was an important cause of mortality in patients with SLE.There were 252 dead cases of SLE in total from 1986 to 2011,in which 97 cases died from severe infection.The proportion of patients died from infection was gradually increased during the past 26 years,which was 25.7％,24.1％,31.6％,34.9％ and 60.3％ in 1986-1990,1991-1995,1996-2000,2001-2005 and 2006-2011respectively.Lung was the most common site of infec-t ion,accounting for 65％ of all dead cases.Among the 31cases with identified microorganism,14 patients had single microorganism infection and 17 patients had mixed infections.In the single microorganism infection patients,fungal infection contributed to the cause of death in 9 out of 14 (64％) patients,of which 4,2,1,1,and 1 cases were infected with Pneumocystis carinii, Aspergillus fumigates,Fusarium,Candida tropicalis and Cryptococcus respectively.There were 3 cases of bacteria infection,including 2 cases with Methicillinresistant staphylococcus,aureus and 1case with Klebsiella pneumoniae infection.Two patients died from pulmonary tuberculosis.In 17 patients with mixed infection cases, 14 deaths were caused by bacteria infection,including Acinetobacter baumannii, Eschefichia coli, Enterococcus, Klebsiella pneumoniae,Pseudomonas aeruginosa and Enterobacter cloacae.Eleven patients died from mixed fungus infection,including 6 cases of Aspergillus fumigates,3 cases of Candida tropicalis,3 cases of Pneumocystis carinii,2 cases of Aspergillus flavus,1 case of Soil Aspergillus,2 cases of Candida albicans,1 case of Candida glabrata and Candida Krusei.In addition to bacteria and fungi infection,cytomegalovirus infection occurred frequently in SLE death cases.Conclusions Severe infection has been the most frequent cause of death in SLE patients,in which lung infection is the leading cause.Pneumocystis carinii,Aspergillus fumigates,Acinetobacter baumannii and cytomegalovirus are the major pathogens that cause death in SLE patients.

A porous hydroxylapatite-containing titania film was prepared by an electrochemical oxidation method, i.e. micro-arc oxidation (MAO). During the oxidation treatment, the titanium sample was immersed in electrolytic solution containing calcium acetate monohydrate and sodium biphosphate dihydrate by using a pulse power supply. The thickness, phase, composition and morphology of the oxide coating were monitored with X-ray diffraction (XRD) and scanning electron microscopy (SEM) with energy dispersive X-ray spectrometer (EDS). The thickness of the MAO film is about 20 microm and the coating where each porous size is no more than 5 microm was porous and uneven, without apparent interface to the titanium substrates. The coating formed in the Ca- and P-containing solution with MAO contained Ca and P along with Ti and O. The Ca/P ratio on the surface is 1.63, while that in the interface is 0.51. XRD showed that the porous coating was made up of anatase, rutile and hydroxyapatite. Such MAO films are expected to have significant medical applications as dental implants and artificial bone joints.
Coated Materials, Biocompatible ; chemistry ; Durapatite ; chemistry ; Humans ; Oxidation-Reduction ; Particle Size ; Porosity ; Surface Properties ; Titanium ; chemistry