OBJECTIVE:To discuss the effects of Rujieping capsule on estrogen receptor(ER) and progesteron receptor(PR) of pathological tissues in rats with hyperplasia of mammary glands.METHODS:The rats were injected i.m with Estradiol benzoate injection for 25 days followed by the injection of Progesterone injection i.m for 5 days to establish the model of hyperplasia of mammary glands.Which were treated with high and low doses of Rujieping capsule respectively for 30 days with tamoxifen as positive control.RESULTS:Both tamoxifen and high and low doses of Rujieping capsules could lessen the symptoms of red swelling of nipples or hyperplasia of mammary glands,lower the levels of E2 and PRL,enhance the level of progestone,lower the index of uterus,markedly reduce the number of layers of the ductal epithelial cells,reduce the levels of ER and PR in mammary glands,and suppress mammoplasia.CONCLUSION:Rujieping capsules is effective in the treatment of hyperplasia of mammary glands in rats by regulating the level of estrogen and improve pathomorphologic change of hyperplastic mammary glands.

OBJECTIVE:To study the immunoregularoty effect of Lappaconitine in S180 tumor-bearing mice.METHODS: Each mouse was inoculated hypodermically with S180 tumor cells and normal sodium suspension,24 hours later,all the mice were injected i.m.with Lappaconitine q.d for 10 consecutive days.The immune function of the S180 tumor-bearing mice was detected.RESULTS: In Lappaconitine-treated mice,serum IgG level was heightened significantly;the 2,4-DNFB-induced delayed type hypersensitivity was enhanced;the induction of transformation of lymphocytes into lymphoblast was achieved;the phagocytic function of reticuloendothelial system was enhanced significantly,and the immune function of red cells was promoted.CONCLUSION: Lappaconitine could inhibit the growth of S180 tumor cells and significantly enhance the cellular immune function of S180 tumor-bearing mice.

Objective To improve the diagnostic rate of mammary ductal disease by galactography and mammoscopy.Methods 100 cases of the galactographic and mommoscopy materials were retrospectively analyzed.Results Tumorous diseases including intraductal papilloma,intraductal papillary carcinoma and ductal carcinoma,they accounted for 14%,intraductal papilloma and intraductal papillary carcinoma were the most in this group(12%).Non-tumorous diseases including mammary ductal ectasia with chronicmastitis,plasma cell mastitis,mammary cyst,lobular hyperplasia and cystic hyperplasia,they accounted for 71%,mammary ductal ectasia was the most in this group(42%).Conclusion The galactography and mammoscopy are very valuable in the diagnosis and differentiating diagnosis of mammary ductal diseases.

Methods: Papers on the research of HUA prevention and treatment with medicine-food homology from December 15, 2002 to August 10, 2024 were screened and collected through China National Knowledge Infrastructure (CNKI), PubMed, ScienceDirect, and Google Scholar. Subsequently, the impact of these medications and their extracts, as well as the active compounds on HUA were assessed. Results: A total of 148 relevant papers were collected, including 43 kinds of Chinese medicines and 61 active compounds, all of which have anti-HUA activity. Among them, 41 kinds of Chinese medicines could inhibit the activity of xanthine oxidase, thus leading to the inhibition of uric acid production; and 22 kinds of Chinese medicines could facilitate uric acid excretion, while 15 kinds of Chinese medicines could reduce the inflammation levels in the body and promoting renal protection. Notably, polyphenols and flavonoids are the key active components for the uric acid-lowering effects. Conclusion This study systematically summarized and analyzed the uric acid-lowering effects and mechanisms of Chinese medicines with medicine-food homology, laying a foundation for their development as HUA agents.

BACKGROUND:Bismth-doped iron nanoparticles modified by hyaluronic acid (HA-BiIOPs) not only act as an effective MRI contrast agent, but also as a radiotherapy sensitizer.OBJECTIVE:To fabricate the HA-BiIOPs and to observe its effect to enhance the radiosensitivity of glioblastoma cells U87MG under X-ray radiation.METHODS:HA-BiIOPs were synthesized using hydrothermal polyol method. (1) Cytotoxicity: A cytotoxicity test was carried out on U87MG cells and rat vascular smooth muscle cells (VSMCs). Cell proliferation rate of two kinds of cells cultured with different concentrations of HA-BiIOPs (0, 12.5, 25, 50, 100, 200, 400 mg/L) at 24 hours after culture were determined by cell counting kit-8 assay. (2) Histological analysis: ICR mice were sacrificed after intravenous injection of HA-BiIOPs, and pathological changes of mouse visceral organs were observed under an optical microscope. (3) Cellular uptake: The HA-BiIOPs after entered into the cytoplasm were observed by Prussian blue staining. (4) Radiosensitization test: U87MG cells at Logarithmic growth stage were cultured in culture medium as control group, subjected to X-ray irradiation (0, 3, 6, 9 Gy) as radiotherapy group, cultured in HA-BiIOPs (0, 12.5, 25, 50, 100, 200 and 400 mg/L) as HA-BiIOPs group or subjected to HA-BiIOPs culture plus X-ray irradiation as combined therapy group. Then, the cell proliferation rate and cloning efficiency were measured at 24 hours after treatment.RESULTS AND CONCLUSION:(1) The HA-BiIOPs at different concentrations were non-cytotoxic for VSMC and U87MG cells. (2) After intravenous injection of HA-BiIOPs, there was no obvious toxicity to the mouse susceptible organs. (3) After 6 hours of culture, the HA-BiIOPs could be internalized by U87MG cells. (4) The proliferation rate of U87 cells was negatively correlated with the concentration of HA-BiIOPs (0-200 mg/L) and X-ray dose (0-9 Gy). Especialy, the combination of 6 Gy X-ray irradiation with 200 mg/L HA-BiIOPs dramatically decreased the cell viability that was decreased to (41±7)%. In the combined therapy group with 6 Gy X-ray and 100 mg/L HA-BiIOPs, the cells proliferation rate was significantly lower than that in the control and radiotherapy groups (P < 0.05). These results indicate that HA-BiIOPs have a radiosensitizative effect on glioblastoma cells U87MG.

Objective To explore the skill of mien)surgical removal and preservation and functional recovery of facial nerve for large acoustic neuromas.Methods The clinical data of 82 consecutive patients with large acoustic neuromas operated upon through the suboccipital retrosigmoid approach were retrospectively reviewed.The cases with HB grade Ⅲ - Ⅵ of facial nerve were divided randomly into two groups,the one group was given conventional therapy and the other group was given Kabato rehabilitation training.Results In those cases,acoustic neuromas were totally removed in 77 (93.9%),subtotally removed in 5.Facial nerve was kept anatomically intact in 78 cases of the patients (95.1%).Recovery of facial nerve function was faster and more effective in the training group than that in the un-training group.During the follow-up ranging from 6 months to 3 years,among 5 cases of subtotally resection,2 cases relapsed.Three patients in our series complained of chronic headache postoperatively.No patient long time coma or died.Conclusion The goal of large acoustic neuromas treatment should be total removal in one stage and preservation of facial nerve function,as they determine a patient's quality of life.Kabat rehabilitation training is the effective method of functional recovery of facial nerve after operation.

ObjectiveTo investigate the dietary intake of phytosterols in middle-aged and elderly residents in Guangzhou.Methods The dietary data were collected from 599 middle-aged and elderly residents (222 men and 377 women) recruited by stratified cluster random sampling in Guangzhou.All the subjects were surveyed using Food Frequency Questionnaire (FFQ).The dietary intake of phytosterols was estimated using Chinese data of phytosterol composition of a range of foods.ResultsThe dietary intake of total phytosterols was 336.36 ±142.88 mg/d,which included β-sitosterol 218.53 ± 95.20 mg/d,campesterol 48.33 ± 23.69 mg/d,stigmasterol 36.40 ± 14.38 mg/d,β-sitostanol 30.65 ± 13.62 mg/d,and campestanol 4.67 ±2.77 mg/d.Women had a significantly higher intake of phytosterols than men [ (345.45 ±141.06) mg/d vs.(320.93 ±144.95) mg/d,P=0.0425].Edible oil,vegetable,cereal,and fruit were the four major food sources of phytosterols,representing 37.2％,19.8％,18.5％,and 12.5％ of the total phytosterols intake respectively.Energy-adjusted intake of phytosterols was (42.94 ± 15.66) mg/1000 kJ,and women had a significantly higher intake than men [ (46.04 ±15.90) mg/1000 kJ vs.(37.69 ± 13.76) mg/1000 kJ,P =0.0000 ].ConclusionAmong the middle-aged and elderly residents in Guangzhou,women have higher phytosterols intake than men.

To discuss the relationship between dietary glycemic load (GL) and blood glucose and lipid,201 patients with newly-diagnosed type 2 diabetes and 126 subjects with normal glucose tolerance were enrolled,who were all above 40 years old and permanently lived in communities of Guangzhou.GL,blood glucose and lipid were assessed based on 3-d dietary records.The results showed that the level of dietary glycemic load in diabetic patients was significantly higher than control group (169.61 ± 44.83 vs 157.50 ± 38.47,P＜0.05).Correlation analysis showed that dietary GL was positively correlated with body mass index and HbA1C,and negatively correlated with HDL-C in diabetic patients (all P＜0.05).Adjusted for age and sex by multiple regression,the result was the same.Therefore,dietary with high level of GL may be associated with the occurrence of type 2 diabetes,and is closely related to glucose control and blood lipid metabolism suggesting the necessity to control the level of dietary GL in the nutritional therapy for diabetic patients.

ABSTRACT:Objective To explore the effects of miRNA-1246 (miR-1246)on cell proliferation,invasion and migration in human cervical squamous cell carcinoma (CSCC)cell line SiHa.Methods SiHa cells were assigned into 3 groups:miR-1246 analog group,miR-1246 antagonist group and control group.Transfection efficiency was determined.The MTT assay,transwell assay and wound healing assay were performed respectively to evaluate the proliferation,invasion and migration abilities of SiHa cells.Western blot was carried out to detect the expression of thrombospondin-2 (THBS2)before and after transfection.A THBS2 3’-UTR-containing dual luciferase plasmid was synthesized and co-transfected with miR-1246 into SiHa cells to observe the luciferase enzyme activity.Results MTT assay,transwell assay and wound healing assay revealed that the abilities of proliferation,migration and invasion were significantly enhanced (P<0.01)in SiHa cells transfected with miR-1246 analog,but suppressed in SiHa cells transfected with miR-1246 antagonist.Western blot showed that SiHa cells transfected with miR-1246 analog had significantly decreased THBS2 expression (gray value = 6 .2 8 ± 1 0 .2 2 , P=0 .0 1 3 ) while those transfected with miR-1246 antagonist had significantly increased THBS2 expression (gray value = 12.90±19.81, P=0.037).After co-transfected with miR-1246 and THBS2 3’-UTR-containing plasmid,SiHa cells exhibited a decreased level of luciferase enzyme expression.Conclusion miR-1246 promoted the proliferation,invasion and migration of CSCC SiHa cell, and it might promote CSCC tumorigenesis and progression by suppressing the expression of its target gene THBS2 .

Objective To evaluate the effects of limb ischemic preconditioning (LIP) on the intestinal injury during release of hepatic portal occlusion (RHPO) in rats.Methods Thirty-six male Sprague-Dawley rats,aged 6-7 weeks,weighing 200-250 g,were randomly divided into 3 groups using a random number table:sham operation group (group S,n =12),RHPO group (n =39) and LIP+ RHPO group (n =20).Hepatic ischemia/ reperfusion was produced by occluding portal vein and hepatic artery for 30 min followed by 6 h reperfusion according to Pringle's method.LIP was produced by tourniquet occlusion of the right femoral artery and vein for 10 min followed by 24 h reperfusion before hepatic ischemia.The animals were sacrificed at 6 h after hepatic portal occlusion was released and the mucous membrane of small intestine was obtained for microscopic examination and for determination of malondialdehyde (MDA) content,superoxide dismutase (SOD) activity,myeloperoxidase (MPO)activity,total antioxidant capacity (T-AOC) and intercellular adhesion molecule-1 (ICAM-1) mRNA and protein expression.The degree of damage to intestinal mucous membrane was scored according to Chiu.Results Compared with group S,Chiu's score and levels of MDA and MPO were significantly increased,SOD activity and TAOC were decreased,and ICAM-1 mRNA and protein expression was up-regulated in group RHPO (P ＜ 0.05 or 0.01).Compared with group RHPO,Chiu's score and levels of MDA and MPO were significantly decreased,SOD activity and T-AOC were increased,and ICAM-1 mRNA expression was down-regulated in group LIP + RHPO (P ＜ 0.05).Conclusion LIP can alleviate the intestinal injury during RHPO in rats by enhancing the antioxidant function and down-regulating the expression of ICAM-1.