1.Application of molecular recognition theory in thyrotropin and its receptor system
Fengxian LU ; Chengkai DAI ; Te TANG ; Jing YE ; Wei WANG
Chinese Journal of Pathophysiology 1999;0(09):-
AIM: The selective recognition of the sense peptides which are located in special regions of thyrotropin receptor (TSHR) by their corresponding antisense peptides has been investigated. Three pairs of sense and antisense peptides were named TR1 (aa37-45) and RT1 (aa45-37), TR2 (aa353-366) and RT2 (aa366-353), TR3 (aa648-655) and RT3 (aa655-648). METHODS: To prepare three affinity chromatography columns, antisense peptides were immobilized, called RT1-sepharose 4B, RT2-sepharose 4B and RT3-sepharose 4B, respectively and investigate the retardative behavior for each of native peptide TR1, TR2 or TR3 on above columns with stepwise elution. RESULTS: Each of the three immobilized antisense peptides recognized and retarded its corresponding sense peptide-TR1, TR2 or TR3 instead of those non-complementary peptides. Immobilized RT1 recognized free TSHR protein molecule as well. In additional, bovine thyrotropin was recognized by immobilized TR1. CONCLUSION: The results indicate that molecular recognition theory exsits in thyrotropin receptor system. It may be useful to isolate biological molecules and to locate epitopes of TSH on TSHR molecule. Otherwise, antisense peptide may be used for treatment of experimental autoimmunolized thyroid disease (AITD) in the rat. [
2.Immune regulation of antisense peptides of thyrotropin receptor activity fragments
Fengxian LU ; Chengkai DAI ; Jing YE ; Te TANG ; Wei WANG
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Objective To study the immune regulation of antisense peptide in rats by observing immune function of activity fragments of thyrotropin receptor (TSHR) and their corresponding antisense peptides. Methods TSHR peptides TR1, TR2, TR3 and their antisense peptides RT1, RT2, RT3, and three pairs of complementary peptides were injected into rats of different groups respectively, and the serum levels of TT_3, TT_4, TSHR antibody (TRAb), thyroid stimmulating antibody, thyroid blocking antibody and TSH antibody (TSHAb) and pathological changes in thyroid tissue were investigated. Results Serum TRAb could be induced when each of three fragments of TSHR was injected into rats; TRAb and TSHAb were induced by RT1 or RT2; epithelial hyperplasia and lymphocytic infiltration observed in thyroid tissue of rats injected with TR2 could be abated by injecting RT2 subsequently. Conclusion The results suggest that all 3 TSHR fragments are shown to be immunogenic and are capable to induce TRAb; both RT1 and RT2 show their effect on immune regulation and are idiotypic of TSHR peptides; On the other hand, the humoural and cell immunities are ameliarated by injection of antisense peptides. Therefore, it is possible that antisense peptides may be involved in immune regulation via immune network.
3.Significance of fascin expression and the relationship to estrogen receptor in breast carcinoma
Weiqiang ZHENG ; Jianming ZHENG ; Jian LU ; Fengxian HU ; Chanrong NI ;
Chinese Journal of General Surgery 1994;0(05):-
Objective To study the expression of fascin, an actin bundling protein associated with cell motility, and the relationship with ER negative breast carcinoma.Methods Comparasion of the expression of fascin, estrogen receptor (ER) and proliferative cell nuclear antigen (PCNA) determined by immunohistological method was made on 84 specimens of primary breast carcinomas. Results The diffuse staining of fascin in cancerous cell cytoplasm was noted. Only 3 of 53 breast carcinomas with ER positive showed fascin positive expression (5.67%); in contrast, 21 of 31 carcinomas with ER-negative showed positive expression(67.7 %) (P
4.Nursing about the patch test for 122 patients with chronic eczema
Sulian YANG ; Guoxing ZHU ; Fengxian QU ; Wei LAI ; Chun LU ; Shuxia XIE
Chinese Journal of Practical Nursing 2006;0(05):-
Objective To discuss the key points of nursing the patch test for patients with chronic eczema. Method Analyze the clinical data and the concreted nursing measures which obtained from 122 chronic eczema patients. Result The top 5 chemical allergic resources of these patients were 0.1% thiomersalate, 5% nickel sulfate,7% aromatic compound,1% formaldehyde(water solution),1% styrene. Conclusion\ The key points of getting successful patch test were following the attention issues before,during and after patch test,and to pay attention to the living nursing and explanation of patch test for patients.
5.Nursing about 126 patients with psoriasis vulgaris by methotrexate
Sulian YANG ; Guoxing ZHU ; Fengxian OU ; Wei LAI ; Chun LU ; Peiying FENG
Chinese Journal of Practical Nursing 2006;0(22):-
Objective To study the nursing points of using the methotrexate to cure the psoriasis vulgaris. Methods Analyzed the nursing courses and the clinical datum about 126 patients with psoriasis vulgaris.Results The clinical effects of using the methotrexate to cure the psoriasis vulgaris was satisfactory and safety. The nursing points included psychological nursing for patients, clinical observation during the course of using methotrexate and the self-protection ofnurses.Conclusion Proper nursing and careful using methotrexate can obtain famous curative effect.
6.Expression and significance of ArhGAP29 and E-cadherin in intrauterine adhesions of endometrium
Qian XU ; Hua DUAN ; Sha WANG ; Lu GAN ; Fengxian FU ; Yiqun TANG
Chinese Journal of Clinical and Experimental Pathology 2017;33(1):4-7
Purpose To investigate the expression and significance of ArhGAP29 and E-cadherin in endometrial tissue of intrauterine adhesions (IUAs) and to explore their roles in the pathogenesis of IUAs.Methods The expression of ArhGAP29 and E-cadherin was detected by immunohistochemical PV 9000 two-step method.The correlation between ArhGAP29 and E-cadherin expression and clinical features was analyzed.Results (1) The immunoreactive score (IRS) of ArhGAP29 and E-cadherin in normal endometrial tissue were higher than those in IUAs endometrial tissue (P =0.017,P =0.004).(2) IRS of ArhGAP 29 and E-cadherin in moderate IUAs patients were higher than that in severe IUAs patients (P =0.020,P =0.026).In IUAs patients without amenorrhea,the IRS of ArhGAP29 and Ecadherin were higher (P =0.019,P =0.031) than that in IUAs patients with amenorrhea.(3) The decrease of ArhGAP29 expression had a significantly parallel relationship with the negative expression of E-cadherin (r =0.725,P < 0.001).Conclusion The expression of ArhGAP29 and E-cadherin decreases in endometrial tissue of IUAs patients,which is related with degree of IUAs severity.ArhGAP29 and E-cadherin may participate in the IUAs formation.
7.Curative effect of 308nm excimer laser on child vitiligo and nursing measures
Haiyan CHEN ; Suping GUO ; Fengxian OU ; Sulian YANG ; Chun LU ; Wei LAI
Modern Clinical Nursing 2014;(8):37-39
Objective To explore the curative effect of 308 nm excimer laser on child vitiligo and nursing measures. Method Fourty-eight children with vitiligo were treated with 308 nm excimer laser and nursed with targeted psychological treatment, skin and diet nursing.Result After treatment,6 of them got recovered(12.5%),24 remarkably improved(50%),15 slightly improved(31.2%)and 3 ineffectively treated(6.2%).Conclusions 308 nm excimer laser is effective for the treatment of child vitiligo.High quality nursing can ensure and improve the efficiency of the 308 nm excimer laser.
8.Investigation of leprosy survivors in Fengxian District, Shanghai
Qi SUN ; Qun LU ; Yao-zhong QIAN ; Peng LI ; Wei XU ; Lin ZHANG ; Bai-chen LI ; Xiu LI
Shanghai Journal of Preventive Medicine 2021;33(8):762-766
Objective:To understand the current living and health status of leprosy survivors in Fengxian District, Shanghai, and to provide scientific evidence for improving their quality of life. Methods:In January 2018-June 2020, professionally trained CDC staff performed a household investigation on 41 patients with leprosy (case group) and 82 non-leprosy persons (control group) every year. Living and health status, and routine laboratory examinations such as blood pressure, blood glucose, and blood lipids were monitored. Results:Majority of the patients with leprosy had low educational level (68.29% being primary school) and were mostly farmers (51.22%).The patients had abnormal residual Ⅱ level 5 (12.20%) and mostly were tb-like (TT) (53.66%). Moreover, some patients were not incapacitated (46.34%), had no family financial difficulties (29.27%), did not acquire the national subsistence allowance policy (58.54%), and asked for "regular physical examination" (68.29%).Compared to the 82 control persons, the patients with leprosy had significantly different body mass index (BMI) and blood pressure (
9.Expression of cathepsin G in photoaged fibroblasts
Yue ZHENG ; Wei LAI ; Xiaojian WAN ; Chun LU ; Fengxian OU ; Sulian YANG ; Jinling YI ; Meirong LI ; Chongxiu YE
Chinese Journal of Medical Aesthetics and Cosmetology 2010;16(3):183-186
Objective To investigate the role of cathepsin G in photoaged fibroblasts. Methods Human fibroblasts were cultured and induced to premature senescence using UVA + MOP methods. Senescence-associated-β-galactosidase (SA-β-gal) stain was used to evaluate the positive rate of aged cells. The mRNA and protein expression of cathepsin G in photoaged fibroblasts were detected by real-time RT-PCR and Western blot techniques. Results Over 98 % induced cells presented a positive SA-β-gal straining. The expression of cathepsin G, detected by Western blot, was increased to (1. 70±0. 028) times of the control. And RT-PCR revealed that the synthesis of cathepsin G mRNA was also up-regulated to 1. 42±0. 09. Conclusion The results of our study demonstrates a significant correlation between photoaged fibroblasts and cathepsin G. The up-regulation of cathepsin G may play an important role in the damages of extracellular matrix and activation of MMPS in photoaged human skin.
10.Risk characteristics of hypertension in high-risk population: an analysis based on the surveillance data of chronic diseases in Shanghai
Hai-feng XU ; Hai-ying TANG ; Yuan YUAN ; Fei WU ; Yan LU ; Yu-heng WANG
Shanghai Journal of Preventive Medicine 2021;33(7):553-558
Objective:To understand the epidemiological characteristics of high-risk population of hypertension in Shanghai, and to provide references for health management and targeted lifestyle intervention of the high-risk population in community. Methods:The data from the 2013 Shanghai Non-communicable and Chronic Diseases Surveillance were used. The subjects who met the criteria of high-risk population of hypertension were included, and 11 753 subjects were finally identified, to analyze the distribution and level of risk characteristics. Result:Among six high-risk characteristics of hypertension, the detection rate of high salt in diet was the highest (55.3%), followed by overweight/obesity (44.0%), family history of hypertension (41.6%), and blood pressure level of (130-139)/(85-89) mmHg (31.7%). The detection rate of excessive drinking was the lowest (5.0%). The percentage of population with one or two high-risk characteristics was higher than that with three or more high-risk characteristics (76.9%