Objective To determine the effect of janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway on the expressions of interferon-?(IFN-?) in vital organs of rats with sepsis.Methods A sepsis model was induced by cecal ligation puncture(CLP) in 60 male Wistar rats,and randomly divided into CLP group(n=24),AG490 group in which inhibitor of JAK-AG490 at dose of 8 mg/kg was subcutaneously injected 30 min before CLP(n=18),and rapamycin group in which inhibitor of STAT-rapamycin at dose of 0.4 mg/kg was subcutaneously injected 30 min before CLP(n=18).On 2,6,24 h after CLP,all rats were sacrificed,then hepatic and pulmonary tissue samples were harvested to determine IFN-? mRNA expression levels by reverse transcription polymerase chain reaction(RT-PCR) and its protein expression levels by enzyme-linked immunosorbent assay(ELISA).Results As compared with normal rats without CLP,IFN-? mRNA and protein levels in hepatic and pulmonary tissues at 2,6,24 h after CLP significantly elevated(P

Objective　To evaluate the effect of self-expandable nitinol mesh stent (SENMT) for advanced rectal cancer accompanied with obstruction. Methods　Clinical data were retrospectively analysed on 12 patients with advanced rectal cancer accompanied with acute or chronic obstruction treated by the stent placement. Results　SENMT was placed successfully in 10 patients including replacement in 2 patients because of the stent was migrated. The bowel movement recovered in all of the 10 patients. Ten patients were followed-up, 5 cases died within 56-720 days, and 5 others survived without intestinal obstruction for 6-15 months. The stent failed to be placed in other 2 patients. Conclusions　SENMT may be useful in the management of terminal or high-risk surgical patients for palliative purposes. Palliation management of stent placement combined with chemotherapy and immunotherapy might prolong the survival time of these patients.

Calibration ; Humans ; Poisons ; urine

Objective To evaluate the effect of self expandable nitinol mesh stent (SENMT) for advanced rectal cancer accompanied with obstruction. Methods Clinical data were retrospectively analysed on 12 patients with advanced rectal cancer accompanied with acute or chronic obstruction treated by the stent placement. Results SENMT was placed successfully in 10 patients including replacement in 2 patients because of the stent was migrated. The bowel movement recovered in all of the 10 patients. Ten patients were followed-up, 5 cases died within 56-720 days, and 5 others survived without intestinal obstruction for 6-15 months. The stent failed to be placed in other 2 patients. Conclusions SENMT may be useful in the management of terminal or high risk surgical patients for palliative purposes. Palliation management of stent placement combined with chemotherapy and immunotherapy might prolong the survival time of these patients.

OBJECTIVETo study the influences of carbon disulfide (CS2) exposureon fatty acid metabolism in apolipoprotein E (ApoE) knockout mice and C57BL/6J mice.

METHODSTwenty-four male ApoE knockout mice were randomly and equally divided into four groups: a CS2-exposed normal diet group, a CS2-unexposed normal diet group, a CS2-exposed high-fat diet group, and a CS2-unexposed high-fat diet group. Twenty-four C57BL/6J male mice were divided into four groups in the same way. The CS2-exposed groups were exposed to CS2 (1 g/m³) by static inhalation for 5 hours a day, 5 days a week. After two weeks, the whole blood of mice was collected. Methyl ester derivatization of fatty acids was performed using an acid-catalyzed method. Fatty acid contents before and after exposure were compared by gas chromatography-mass spectroscopy.

RESULTSThere were significant differences in fatty acid contents of mice between the four groups. For the C57BL/6J mice, the arachidic acid contents in the CS2-exposed high-fat diet group were significantly lower than those in the CS2-unexposed high-fat diet group (P = 0.0450). For the ApoE knockout mice, the arachidonic acid contents in the CS2-exposed normal diet group were significantly lower than those in the CS2-unexposed control diet group (P = 0.0452). For the ApoE knockout mice, the γ-linolenic acid contents in the CS2-exposed high-fat diet group were significantly higher than those in the unexposed high-fat diet group (P = 0.0447).

CONCLUSIONExposure to CS2 can induce fatty acid metabolism disorder in mice, indicating that CS2 may increase the risk of atherosclerosis and other cardiovascular diseases.

Animals ; Apolipoproteins E ; drug effects ; Atherosclerosis ; Carbon Disulfide ; toxicity ; Diet, High-Fat ; Gas Chromatography-Mass Spectrometry ; Lipid Metabolism ; drug effects ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Risk Factors