Total 1 500 subjects aged over 60 years were selected from 10 community health service centers with cluster sampling method.A questionnaire survey and physical examination were performed in all subjects.Sixty five patients with chronic heart failure were detected.The questionnaire showed that 77.6％ (38/49)patients were diagnosed in tertiary hospitals,and 12.2％ (6/49) were diagnosed in community hospitals.When heart failure occurred,most patients (37.7％,20/53) chose to take a taxi to the tertiary hospital directly,and less patients (17.0％,9/53)contacted community doctors.When a heart attack happened,68.8％ (33/48)of the patients could reach the hospital within 30 min,and 55.3％ (26/47) within 60 min.45.7％ (21/46) elderly patients were hospitalized for heart failure once a year;31.1％ (14/45) were in hospital for less than 10 d each time.46.0％ (23/50) heart failure patients had received health education and 40.0％ (16/40) had taken drugs under supervision;the majority health educators were community doctors and majority supervisors were family members.The results suggest that the confirmed diagnosis and treatment of heart failure patients still rely on tertiary hospitals,community health institutions play an important role in health education and management of heart failure patients outside hospitals.But the utilization of community health services is still not enough in community health service centers.

Objective To study and prepare the monoclonal antibody library against human FXYD6 functional region,to screen the hybridoma cell lines secreting the monoclonal antibodies against intracellular or extracellular region of human FXYD6,and to identify the biological function of monoclonal antibody against extracellular domain.Methods FXYD6 functional region recombinant protein which did not contain the transmembrane region was prokaryotically expressed,purified,and FXYD6 recombinant protein was used to immunize BALB/c mice.Then splenocytes after immunization were fused with myeloma cells SP2/0.After several rounds of screening and cloning,the hybridomas which secreted the antibodies against the extracellular domain or the intracellular domain of human FXYD6 were established.The antibody specificity and subtype were identified with indirect ELISA,western blot and immunohistochemistry.The monoclonal antibodies against the extracellular domain which recognized the native conformation were screened with flow cytometry.The antibody against extracellular region was prepared with the ascites revulsion method and purified.The affinity constants were measured with indirect ELISA.The function of extracellular monoclonal antibody was detected by HepG2 cell line with high expression of FXYD6.Results The hybridoma cell library which secreted the monoclonal antibody against extracellular domain or the intracellular domain of human FXYD6 was successfully obtained,and extracellular region monoclonal antibodies with the functional blocking were prepared.Conclusion The prepared anti-human FXYD6 extracellular monoclonal antibodies could inhibit HepG2 cell proliferation.