Objective To study the curative effect of transurethral incision for male urethral stricture or atresia. Methods A retrospective) review was made on 55 male patients with urethral stricture or atresia treated by transurethral incision. Results The success rate of the operation on one session was 90.9% (50/55), while the remaining 5 patients were cured by two times of operations.Forty-seven patients were followed for 6~12 months (mean,10 months).Urethral dilatation was performed for once within 1 week after the remorval of catheter in 10 patients,for 3~5 times within 3 months after operation in 21 patients,and after the third postoperative month in 16 patients. Conclusions Transurethral incision for male urethral stricture or atresia is effective.

The current situation of medical information was analyzed by displaying the co-appearance of countries and time, top 5 journals and co-appearance of subject headings in papers on medical information published in 1995-2014 with the papers on medical informatics covered in ISI Web of Science Database as an sample , in order to promote the study and provide reference for the related persons and institutions .

The quality control in novelty assessment of medical literature was analyzed in terms of its strict process, improvement of its staff literacy, and perfection of information resources, with certain suggestions put for-ward its quality control .

BACKGROUND: Group pre-test has confirmed that amnion endothelial cell conditioned medium can induce human umbilical cord blood mesenchymal stem cells into dopaminergic neuron-like cells. In this process, neurotrophic factors and their receptors may play an important role. OBJECTIVE: To study the function of neurotrophic factors secreted by amniotic epithelial cells in the differentiation of human umbilical cord blood mesenchymal stem cells into neurons.METHODS: P1 human umbilical cord blood mesenchymal stem cells at 2×10~8 /L were incubated and assigned to 3 group. Control group was added with HG-DMEM medium. Induction group received human amniotic epithelial cell medium. Blocking agent group underwent blocking agent K252a fluid, and the incubated was conducted at 36 ℃ for 40 minutes, and then amniotic epithelial cell medium was added. Immunofluorescence chemistry was used to determine neuron specific enolase and dopamine transporter expression in human umbilical cord blood mesenchymal stem cells. Real-time quantitative PCR was employed to detect neuron specific enolase, dopamine transporter and tyrosine hydroxylase expression in human umbilical cord blood mesenchymal stem cells. RESULTS AND CONCLUSION: Nerve growth factor and brain-derived neurotrophic factor were observed in human amniotic supernatant. P1 human umbilical cord blood mesenchymal stem cells expressed Trka and Trkb. Forty-eight hours following induction, compared with the control group, positive expression of neuron specific enolase and dopamine transporter was significantly increased in the induction and blocking agent groups (P < 0.05), especially in the induction group (P < 0.05). Neuron specific enolase, dopamine transporter and tyrosine hydroxylase mRNA levels were significantly greater in the induction and blocking agent groups compared with the control group (P < 0.01), and each gene mRNA levels were significantly greater in the induction group than in the blocking agent group (P < 0.01). Results verified that neurotrophic factor in the human amniotic epithelial cells plays important effects on differentiation of human umbilical cord blood mesenchymal stem cells into neurons. The promotion effects are mediated by activating Trk receptor.

Objective To discuss effective nursing measures to reduce incidence rate of anxiety and depression caused by α-intefferen treatment,and to enable the successful completion of interferon treatment for hepatitis B patients. Methods Hepatitis B patients(220 cases) receiving α-interferon treatment were randomly divided into the intervention group(115 cases) and the control group(105 cases).The control group adopted conventional nursing,while in the intervention group,comprehensive intervention measures such as close observation,psychological intervention,symptomatic treatment intervention were used.Zung self-rating depression scale (SDS) was used to compare the incidence rate of anxiety and depression.The completion of treatment was also compared between the two groups. Results After 3,6 and 12 months of treatment incidence rate of anxiety and depression in the intervention group was significantly lower than that of the control group (P＜0.05).There were 112 cases completed treatment in the intervention group,which were higher than those of the control group,72 cases (P＜0.01). Conclusions Nursing intervention measures such as close observation,psychological intervention,symptomatic treatment can reduce incidence rate of anxiety and depression caused by a-interferon treatment.

Objective To explore the effect of quality control circle(QCC) activities for improving the autonomous learning ability of the maxillofacial surgical nurses .Methods Twenty maxillofacial surgical nurses used the steps and methods of QCC activ‐ities .The factors affecting the autonomous learning ability were analyzed and the effective intervention measures were adopted .The activity cycle was 12 months ,including 40 times of theory classes and 40 times of classroom interaction .The scores of nurse autono‐mous learning ability scale ,satisfaction of doctors and patients on surgical nurses were compared between before and after activities . Results The scores of 2 dimensions ,including self motivational belief and self assessment ,after conducting QCC activities were in‐creased compared with before conducting QCC activities ;the doctor′s satisfaction degree and patient′s satisfaction degree were in‐creased ,the differences were statistically significant (P<0 .05) .Conclusion The QCC activities can motivate the working enthusi‐asm and responsibility ,help nurses to increase the autonomous learning ability ,thus enhance the nurses overall quality .

In order to show the development situation and trend in plaque research, Thomson Innovation Platform-covered application of patents associated with plaque researches was quantitatively analyzed using the Thomson Data Analyzer and Thomson Innovation or other tools, which revealed the overall development situation, the main accepted countries, the main application institutions and the technological direction layout of patents associated with plaque researches.

Objective To investigate the cognitive ability and mental health status of the empty-nest elderly people lived in urban area of Beijing.Methods We recruited subjects from 8 communities in Beijing.1067 people were investigated,including 839 empty-nest elderly subjects and 228 not empty-nest elderly subjects.We used demography questionnaire and mental health scale to assess the cognitive ability and mental health of these elders.Results The subjects in the empty-nest elderly having high education,well-condition marriage and being male were more than those in the other group (T or x2=10.769,2.009,159.523,P＜0.05).There was no significant difference in the global cognition between the two groups (F=5.541,P＞0.05).Among language ability,working memory,attention and executive function,the differences between these two groups were significant (F=7.203,4.436,8.806,P＜0.05).Compared with normal elderly subjects,empty-nest elderly subjects had higher scores in face scale and GDS scale (F=5.541,5.578,P＜0.05).Conclusion There are remarkable cognitive ability and mental health differences between empty-nest and not empty-nest elders.

Objective To analyze the institutional cooperation networks of 137 military hospitals in terms of published papers, trends of cooperation and co-authorship in order to guide the cooperation between military hospitals.Methods The institutional cooperation, co-authorship, author-keyword co-occurrence and author-subject co-occurrence in the 137 military hospitals were analyzed with the Thomson data analyzer( TDA) software.Results and Conclusion The institutional coop-eration between military hospitals could be divided into 5 types and were based on the principle of proximity of locations and similiarity of research.The cooperaiton circle with Chinese PLA General Hospital as the center was expanded over time. The institutional cooperation between military and civilian hospitals was also based on the principle of proximity of locations and similiarity of research.Cooperation between productive authors mainly occurred in the same research group but co-au-thorship outside the research group was scarce.The potential competition or cooperation between research fields and authors can be analyzed by comparing co-authorship and author-keyword co-occurrence.

Using cDNA from Rehmannia glutinosa leaf as template, a 972 bp fragment of expansin gene which containing a 762 bp ORF that encoded 253 amino acids, was cloned, named RgEXPA10, which GenBank accession number for this gene is KF011918. A 1 207 bp genomic sequence of RgEXPA10 was amplified by PCR with leaf DNA as template, sequencing analysis revealed that three exons and two introns in RgEXPA10 genomic sequence, and which GenBank accession number is KF011919. Molecular and bioinformatic analyses indicated that RgEXPA10 protein have DPBB_1 and Pollen_allerg_1 domain, also including a 26 aa nuclear localization signal and a 19 aa transmembrane region. Phylogenetic analysis revealed that RgEXPA10 showed the highest homology with AtEXPA8 among the 26 α-expansins in Arabidopsis thaliana. However, the RgEXPA10 indicated the highest homology with the expansin from Solanum lycopersicum among 22 plant species. Expression patterns using qRT-PCR analysis showed that RgEXPA10 mainly expressed in unfolded leaf, followed by the tuberous root at stage of expanding period, and rarely expressed in senescing leaf. And RgEXPA10 showed higher expression level in tuberous root at 60 and 90 days after emergence. The transcription level of RgEXPA10 significantly reduced under all the three stresses including continuous cropping conditions, salinity and waterlogging. This study will lay foundations for molecular function in development and regulation of different stresses for R. glutinosa.