Objective To investigate surgical techniques and curative effects of stereotactic operation in the treatment of cerebellopontine hemorrhage.Methods Ten cases of cerebellopontine hemorrhage were treated by using the model FY-98 Ⅱ stereotactic apparatus.Under the guidance of CT scanning,three-dimension coordinates of the target that was located at the center of the maximum section of the hematoma were calculated.Then a catheter was introduced into the target for aspiration and urokinase irrigation under the guidance of the stereotactic system.Results The operation was successfully completed in all the 10 cases.The operation time was 50~80 min(mean,60 min) and the intraoperative blood loss,25~40 ml(mean,30 ml).Postoperatively,3 fatal cases were encountered because of brainstem function failure or upper digestive tract bleeding.The remaining 7 cases survived after operation and were followed for 3~12 months(mean,8 months).The postoperative hospital stay was 16~30 days(mean,21 days).Assessment with the Activities of Daily Living(ADL) scale showed grade Ⅱ in 3 cases,grade Ⅲ in 2,Ⅳ in 1,and vegetative state in 1.Conclusions Stereotactic surgery in the treatment of cerebellopontine hemorrhage has advantages of accurate location,high reliability,and satisfactory effect.

Objective:To prepare and screen monoclonal antibodies against Herpes simplex virus-1(HSV-1),and develop a double antibody sandwich quantitative enzyme-linked immunosorbent assay( Q-ELISA) for detection of HSV-1 particle. This method was used to control the quality of viral particle in the developing and manufacturing process of HSV-1. Methods: BALB/c mice was immunized with HSV-1 to prepare monoclonal antibodies. A double antibody sandwich Q-ELISA was developed to determine concentration of HSV-1 particle,which was based on the neutralizing monoclonal antibody 1F6 as capture antibody,and 2B1 as HRP-conjugated antibody. The performance of the reagent was evaluated,including specificity,sensitivity,precision,accuracy and linear. And the relation between the amount of virus detected by this method and the virus titer was analyzed by regression analysis method. Results: The Q-ELISA for HSV-1 particle was developed. The quantitation scope was 0. 125-2 μg/ml, the coefficient correlation was 0. 995 5, the limit of detection was 0. 125 μg/ml, the recovery was between 85. 6% and 107. 1%, the variation coefficient was lower than 10%, and the reagent does not react with other samples except HSV-1 antigen. This method has a good correlation with virus titer. Conclusion:The Q-ELISA for HSV-1 particle was successfully developed,which provide a new approach for rapid and quantitative detection of HSV-1 antigen.