Purpose:To study the growth suppressing effect of sodium butyrate(NaB) on human ovarian carcinoma KK cells and endometrial carcinoma HHUA cells and its mechanism, as well as its potential as a new antitumor agent. Methods:Human endometrial carcinoma cell line HHUA and ovarian carcinoma cell line KK were cultured in vitro and exposed to sodium butyrate. The changes of morphology and chromatin induced by NaB were investigated by means of HE staining and DNA fluorescent staining, respectively. Cell cycle distribution and apoptosis were quantified by using flow cytometric analysis. Apoptotic degradation of DNA was analyzed by extracting DNA and separated by electrophoresis through a 2% agarose gel. Western blotting analysis was carried out to determine the expression of PARP, Fas, Bax and Bcl 2 proteins.Results:NaB arrested HHUA and KK cells at G 1 phase at the low concentration (≤2 mol/L), after 24 hours treatment. The percentage of G 1 phase was up to 70%. While at the medium concentration (4 mol/L and 10 mol/L), both HHUA and KK cells manifested typical apoptotic morphological and chromatic features. High concentration (≥10 mol/L) caused cell necrosis. NaB could upregulate Fas protein expression in HHUA, whereas the levels of Bcl 2 and Bax proteins remained unchanged. Conclusions:NaB may suppress the growth of HHUA and KK cells through arrest of cell cycle and induction of apoptosis. The responses of cells to NaB vary at the different concentrations. NaB induces cell cycle arrest at low concentration and induces apoptosis at medium concentration. The inhibition of NaB on cell growth is in a time and dose dependent manner. Upregulation of Fas protein may be the mechanism of apoptosis induction in HHUA cells. The effects of NaB on HHUA and KK cell growth suggest that NaB may be a new therapeutic agent in cancer treatment.

Objective To investigate the prevalence,related factors and individualized health management strategy of female urinary incontinence.Methods Questionnaire survey has been adopted on 4105 female subjects in the east coastal,middle and southwest areas of Shandong Province from Jan.2011 to Dec.2011.Logistic regression model was used to analyze the influencing factors of urinary incontinence.The degree of risk was conducted according to the UI affected to the quality of life.Results The available questionnaires were 4105.Logistic analysis showed that age,delivery frequency,first delivery age,newborn birth weight,perineum injury history,chronic cough and constipation were risk factors of urinary incontinence.However,the protective factors of urinary incontinence included abdominal delivery and whitecollar worker.Mild,moderate and strong impacts of urinary incontinence on the quality of life were found 77.68％ (1079/1389),21.38％ (297/1389) and 0.94％ (13/1389) of the participants,respectively.Unfortunately,doctor's office visiting was as low as 1.08％ (15/1389).Along with the increase of age,parity,age of first time delivery,injury of perineum,chronic cough,constipation,may add the risk of incidence of urinary incontinence while caesarean section and brainwork may decrease the risk of incidence of urinary incontinence.Conclusions The overall of incidence of urinary incontinence in Shandong province is in a high level.Urinary incontinence may significantly reduce the quality of life of the patients.More investigation on individualized health management strategy for female patients with urinary incontinence should be needed in the future.