0.05).Conclusion The group of 13 months age of infants were rarely infected influenza virus in the first time exposure at the age of ≤4 months due to maternal antibodies protection.Whenas,the group of 8 months age who were exposed at the age of ≥5 months,were more easier infected the influenza virus than the group of 13 months age in the first time exposure at the age of ≤4 months.

In this study ,we analyzed the genetic characterizations and propagation paths of measles viruses prevalent in Ningbo ,China from 2004 to 2013 .Measles viruses were isolated from throat swab specimens from 2004 to 2013 ,and 450 bp fragments of C terminus of nucleoprotein (N) gene were amplified by RT-PCR .Sequence analysis was conducted of all 31 virus strains ,and then compared with other measles virus strains published in GenBank .Results showed that all the 31 measles virus isolates belonged to genotype H1 ,6 strains (one strains in 2004 and 5 ones in 2005) belonged to H1b subtype ,and others be-longed to H1a subtype .Compared the 31 strains to the China 93-4 and China 94-7 ,the homology of 450 bp fragments of C ter-minus of nucleoprotein (N) gene were 97 .1%-100% and 96 .7%-100% ,respectively .The homology of 450 bp fragments of nu-cleotide and nucleotide (amino acids) compared with S191 were 81 .9%-92 .4% and 87 .2%-90 .6% ,respectively .It’s suggested that genotype H1 measles virus circulated in Ningbo from 2004 to 2013 ,and H1a was the predominant epidemic strain ,and H1b strain was existed as well .There are five different transmission chains of H1a subtype caused measles co-circulations in Ning-bo .

Objective To investigate the clinical application and manifestation of dynamic contrastenhanced MRI (DCE-MRI) in differentiating true progession from pseudoprogression in patients with gliobastomas.Methods Twenty five glioma patients were treated with postoperative concurrent chemoradiotherapy and enrolled in this study.All patients were underwent DCE-MRI using a 1.5T scanner.Fifteen patients were confimmed by secondary pathology or clinical and imaging follow-up of patients with gliomas true progession (TP),10 patients were pseudoprogress (PP).Nonparametric Mann-Whitney test was used to compare perfusion parameters between two groups (TP and PP),were used for receiver operating characteristic (ROC) curve analysis to clear if these parameters can be the indicators to differentiate true progession from pseudoprogression.Results Ktrans (volume transfer constant),Ve (fractional volume of extravascular extracellular) values between TP and PP glioma groups were statistically significant,K and Ve values were significantly higher in the TP group than in the PP group (P ＜ 0.05).The areas under the ROC curve are 0.990 and 0.847,respectively.Kep (efflux rate constant) value,Vp (fractional volume of plasma) value in the identification of glioma TP group and PP group was not statistically significant (P ＞ 0.05).Conclusions DCE-MRI can be used to identify glioma TP and PP,Ktrans value and Ve value have clinical significance.

Autoimmune pancreatitis (AIP) is an autoimmune-mediated abnormal chronic inflammatory disorder and is often misdiagnosed as pancreatic neoplastic lesions. With in-depth studies of this disease in recent years, it has been taken seriously by hepatobiliary physicians and surgeons. This article summarizes the clinical features, diagnostic criteria, and treatment methods for autoimmune pancreatitis at the present stage, so as to provide clinicians with diagnosis and treatment experience to reduce clinical misdiagnosis.

Objective:To evaluate the effects of transcutaneous electrical acupoint stimulation (TEAS) on sedation in healthy volunteers.Methods:Forty-six healthy volunteers of both sexes, with the Sleep Self-Rating Scale score 10-30, aged 22-28 yr, with the body mass index 18.5-23.0 kg/m 2, were selected and divided into 2 groups ( n=23 each) using a random number table method: TEAS group (E group) and control group (C group). Bilateral Neiguan and Shenmen acupoints were stimulated for 30 min in group E, while the stimulator was only connected, and no current was given in group C. The bispectral index (BIS)value, heart rate, oxygen saturation and mean arterial pressure were recorded at 10 min in the supine position (T 0) during the quiet period, at 10 min intervals during stimulation (T 1, 2) and at 5 min intervals within 15 min after the end of electrical stimulation (T 3-5). Then the condition whether the patient had fallen asleep was observed, and the patients were followed up on the second day for sleep status and related complications. Results:Compared with the baseline at T 0, the BIS value was significantly decreased at T 2-5 in group E ( P<0.05). The BIS value was significantly lower at T 3, 4 in group E than in group C ( P<0.05). There was no significant difference in sleep status during stimulation and at the night of the trial between the two groups ( P>0.05). Heart rate, oxygen saturation and mean arterial pressure were in the normal range at each time point, and no trial-related complications occurred in the two groups ( P>0.05). Conclusion:TEAS can produce certain sedative effect on healthy volunteers.

Objective To analyze the distribution of syphilis infection, serological test results and HIV infection in inpatients of a general hospital in Chengdu, so as to provide effective theoretical basis for the effective prevention and treatment of syphilis. Methods To collect the clinical data of syphilis infected patients in a general hospital in Chengdu from January 2014 to October 2020.The syphilis infection rate of men and women in different age groups, the distribution of syphilis infection departments, the education level, industry and marriage of the infected people were analyzed.The Department Distribution of syphilis infection,and compare the positive rates of tpsab,TPPA and trust in syphilis infected patients,as well as the combined HIV infection rate in syphilis patients of different genders. Results From January 2014 to October 2020,206455 cases of syphilis were screened in our hospital,of which 4760 cases were infected with syphilis,accounting for about 2.31%;there were 2843 males and 1917 females,with a male to female ratio of 1.48:1;the age distribution of 41-50 years old was the most,followed by 51-60 years old,61-70 years old and 31-40 years old,and the age group of<21 years old was the least;and syphilis serological abnormalities were found in 31-40 and above 61 years old in female group The proportion of Syphilis Serological abnormality was lower than that of male(χ²=4.143,P=0.042;χ²=81.395,P<0.001;χ²=63.699,P<0.001;χ²=24.425,P<0.001),while the proportion of Syphilis Serological abnormality in 21-30 age group was higher than that of male(χ²=4.237,P=0.039).Department Distribution: the top five departments were neurology, anorectal hemorrhoids and fistula, gastrointestinal surgery, orthopedics and urology. Among the 4760 patients diagnosed with syphilis infection, the proportion of those with education level of junior high school or below, service industry and married was higher.The positive rates of tpsab and TPPA were significantly higher than those of trust(χ²=6293.760,P<0.001).Among the 4760 syphilis patients,the proportion of HIV infection in male group was higher than that in female group,and the difference was statistically significant(χ²=102.886,P<0.001). Conclusion The syphilis infection of inpatients in our hospital is mainly middle-aged and old men, low education, people engaged in service industry, and the common departments are neurology department. Therefore, the early popularization and screening of this kind of population is of great significance.

OBJECTIVE： To study the long-term toxicity of Liqi sanjie extractum in rats after intragastric administration， and to provide reference for safety evaluation before clinical practice. METHODS： A total of 160 rats were randomly divided into control group （normal saline） and Liqi sanjie extractum low-dose， medium-dose and high-dose groups （7.828 0， 15.656 0， 31.312 0 g/kg， calculated by crude drug）， with 40 rats in each group. They were given relevant medicine intragastrically once a day from Monday to Saturday. The experimental period was 120 days， and the recovery period was 30 days after the end of the experiment. General information of rats was observed， and body weight and feed consumption of rats were measured once a week. At the 61st day of administration， the end of administration and the end of recovery period， 10， 20 and 10 rats were collected from each group to observe their hematology， blood biochemistry， organ coefficient and histopathology changes. RESULTS： From 61st day to 120th day of administration， the rats of Liqi sanjie extractum high-dose group had hair loss and erection， and recovered after withdrawal of medicine. During medication， the body weight of mice in Liqi sanjie extractum low-dose and medium-dose groups increased faster than control group， while the body weight of rats in Liqi sanjie extractum high-dose group increased slower than control group. Compared with control group， the feed consumption of Liqi sanjie extractum low-dose group increased， while those of Liqi sanjie extractum medium-dose and high-dose groups decreased； the rats were recovered after drug withdrawal. On the 61st day of administration and after the end of administration， some hematological indexes， blood biochemical indexes and organ coefficients of rats in administration group were significantly different from those of control group （P＜0.05 or P＜0.01）. The hematology， blood biochemistry and organ coefficients of rats were basically recovered after the end of the recovery period. The number of erythrocyte， hematocrit， standard deviation of erythrocyte width， albumin， globulin ratio and potassium K+ levels in Liqi sanjie extractum low-dose group were significantly lower than those in control group （P＜0.05 or P＜0.01）. The absolute value of intermediate cells in blood of rats in Liqi sanjie extractum medium-dose group was significantly higher than that of control group （P＜0.05）， and the mean hemoglobin concentration， K+ and uterine coefficient in blood were significantly lower than those of control group （P＜0.05）. The number of white blood cells， absolute value of lymphocyte， absolute value of intermediate cells， the percentage of intermediate cells， prothrombin time and spleen coefficient in Liqi sanjie extractum high-dose group were significantly higher than those in the control group （P＜0.05 or P＜0.01）. Mean hemoglobin concentration， granulocyte percentage， albumin， alkaline phosphatase and K+ were significantly lower than those in the control group （P＜0.05 or P＜0.01）. No abnormalities in systemic autopsy and histopathology were noticed in rats. CONCLUSIONS： Long-term intragastric administration of Liqi sanjie extractum can cause certain toxic reactions in rats， and low dose of Liqi sanjie extractum causes less and lighter toxic reactions which can be automatically recovered after drug withdrawal. It can provide reference for the determination of clinical safe dose.

OBJECTIVE： To investigate the improvement effects of Liqi sanjie granule on liver-qi stagnation model rats. METHODS： According to the weight， totally 80 rats were randomly divided into blank control group （normal saline）， model control group （normal saline）， Xiaoyao pill control group （positive control a， 750 mg/kg ，calculated by crude drug）， Xiaojin pill control group （positive control b， 200 mg/kg， calculated by pill weight）， Liqi sanjie pill control group （prototype control， 1 957 mg， calculated by crude drug） and Liqi sanjie granule low-dose， medium-dose and high-dose groups （978.5， 1 957， 3 914 mg/kg， calculated by crude drug）， with 10 rats in each group. Each group was given medicine 20 mL/kg intragastrically once a day， for consecutive 21 d. 1 h after per medication， liver-qi stagnation model was established in those groups by binding method except for blank control group. The syrup preference of rats was determined by designing syrup preference test. Rattail suspension test was adopted to determine the hanging immobility time and struggling times of mice. Open-field behavior test was used to determine total behavior score so as to judge the extent of liver-qi stagnation and effect of the drug in rats. RESULTS： Compared with blank control group， hanging immobility time of model control group was significantly prolonged， the syrup preference and the total behavior score of open field test were decreased significantly， with statistical significance （P＜0.05 or P＜0.01）. Compared with model control group， the struggling times of rats were increased significantly in Xiaojin pill control group， Liqi sanjie pill control group and Liqi sanjie granule medium-dose group （P＜0.05 or P＜0.01）； the hanging immobility time of Xiaoyao pill control group， Xiaojin pill control group， Liqi sanjie pill control group， Liqi sanjie granule low-dose and medium-dose groups were shortened significantly； syrup preference and total behavior score of open-field behavior test were increased significantly （P＜0.05 or P＜0.01）. Compared with Liqi sanjie pill control group， the struggling times of rats were decreased significantly and hanging immobility time were prolonged significantly only in Liqi sanjie granule high-dose group （P＜0.05 or P＜0.01）； there was no statistical significance in above indexes of rats in Liqi sanjie granule low-dose and medium-dose groups （P＞0.05）. CONCLUSIONS： Liqi sanjie granule can significantly improve liver-qi stagnation caused by binding method， and the effects of low-dose and medium-dose Liqi sanjie granule are similar to those of Liqi sanjie pill.