1.Determination of Puerarin in Jiangzhijianfei Granules by HPLC
Zhongkui GUO ; Fenghui HUANG ; Xin LI
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(12):-
Objective To determine the content of Puerarin in Jiangzhijianfei Granules by high performance liquid chromatography.Methods The determination was conducted by HPLC using a Shim-pack-C18 column(5?m,150 mm?4.6 mm)and a mobile phase of methanol-0.1%Phosphoric acid water(25.3∶74.7).The flow rate was 0.9 mL/min and the detection wavelength was 250 nm.Results Good linear relationship between area and amount was noted for 0.114~0.570?g of Puerarin,with a correlation coefficient of 0.999 6.The average recovery of was 98.74%,RSD was 0.67%.Conclusion The established method is simple,accurate,sensitive and can be applied to the quality control of the preparation.
2.A dual-wavelength spectrophotometry of serum theophylline with concomitant use of ofloxacin
Xian HUANG ; Lingping FANG ; Jianhua XU ; Fenghui YOU ; Xiaofang ZENG
Chinese Pharmaceutical Journal 2001;(3):194-196
OBJECTIVE To develop a method for the determination of theophylline in serum with concomitant use of ofloxacin.METHODS Theophylline was serum was extracted with chloroform-hexane(7∶3,v/v) after some ammonium sulfate was added.It was re-extracted with NaOH solution (0.1 mol*L-1)and detected with a UV spectrophotometer at 275 nm and 299.5 nm respeitively.RESULTS The method was linear over the range of 5.0~40.0 mg*L-1(r=0.9997,n=7).The average recovery of methodology was 100.3%(RSD=1.2%),and the extraction recovery from serum was 87.7%(RSD=4.6%).CONCLUSION This practical method for the determination of serum theophylline can eliminate the interference of ofloxacin and other antibiotics.
3.Relationship between college students’ perceived stress and their suicidal ideation: multiple mediating effect of resilience and depression
Caizhi WU ; Na MA ; Tingting HUANG ; Afang LU ; Zhihong REN ; Fenghui YUAN
Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(3):371-375
【Objective】 To explore the relationship between perceived stress and suicidal ideation as well as the mediating effect of resilience and depression. 【Methods】 A sample of 875 college students was recruited for the study to complete a set of self-report measures of Chinese Perceived Stress Scale (CPSS), the Resiliency Scale of University Students, Suicidal Ideation Questionnaire, and Beck Depression Inventory (BDI). 【Results】 ①College students’ perceived stress and depression had significantly positive correlation with their suicidal ideation, while the students’ resilience had a significantly negative correlation with their perceived stress and depression. ②Perceived stress did not affect suicidal ideation of the college students directly, but indirectly affected their suicidal ideation through three significant mediating pathways: the separate mediating effects of (a) resilience and (b) depression, and the serial mediating effect of (c) resilience and depression. 【Conclusion】 Resilience and depression exert a chain mediating effect between perceived stress and suicidal ideation of college students.
4.Association between nocturnal sleep duration combined with snoring exposure and SGA,LGA in the first trimester of pregnancy based on birth cohort
Fenghui Wang ; Kai Ma ; Lianjie Dou ; Dan Huang ; Ying Pan ; Jijun Gu ; Chaohui Huang ; Anhui Zhang ; Hong Tao ; Jiahu Hao
Acta Universitatis Medicinalis Anhui 2022;57(11):1807-1811
Objective :
To investigate the correlation between nocturnal sleep duration combined with snoring in the first trimester of pregnancyand small for gestational age(SGA) ,large for gestational age(LGA) .
Methods :
Multi- variate Logistic regression model was used to analyze the association between nocturnal sleep duration ,snoring, their combined effects and SGA,LGA.
Results :
Compared to nocturnal sleep duration 7 to 9 h in the first trimester of pregnancy,sleep duration<7 h was positively correlated with SGA in male newborn( OR = 4. 22,95% CI : 1. 69 - 10. 52) ; After stratified by snoring,the sleep duration of snoring women<7 h was positively correlated with SGA ( OR = 5. 68,95% CI : 1. 02-31. 51) ,and the sleep duration of non-snoring women<7 h was positively correlated with LGA ( OR = 2. 10,95% CI : 1. 16 -3. 81) .
Conclusion
Sleep duration<7 h in the first trimester of preg- nancy is a risk factor for SGA and LGA,and snoring may enhance the association between sleep duration<7 h in the first trimester of pregnancy and SGA.Pregnant women should keep adequate nocturnal sleep duration to reduce the risk of abnormal neonatal weight.