Objective To investigate the status quo and influencing factors of compassion fatigue among nurses in psychiatric hospitals. Methods The self-designed demographic questionnaire,compassion fatigue scale,occupation-al identity assessment scale and social support rating scale were employed to investigate 480 nurses in five psychi-atric hospitals from two provinces. Results The scores for each dimension in compassion fatigue scale were as fol-lows:compassion satisfaction(32.26±5.34),occupational burnout(27.67±4.34),and secondary traumatic stress(23.72± 4.41).Age,position,individual monthly income,health status,job satisfaction and occupational identity had significant influence on compassion satisfaction(F=19.351,P<0.001). Individual monthly income,health status,job satisfaction and occupational identity had significant influence on occupation burnout (F=35.705,P<0.001). Individual monthly income, personality and social support had significant influence on secondary traumatic stress(F=17.203,P<0.001). Conclusion Nurses in psychiatric hospitals had severe compassion fatigue and this status was influenced by multiple reasons. Nursing managers should pay great attention to nurses with low monthly income,poor health,low job satisfaction, weak social support and low occupational identity,and take effective measures to decrease compassion fatigue of nurses.

Objective To construct five shRNA-expression plasmids and to investigate the expression of Smad2 in TGF-?/ Smads signal transduction treated with shRNA-expression plasmid.Methods Five shRNA-Smad2 DNA sequences from mRNA sequence of mouse Smad2 gene were designed and synthesized.DNA oligonucleotides encoding an appropriate shRNA were inserted to shRNA expression vector respectively.Five shRNA-Smad2 expression plasmids were obtained and then transfected into NIH/3T3 cells.The suppressed expression of Smad2 was assessed by RT-PCR and Western-blotting.Results The shRNA-expression plasmid numbered 2.4 could markedly reduce the expression of Smad2.The suppression effect of the RNAi-pool composed of four different plasmids was more obvious than that of any single.Conclusion The shRNA-expression plasmids were successfully constructed,which could specifically and effectively suppress the expression of Smad2.The method of using a mixture of RNAi plasmids to improve the RNAi efficiency was established.

OBJECTIVE To explore a more effective method of detecting sleep respiratory events in children. METHODS Thirty-eight children were tested with HypnoPTT and 31 with polysomnography (control). The test parameters and operative methods were compared. RESULTS In addition to the parameters comm.on to both HypnoPTT and polysomnography, inspiratory flow limitation and spontaneous microarousal can be measured using HypnoPTT and fewer electrodes were needed. CONCLUSION HypnoPTT is a convenient method characterized by less sleep disturbance and credible results, rendering it is especially suitable for pediatric cases. Pulse transit time is a useful parameter for diagnosing the sleep respiratory disease.